About: An in vivo cell-based assay for investigating the specific interaction between the SARS-CoV N-protein and its viral RNA packaging sequence

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About: An in vivo cell-based assay for investigating the specific interaction between the SARS-CoV N-protein and its viral RNA packaging sequence Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	An in vivo cell-based assay for investigating the specific interaction between the SARS-CoV N-protein and its viral RNA packaging sequence
Creator	Cho, Yong-Eun Kim, Taeyeon Lee, Mirae Woo, Jiwon Lee, Eunice
source	Elsevier; Medline; PMC
abstract	Abstract The SARS-CoV nucleocapsid (N) protein serves multiple functions in viral replication, transcription, and assembly of the viral genome complex. Coronaviruses specifically package genomic RNA into assembled virions, and in SARS-CoV, it is reported that this process is driven by an interaction between the N-protein and a packaging signal encoded within the viral RNA. While recent studies have uncovered the sequence of this packaging signal, little is known about the specific interaction between the N-protein and the packaging signal sequence, and the mechanisms by which this interaction drives viral genome packaging. In this study, we developed a novel in vivo cell-based assay for examining this interaction between the N-protein and packaging signal RNA for SARS-CoV, as well as other viruses within the coronaviridae family. Our results demonstrate that the N-protein specifically recognizes the SARS-CoV packaging signal with greater affinity compared to signals from other coronaviruses or non-coronavirus species. We also use deletion mapping to identify a 151-nt region within the packaging signal sequence that is critical for N-protein-RNA binding, and conversely, we show that both the N-terminal and C-terminal domains of the N protein are necessary for recognizing the packaging RNA. These results describe, for the first time, in vivo evidence for an interaction between the SARS-CoV N-protein and its packaging signal RNA, and demonstrate the feasibility of using this cell-based assay to further probe viral RNA-protein interactions in future studies.
has issue date	2019-12-10(xsd:dateTime)
bibo:doi	10.1016/j.bbrc.2019.09.115
bibo:pmid	31594639
has license	els-covid
sha1sum (hex)	e74c09fac1f951aa3859885662c702089ba95c80
schema:url	https://doi.org/10.1016/j.bbrc.2019.09.115
resource representing a document's title	An in vivo cell-based assay for investigating the specific interaction between the SARS-CoV N-protein and its viral RNA packaging sequence
has PubMed Central identifier	PMC7092827
has PubMed identifier	31594639
schema:publication	Biochemical and Biophysical Research Communications
resource representing a document's body	covid:e74c09fac1f951aa3859885662c702089ba95c80#body_text
is schema:about of	named entity 'RNA PACKAGING' named entity 'interactions' named entity 'signal' named entity 'time' named entity 'complex' named entity 'probe' named entity 'protein' named entity 'domains' named entity 'demonstrate' named entity 'SPECIFIC' named entity 'VIRIONS' named entity 'PACKAGING' named entity 'IN VIVO' named entity 'GREATER' named entity 'PROTEIN-RNA' named entity 'GENOMIC' named entity 'NOVEL' named entity 'OUR' named entity 'HAVE' named entity 'ABOUT' named entity 'DRIVES' named entity 'VIRAL GENOME PACKAGING' named entity 'RNA' named entity 'N-PROTEIN' named entity 'RESULTS' named entity 'VIRAL REPLICATION' named entity 'TO IDENTIFY' named entity 'DELETION' named entity 'C-TERMINAL' named entity 'UNCOVERED' named entity 'PROCESS' named entity 'MECHANISMS' named entity 'FEASIBILITY' named entity 'PACKAGE' named entity 'SIGNAL' named entity 'CORONAVIRUSES' named entity 'EXAMINING' named entity 'REGION' named entity 'FUNCTIONS' named entity 'EVIDENCE FOR' named entity 'TIME' named entity 'CRITICAL' named entity 'SPECIES' named entity 'MULTIPLE' named entity 'VIRAL RNA' named entity 'THESE' named entity 'VIRAL RNA' named entity 'STUDIES' named entity 'PROTEIN ' named entity 'VIRUSES' named entity 'SEQUENCE' named entity 'INVESTIGATING' named entity 'CELL-BASED ASSAY' named entity 'INTERACTION' named entity 'PROTEIN ' named entity 'ITS' named entity 'SARS-COV' named entity 'DESCRIBE' named entity 'STUDY' named entity 'RNA BINDING' named entity 'COMPARED' named entity 'SARS-COV' named entity 'N-PROTEIN' named entity 'CELL-BASED ASSAY' named entity 'CORONAVIRIDAE' named entity 'LITTLE' named entity 'FAMILY' named entity 'KNOWN' named entity 'USE' named entity 'MAPPING' named entity 'ITS' named entity 'VIRAL GENOME' named entity 'ENCODED' named entity 'N PROTEIN' named entity 'AFFINITY' named entity 'USING' named entity 'PROBE'

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