About: Oral administration of Lactococcus lactis expressing Helicobacter pylori Cag7-ct383 protein induces systemic anti-Cag7 immune response in mice

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About: Oral administration of Lactococcus lactis expressing Helicobacter pylori Cag7-ct383 protein induces systemic anti-Cag7 immune response in mice Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Oral administration of Lactococcus lactis expressing Helicobacter pylori Cag7-ct383 protein induces systemic anti-Cag7 immune response in mice
Creator	Song, Jae-Young Do, Youn Jun, Kim, Su-Jung Kim, Young Lee, Ji Cho, Myung-Je Lee, Woo-Kon
Source	Medline; PMC
abstract	To express the 3′-region (1152 bp) of the cag7 gene of Helicobacter pylori 51 strain, encoding the C-terminal 383 amino acid (ct383 aa) region of Cag7 protein that is known to cover the needle region of T4SS, in a live delivery vehicle Lactococcus lactis, the cag7-ct383 gene was amplified by PCR. DNA sequence analysis revealed that the amino acid sequence of Cag7-ct383 of H. pylori 51 shared 98.4% and 97.4% identity with H. pylori 26695 and J99, respectively. Intramuscular injection of the GST-Cag7-ct383 fusion protein into a rat could raise the anti-Cag7 antibody, indicating the immunogenicity of the Cag7-ct383 protein. When the cag7-ct383 gene was cloned in Escherichia coli–L. lactis shuttle vector (pMG36e) and transformed into L. lactis, the transformant could produce the Cag7-ct383 protein, as evidenced by Western blot analysis. The Cag7-ct383 protein level in the L. lactis transformant reached a maximum at the early stationary phase without extracellular secretion. The oral administration of the L. lactis transformant into mice generated anti-Cag7 antibody in serum in five of five mice. These results suggest that L. lactis transformant expressing Cag7-ct383 protein may be applicable as an oral vaccine to induce mucosal and systemic immunity to H. pylori.
has issue date	2009-12-01(xsd:dateTime)
bibo:doi	10.1111/j.1574-695x.2009.00605.x
bibo:pmid	19807786
has license	no-cc
sha1sum (hex)	e42fdbf77d44f82c37b8eac791ab3810b19d032c
schema:url	https://doi.org/10.1111/j.1574-695x.2009.00605.x
resource representing a document's title	Oral administration of Lactococcus lactis expressing Helicobacter pylori Cag7-ct383 protein induces systemic anti-Cag7 immune response in mice
has PubMed Central identifier	PMC7110310
has PubMed identifier	19807786
schema:publication	FEMS Immunol Med Microbiol
resource representing a document's body	covid:e42fdbf77d44f82c37b8eac791ab3810b19d032c#body_text
is schema:about of	named entity 'SYSTEMIC' named entity 'needle' named entity 'encoding' named entity 'protein' named entity 'Lactococcus lactis' named entity 'Helicobacter pylori' named entity 'PROTEIN' named entity 'REVEALED' named entity 'GENE' named entity 'ENCODING' named entity 'VEHICLE' named entity 'C-TERMINAL' named entity 'RAT' named entity 'TO EXPRESS' named entity 'J99' named entity 'H. PYLORI' named entity 'AMINO ACID' named entity 'PROTEIN ' named entity '26695' named entity 'AMPLIFIED' named entity 'LIVE' named entity 'SHARED' named entity 'PROTEIN ' named entity 'KNOWN' named entity 'REGION' named entity 'INDICATING' named entity 'DELIVERY' named entity 'AMINO ACID SEQUENCE' named entity 'LACTOCOCCUS LACTIS' named entity 'MICE' named entity 'ORAL ADMINISTRATION' named entity 'IMMUNE RESPONSE' named entity 'HELICOBACTER PYLORI' named entity 'STRAIN' named entity 'THE NEEDLE' named entity 'LACTOCOCCUS LACTIS' named entity 'PCR' named entity 'FUSION PROTEIN' named entity 'IDENTITY' named entity 'GENE OF HELICOBACTER PYLORI' named entity 'IMMUNOGENICITY' named entity 'DNA SEQUENCE ANALYSIS' named entity 'INTRAMUSCULAR INJECTION' named entity 'ANTIBODY' covid:arg/e42fdbf77d44f82c37b8eac791ab3810b19d032c named entity 'delivery vehicle' named entity 'gene' named entity 'amino acid' named entity 'protein' named entity 'protein' named entity 'H. pylori' named entity 'Lactococcus lactis' named entity 'antibody' named entity 'Helicobacter pylori' named entity 'sonication' named entity 'Gram-negative bacterium' named entity 'H. pylori' named entity 'PBS' named entity 'H. pylori' named entity 'significant difference' named entity 'PCR' named entity 'L. lactis' named entity 'kDa' named entity 'FCR' named entity 'H. pylori' named entity 'ribosomal binding site' named entity 'kDa' named entity 'extracellular' named entity 'experimental procedure' named entity 'kDa' named entity 'C57BL/6' named entity 'FCR' named entity 'humoral immune response' named entity 'Plasmodium falciparum'

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