About: DNA nanoscaffold-based SARS-CoV-2 detection for COVID-19 diagnosis

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About: DNA nanoscaffold-based SARS-CoV-2 detection for COVID-19 diagnosis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	DNA nanoscaffold-based SARS-CoV-2 detection for COVID-19 diagnosis
Creator	Liu, Y Sun, W Duan, C Xiang, Y Biosensors, Bioelectronics »more»
source	Elsevier; Medline; PMC
abstract	COVID-19 pandemic outbreak is the most astounding scene ever experienced in the 21st century. It has been determined to be caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). With the global pandemic, the lack of efficient rapid and accurate molecular diagnostic testing tools has hindered the public opportunely response to the emerging viral threat. Herein, a DNA nanoscaffold hybrid chain reaction (DNHCR)-based nucleic acid assay strategy is reported for rapid detection of SARS-CoV-2 RNA. In this method, the DNA nanoscaffolds have been first constructed by the self-assembly of long DNA strands and self-quenching probes (H1). Then, the SARS-CoV-2 RNA will initiate the hybridization of H1 and free H2 DNA probes along the nanoscaffold, and an illuminated DNA nanostring is instantly obtained. By taking advantages of the localization design of the H1 probes and the temperature tolerance of the isothermal amplification, the proposed DNHCR method can detect target at short responding time (within 10 min) and mild condition (15°C–35 °C). Moreover, the reliability of DNHCR method in serum and saliva samples have also been validated. Therefore, DNHCR-based method is expected to provide a simple and faster alternative to the traditional SARS-CoV-2 qRT-PCR assay.
has issue date	2020-07-29(xsd:dateTime)
bibo:doi	10.1016/j.bios.2020.112479
bibo:pmid	32763826
has license	no-cc
sha1sum (hex)	e2da2353b95d95071ecea99495c80cf30dd493f2
schema:url	https://doi.org/10.1016/j.bios.2020.112479
resource representing a document's title	DNA nanoscaffold-based SARS-CoV-2 detection for COVID-19 diagnosis
has PubMed Central identifier	PMC7387931
has PubMed identifier	32763826
schema:publication	Biosens Bioelectron
resource representing a document's body	covid:e2da2353b95d95071ecea99495c80cf30dd493f2#body_text
is schema:about of	named entity 'DNA' named entity 'short' named entity 'traditional' named entity 'assay' named entity 'SARS-CoV-2' »more»

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