About: Detection of viromes of RNA viruses using the next generation sequencing libraries prepared by three methods

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About: Detection of viromes of RNA viruses using the next generation sequencing libraries prepared by three methods Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection of viromes of RNA viruses using the next generation sequencing libraries prepared by three methods
Creator	Chen, Ji-Ming Hou, Guang-Yu Wang, Kai-Cheng Zhuang, Qing-Ye Qiu, Yuan Wang, Tong Wu, Run
Source	Elsevier; Medline; PMC
abstract	Abstract Virome (viral megagenomics) detection using next generation sequencing has been widely applied in virology, but its methods remain complicated and need optimization. In this study, we detected the viromes of RNA viruses of one mock sample, one pooled duck feces sample and one pooled mink feces sample on the Personal Genome Machine platform using the sequencing libraries prepared by three methods. The sequencing primers were added through random hybridization and ligation to fragmented viral RNA using a RNA-Seq kit in method 1, through random reverse transcription (RT) and polymerase chain reaction (PCR) in method 2 which was developed in our laboratory, and through hybridization and ligation to fragmented amplicons of random RT-PCR using a single primer in method 3. Although the results of these three samples (nine libraries) all showed that more classified viral families and genera were identified using methods 2 and 3 than using method 1, and more classified viral families and genera were identified using method 2 than using method 3, most of the differences were of no statistical significance. Moreover, 11 mammalian viral genera in minks were possibly identified for the first time through this study.
has issue date	2017-06-02(xsd:dateTime)
bibo:doi	10.1016/j.virusres.2017.05.003
bibo:pmid	28501627
has license	els-covid
sha1sum (hex)	e73e4d8b9133c3dad7eee60e7ddeb295b233e4c8
schema:url	https://doi.org/10.1016/j.virusres.2017.05.003
resource representing a document's title	Detection of viromes of RNA viruses using the next generation sequencing libraries prepared by three methods
has PubMed Central identifier	PMC7111271
has PubMed identifier	28501627
schema:publication	Virus Research
resource representing a document's body	covid:e73e4d8b9133c3dad7eee60e7ddeb295b233e4c8#body_text
is schema:about of	named entity 'hybridization' named entity 'feces' named entity 'virology' named entity 'minks' named entity 'viral' named entity 'ligation' named entity 'sample' named entity 'samples' named entity 'generation' named entity 'LIBRARIES' named entity 'RESULTS' named entity 'DETECTED' named entity 'NEED' named entity 'SAMPLE' named entity 'SINGLE' named entity 'USING' named entity 'METHODS' named entity 'GENOME' named entity 'PRIMERS' named entity 'STUDY' named entity 'PRIMER' named entity 'POSSIBLY' named entity 'VIRAL' named entity 'PERSONAL' named entity 'METHOD' named entity 'FECES' named entity 'STATISTICAL SIGNIFICANCE' named entity 'PLATFORM' named entity 'SEQUENCING' named entity 'VIRAL RNA' named entity 'METHODS' named entity 'NEXT GENERATION SEQUENCING' named entity 'ITS' named entity 'COMPLICATED' named entity 'RANDOM' named entity 'LABORATORY' named entity 'MACHINE' named entity 'DUCK' named entity 'NEXT GENERATION SEQUENCING' named entity 'ADDED' named entity 'TIME' named entity 'HYBRIDIZATION' named entity 'APPLIED' named entity 'SAMPLES' named entity 'POOLED' named entity 'MINK' named entity 'DETECTION' named entity 'CLASSIFIED' named entity 'POLYMERASE CHAIN REACTION' named entity 'USING' named entity 'PREPARED' named entity 'RNA VIRUSES' named entity 'THESE' named entity 'RNA VIRUSES' named entity 'PREPARED' named entity 'FAMILIES' named entity 'MAMMALIAN' named entity 'REVERSE TRANSCRIPTION' named entity 'KIT' named entity 'LIGATION' named entity 'BUT' named entity 'DETECTION' named entity 'MOCK' named entity 'IDENTIFIED' named entity 'MOST OF' named entity 'OUR' named entity 'MINKS' named entity 'VIROLOGY' named entity 'OPTIMIZATION' named entity 'RT-PCR' named entity 'RNA-SEQ ' named entity 'FRAGMENTED' named entity 'LIBRARIES' covid:arg/e73e4d8b9133c3dad7eee60e7ddeb295b233e4c8 named entity 'RT-PCR'

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