About: Identification of two liver proteins recognized by autoantibodies elicited in mice infected with mouse hepatitis virus A59

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About: Identification of two liver proteins recognized by autoantibodies elicited in mice infected with mouse hepatitis virus A59 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Identification of two liver proteins recognized by autoantibodies elicited in mice infected with mouse hepatitis virus A59
Creator	Coutelier, Jean-Paul Mathieu, Patricia Retegui, Lilia Gómez, Karina
Source	Medline; PMC
abstract	Western blot experiments showed that sera from mice infected with the mouse hepatitis virus strain A59 (MHV‐A59) contained autoantibodies (autoAb) that bound to a 40‐kDa protein present in liver and kidney extracts. No reaction was observed with extracts of the heart, muscles, spleen, brain and lung. The Ab cross‐reacted with a 40‐kDa protein from human, rat and sheep liver, but not withliver extracts from the silver side fish (Odontesthes bonariensis). No correlation was found between the development of the hypergammaglobulinemia that followed the viral infection and theoccurrence of the autoAb. Reactive immunoglobulins pertained to the IgG1, IgG2a and IgG2b subclasses, recognized cryptic epitopes and were detected from 10 days up to 8 weeks after MHV‐infection. The 40‐kDa protein was purified from mouse liver extracts by ion‐exchange chromatography, gel filtration and SDS‐PAGE. Because the N‐terminal was blocked, we digested the protein in‐gel with trypsin and sequenced various peptides. Results indicated a 100% homology of sequence between the protein recognized by the autoAb and liver fumarylacetoacetate hydrolase (FAH), the enzyme that mediates the last step of tyrosine catabolism. Additionally, a second protein recognized by the autoAb was detected during FAH purification steps and was identified as liver alcohol dehydrogenase.
has issue date	2001-04-23(xsd:dateTime)
bibo:doi	10.1002/1521-4141(200105)31:5<1447::aid-immu1447>3.0.co;2-6
bibo:pmid	11465101
has license	no-cc
sha1sum (hex)	e123f9273566dfee0f399d4b0acfa8bc78dd2a06
schema:url	https://doi.org/10.1002/1521-4141%28200105%2931:5%3C1447::aid-immu1447%3E3.0.co%3B2-6
resource representing a document's title	Identification of two liver proteins recognized by autoantibodies elicited in mice infected with mouse hepatitis virus A59
has PubMed Central identifier	PMC7163598
has PubMed identifier	11465101
schema:publication	Eur J Immunol
resource representing a document's body	covid:e123f9273566dfee0f399d4b0acfa8bc78dd2a06#body_text
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