About: Strand-Exchange Nucleic Acid Circuitry with Enhanced Thermo-and Structure- Buffering Abilities Turns Gene Diagnostics Ultra-Reliable and Environmental Compatible

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Strand-Exchange Nucleic Acid Circuitry with Enhanced Thermo-and Structure- Buffering Abilities Turns Gene Diagnostics Ultra-Reliable and Environmental Compatible Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Strand-Exchange Nucleic Acid Circuitry with Enhanced Thermo-and Structure- Buffering Abilities Turns Gene Diagnostics Ultra-Reliable and Environmental Compatible
Creator	Jiang, Yu Bhadra, Sanchita Du, Yan Li, Bingling Tang, Yidan Zhu, Zhentong Ellington, Andrew
Source	Medline; PMC
abstract	Catalytic hairpin assembly (CHA) is one of the most promising nucleic acid amplification circuits based on toehold-mediated strand exchange reactions. But its performance is usually ruined by fluctuated environmental temperatures or unexpected self-structures existing in most real-world targets. Here we present an amide-assistant mechanism that successfully reduces the prevalence of these problems for CHA and maximizes its thermo- and structure- buffering abilities. Such an organic amide-promoted CHA (shortened as OHT-CHA) can unprecedentedly amplify through 4 °C to 60 °C without rebuilding sequences or concerning target complexity. We are then for the first time able to employ it as a direct and universal signal booster for loop mediated isothermal reaction (LAMP). LAMP is one of the most promising point-of-care (POC) gene amplifiers, but has been hard to detect precisely due to structured products and haunted off-target amplicons. OHT-CHA guarantees a significant and reliable signal for LAMP reaction amplified from as little as 10(−19) M virus gene. And one single set of OHT-CHA is qualified to any detection requirement, either in real-time at LAMP running temperature (~60 °C), or at end-point on a POC photon counter only holding environmental temperatures fluctuating between 4 °C to 42 °C.
has issue date	2016-11-04(xsd:dateTime)
bibo:doi	10.1038/srep36605
bibo:pmid	27812041
has license	cc-by
sha1sum (hex)	e1cb86642107f15ec6d854bebf9c8341d1416ef4
schema:url	https://doi.org/10.1038/srep36605
resource representing a document's title	Strand-Exchange Nucleic Acid Circuitry with Enhanced Thermo-and Structure- Buffering Abilities Turns Gene Diagnostics Ultra-Reliable and Environmental Compatible
has PubMed Central identifier	PMC5095676
has PubMed identifier	27812041
schema:publication	Sci Rep
resource representing a document's body	covid:e1cb86642107f15ec6d854bebf9c8341d1416ef4#body_text
is schema:about of	named entity 'GENE' named entity 'TO DETECT' named entity 'DETECTION' named entity 'LITTLE' named entity 'DUE TO' named entity 'REAL-TIME' named entity 'EITHER' named entity 'EMPLOY' named entity 'AT END' named entity 'ONE OF' named entity 'TIME' named entity 'THESE' named entity 'TARGET' named entity 'HOLDING' named entity 'ABILITIES' named entity 'HARD' named entity 'COUNTER' named entity 'PRESENT' named entity 'SIGNIFICANT' named entity 'FLUCTUATING' named entity 'BOOSTER' named entity 'VIRUS' named entity 'BUT' named entity 'PROBLEMS' named entity 'REQUIREMENT' named entity 'LOOP' named entity 'ASSISTANT' named entity 'DIRECT' named entity 'MEDIATED' named entity 'NUCLEIC ACID' named entity 'TURNS' named entity 'ENVIRONMENTAL' named entity 'ABILITIES' named entity 'EXCHANGE' named entity 'GENE DIAGNOSTICS' named entity 'ENHANCED' named entity 'ULTRA' named entity 'STRUCTURE' named entity 'OPEN' named entity 'RELIABLE' named entity 'AMPLIFIED' named entity 'LAMP' named entity 'POC' named entity 'MECHANISM' named entity 'RUNNING' named entity 'STRUCTURE' named entity 'SIGNAL' named entity 'ENVIRONMENTAL' named entity 'PHOTON' named entity 'UNIVERSAL' named entity 'AMPLIFY' named entity 'PREVALENCE' named entity 'STRUCTURED' named entity 'REACTION' named entity 'PRODUCTS' named entity 'POINT-OF-CARE' named entity 'STRAND' named entity 'COMPATIBLE' named entity 'HERE' named entity 'END-POINT' named entity 'ITS' named entity 'CHA' named entity 'TEMPERATURE' named entity 'SINGLE' named entity 'RELIABLE' named entity 'COMPLEXITY' named entity 'AMIDE' named entity 'AMPLIFIERS' named entity 'SET' named entity 'SEQUENCES' named entity 'ORGANIC' covid:arg/e1cb86642107f15ec6d854bebf9c8341d1416ef4 named entity 'open-access' named entity 'experimentally determined' named entity 'genetic loci'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software