About: Localization of the Lys, Asp, Glu, Leu tetrapeptide receptor to the Golgi complex and the intermediate compartment in mammalian cells

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About: Localization of the Lys, Asp, Glu, Leu tetrapeptide receptor to the Golgi complex and the intermediate compartment in mammalian cells Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Localization of the Lys, Asp, Glu, Leu tetrapeptide receptor to the Golgi complex and the intermediate compartment in mammalian cells
Creator	Nilsson, Tommy Krijnse-Locker, Jacomine Ericsson, Maria Goud, Bruno Gritiiths, Gareth Hongl, Wanjin Siew, ~ Ssling, Hans-Dieter Tang, Bor Wong, Heng
Source	Medline; PMC
abstract	The carboxyl-terminal Lys-Asp-Glu-Leu (KDEL), or a closely-related sequence, is important for ER localization of both lumenal as well as type II membrane proteins. This sequence functions as a retrieval signal at post-ER compartment(s), but the exact compartment(s) where the retrieval occurs remains unresolved. With an affinity-purified antibody against the carboxyl-terminal sequence of the mammalian KDEL receptor, we have investigated its subcellular localization using immunogold labeling on thawed cryosections of different tissues, such as mouse spermatids and rat pancreas, as well as HeLa, Vero, NRK, and mouse L cells. We show that rab1 is an excellent marker of the intermediate compartment, and we use this marker, as well as budding profiles of the mouse hepatitis virus (MHV) in cells infected with this virus, to identify this compartment. Our results demonstrate that the KDEL receptor is concentrated in the intermediate compartment, as well as in the Golgi stack. Lower but significant labeling was detected in the rough ER. In general, only small amounts of the receptor were detected on the trans side of the Golgi stack, including the trans- Golgi network (TGN) of normal cells and tissues. However, some stress conditions, such as infection with vaccinia virus or vesicular stomatitis virus, as well as 20 degrees C or 43 degrees C treatment, resulted in a significant shift of the distribution towards the trans- TGN side of the Golgi stack. This shift could be quantified in HeLa cells stably expressing a TGN marker. No significant labeling was detected in structures distal to the TGN under all conditions tested. After GTP gamma S treatment of permeabilized cells, the receptor was detected in the beta-COP-containing buds/vesicles that accumulate after this treatment, suggesting that these vesicles may transport the receptor between compartments. We propose that retrieval of KDEL- containing proteins occurs at multiple post-ER compartments up to the TGN along the exocytotic pathway, and that within this pathway, the amounts of the receptor in different compartments varies according to physiological conditions.
has issue date	1994-12-02(xsd:dateTime)
bibo:pmid	7798312
has license	cc-by-nc-sa
sha1sum (hex)	e14c267f2323c8a137e2698dcf29a87713898166
resource representing a document's title	Localization of the Lys, Asp, Glu, Leu tetrapeptide receptor to the Golgi complex and the intermediate compartment in mammalian cells
has PubMed Central identifier	PMC2120279
has PubMed identifier	7798312
schema:publication	J Cell Biol
resource representing a document's body	covid:e14c267f2323c8a137e2698dcf29a87713898166#body_text
is schema:about of	named entity 'DETECTED' named entity 'DIFFERENT' named entity 'INCLUDING' named entity '28S' named entity 'L CELLS' named entity 'USING' named entity 'AMOUNTS' named entity 'UP TO' named entity 'SMALL' named entity 'IMMUNOGOLD LABELING' named entity 'PROTEINS' named entity 'ARP' named entity 'VIRUS' named entity 'SUBCELLULAR LOCALIZATION' named entity 'LOWER' named entity 'VARIOUS' named entity 'GOLGI STACK' named entity 'ROUGH ER' named entity 'COULD BE' named entity 'TETRAPEPTIDE' named entity 'TISSUES' named entity 'RABL' named entity 'WHERE' named entity 'EXACT' named entity 'TARGETING' named entity 'CONTAINING' named entity 'STRUCTURES' named entity 'TGN' named entity 'TESTED' named entity 'GLU' named entity 'RETRIEVAL' named entity 'carboxyl-terminal' named entity 'sorting' named entity 'demonstrate' named entity 'proteins' named entity 'expressing' named entity 'structures' named entity 'signals' named entity 'infected' named entity 'cells' named entity 'targeting' named entity 'This' named entity 'tissues' named entity 'exocytotic' named entity 'Golgi' named entity 'TANG' named entity 'LYS' named entity 'MAMMALIAN' named entity 'ASP' named entity 'LEU' named entity 'RECEPTOR' named entity 'ER LOCALIZATION' named entity 'THESE' named entity 'MEDIATED' named entity 'FUNCTIONS' named entity 'LABELING' named entity 'CELLS' named entity 'INTERMEDIATE' named entity 'GOLGI COMPLEX' named entity '20%' named entity 'VESICLES' named entity 'STRESS' named entity 'NRK' named entity 'VESICULAR STOMATITIS VIRUS' named entity 'THAWED' named entity 'RECEPTOR' named entity 'BUDS' named entity 'NORMAL' named entity 'CELLS' named entity 'GLU' named entity 'OUR' named entity 'HAVE' named entity 'USE' named entity 'CONDITIONS'

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