About: CD81 Receptor Regions outside the Large Extracellular Loop Determine Hepatitis C Virus Entry into Hepatoma Cells

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About: CD81 Receptor Regions outside the Large Extracellular Loop Determine Hepatitis C Virus Entry into Hepatoma Cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	CD81 Receptor Regions outside the Large Extracellular Loop Determine Hepatitis C Virus Entry into Hepatoma Cells
Creator	Pietschmann, Thomas Marcotrigiano, Joseph Gerold, Gisa Bruening, Janina Banse, Pia Kahl, Sina Lasswitz, Lisa Moeller, Rebecca Khan, Abdul
Source	Medline; PMC
abstract	Hepatitis C virus (HCV) enters human hepatocytes using four essential entry factors, one of which is human CD81 (hCD81). The tetraspanin hCD81 contains a large extracellular loop (LEL), which interacts with the E2 glycoprotein of HCV. The role of the non-LEL regions of hCD81 (intracellular tails, four transmembrane domains, small extracellular loop and intracellular loop) is poorly understood. Here, we studied the contribution of these domains to HCV susceptibility of hepatoma cells by generating chimeras of related tetraspanins with the hCD81 LEL. Our results show that non-LEL regions in addition to the LEL determine susceptibility of cells to HCV. While closely related tetraspanins (X. tropicalis CD81 and D. rerio CD81) functionally complement hCD81 non-LEL regions, distantly related tetraspanins (C. elegans TSP9 amd D. melanogaster TSP96F) do not and tetraspanins with intermediate homology (hCD9) show an intermediate phenotype. Tetraspanin homology and susceptibility to HCV correlate positively. For some chimeras, infectivity correlates with surface expression. In contrast, the hCD9 chimera is fully surface expressed, binds HCV E2 glycoprotein but is impaired in HCV receptor function. We demonstrate that a cholesterol-coordinating glutamate residue in CD81, which hCD9 lacks, promotes HCV infection. This work highlights the hCD81 non-LEL regions as additional HCV susceptibility-determining factors.
has issue date	2018-04-20(xsd:dateTime)
bibo:doi	10.3390/v10040207
bibo:pmid	29677132
has license	cc-by
sha1sum (hex)	e10083014ab2d0c09bd8bed432e8ca883d8d3431
schema:url	https://doi.org/10.3390/v10040207
resource representing a document's title	CD81 Receptor Regions outside the Large Extracellular Loop Determine Hepatitis C Virus Entry into Hepatoma Cells
has PubMed Central identifier	PMC5923501
has PubMed identifier	29677132
schema:publication	Viruses
resource representing a document's body	covid:e10083014ab2d0c09bd8bed432e8ca883d8d3431#body_text
is schema:about of	named entity 'susceptibility' named entity 'infectivity' named entity 'For' named entity 'Hepatitis C virus' named entity 'HCV' named entity 'hepatocytes' named entity 'C. elegans' named entity 'HCV' named entity 'tetraspanin' covid:arg/e10083014ab2d0c09bd8bed432e8ca883d8d3431 named entity 'surface' named entity 'extracellular' named entity 'HCV' named entity 'residue' named entity 'CD81' named entity 'human' named entity 'CD81' named entity 'chimera' named entity 'Receptor' named entity 'CD81' named entity 'tetraspanin' named entity 'infectivity' named entity 'extracellular' named entity 'extracellular' named entity 'HCV' named entity 'chimera' named entity 'C. elegans' named entity 'D. rerio' named entity 'HCV' named entity 'CD81' named entity 'D. melanogaster' named entity 'complement' named entity 'chimeras' named entity 'chimeras' named entity 'CD81' named entity 'Receptor' named entity 'infection' named entity 'Renilla luciferase' named entity 'plasma membrane' named entity 'HCV' named entity 'Uniprot' named entity 'antibody staining' named entity 'antibody staining' named entity 'infectivity' named entity 'Infectivity' named entity 'genotype' named entity 'membrane microdomains' named entity 'glycoprotein' named entity 'infectivity' named entity 'NP-40' named entity 'hydrogen bonds' named entity 'complex formation' named entity 'subgenomic RNA' named entity 'CD81' named entity 'transduced' named entity 'sequence conservation' named entity 'Alexa Fluor' named entity 'intracellular' named entity 'lipoprotein' named entity 'TM4' named entity 'Alexa Fluor' named entity 'cholesterol' named entity 'infection' named entity 'virus entry' named entity 'Vector NTI' named entity 'HCV' named entity 'Cdc42' named entity 'cell surface' named entity 'interact' named entity 'cholesterol' named entity 'tetraspanin' named entity 'clone' named entity '0.01'

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