About: Real-time fluorescence loop-mediated isothermal amplification assay for direct detection of egg drop syndrome virus

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About: Real-time fluorescence loop-mediated isothermal amplification assay for direct detection of egg drop syndrome virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Real-time fluorescence loop-mediated isothermal amplification assay for direct detection of egg drop syndrome virus
Creator	Li, Gang Liu, Wei Liang, Lin Kassenova, Gulmira Kaziyev, Zhambul Zhao, Lingna Zheney, Makay
Source	Medline; PMC
abstract	BACKGROUND: Egg drop syndrome (EDS), caused by the adenovirus “egg drop syndrome virus” (EDSV) causes severe economic losses through reduced egg production in breeder and layer flocks. The diagnosis of EDSV has been done by molecular tools since its complete genome sequence was identified. In order to enhance the capabilities of the real-time fluorescence loop-mediated isothermal amplification (RealAmp) assay, we aimed to apply the method for direct detection of the EDSV without viral DNA extraction. In order to detect the presence of the EDSV DNA, three pairs of primers were designed, from the conserved region of fiber gene of the EDSV. RESULTS: For our assay, test and control samples were directly used in the reaction mixture in 10-fold serial dilution. The target DNA was amplified at 65 °C, which yield positive results in a relatively short period of 40–45 min. The method reported in this study is highly sensitive as compared to polymerase chain reaction (PCR) and showed no sign of cross-reactivity or false positive results. The RealAmp accomplished specific identification of EDSV among a variety of poultry disease viruses. CONCLUSIONS: The direct RealAmp can be used to detect the presence of EDSV. As our result showed, the RealAmp method could be suitable for the direct detection of other DNA viruses.
has issue date	2018-02-13(xsd:dateTime)
bibo:doi	10.1186/s12917-018-1364-9
bibo:pmid	29439721
has license	cc-by
sha1sum (hex)	e27de1e584ddc112681240ed674464f524869e3a
schema:url	https://doi.org/10.1186/s12917-018-1364-9
resource representing a document's title	Real-time fluorescence loop-mediated isothermal amplification assay for direct detection of egg drop syndrome virus
has PubMed Central identifier	PMC5811957
has PubMed identifier	29439721
schema:publication	BMC Vet Res
resource representing a document's body	covid:e27de1e584ddc112681240ed674464f524869e3a#body_text
is schema:about of	named entity 'viral' named entity 'DNA extraction' named entity 'fluorescence' named entity 'severe' named entity 'chain reaction' named entity 'EDS' named entity 'yield' named entity 'reaction' named entity 'serial dilution' named entity 'Real-time' named entity 'detection' named entity 'DIAGNOSIS' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'MIN' named entity 'DNA' named entity 'EGG DROP SYNDROME' named entity 'DNA VIRUSES' named entity 'SUITABLE' named entity 'ADENOVIRUS' named entity 'AMPLIFIED' named entity 'IDENTIFICATION' named entity 'POSITIVE' named entity 'PAIRS' named entity 'COMPARED' named entity 'YIELD' named entity 'FLUORESCENCE' named entity 'REAL-TIME' named entity 'TO DETECT' named entity 'IDENTIFIED' named entity 'CROSS' named entity 'APPLY' named entity 'ENHANCE' named entity 'SAMPLES' named entity 'DIRECT DETECTION' named entity 'SIGN OF' named entity 'MIXTURE' named entity 'SHORT PERIOD' named entity 'VIRAL DNA EXTRACTION' named entity 'TOOLS' named entity 'CONSERVED REGION' named entity 'ISOTHERMAL AMPLIFICATION ASSAY' named entity 'PRIMERS' named entity 'POULTRY DISEASE' named entity 'SEVERE' named entity 'BACKGROUND' named entity 'STUDY' named entity 'VARIETY' named entity 'FLUORESCENCE' named entity 'DIRECT DETECTION' named entity 'EGG DROP SYNDROME VIRUS' named entity 'CONTROL' named entity 'REACTIVITY' named entity 'POLYMERASE CHAIN REACTION' named entity 'EGG PRODUCTION' named entity 'REDUCED' named entity 'DIRECTLY' named entity 'SERIAL' named entity 'USED' named entity 'FIBER' named entity 'REACTION' named entity 'FOLD' named entity 'MOLECULAR' named entity 'VIRUS' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'ORDER' named entity 'ITS' named entity 'DILUTION' named entity 'DIRECT' named entity 'HIGHLY SENSITIVE' named entity 'OUR' named entity 'SPECIFIC' named entity 'ECONOMIC' named entity 'GENOME SEQUENCE' named entity 'SYNDROME' named entity 'REPORTED'

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