About: A minor groove binder probe real-time PCR assay for discrimination between type 2-based vaccines and field strains of canine parvovirus

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About: A minor groove binder probe real-time PCR assay for discrimination between type 2-based vaccines and field strains of canine parvovirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A minor groove binder probe real-time PCR assay for discrimination between type 2-based vaccines and field strains of canine parvovirus
Creator	Buonavoglia, Canio Campolo, Marco Decaro, Nicola Desario, Costantina Elia, Gabriella Lorusso, Alessio Martella, Vito Cavalli, Alessandra Roperto, Sante
Source	Elsevier; Medline; PMC
abstract	A minor groove binder (MGB) probe assay was developed to discriminate between type 2-based vaccines and field strains of canine parvovirus (CPV). Considering that most of the CPV vaccines contain the old type 2, no longer circulating in canine population, two MGB probes specific for CPV-2 and the antigenic variants (types 2a, 2b and 2c), respectively, were labeled with different fluorophores. The MGB probe assay was able to discriminate correctly between the old type and the variants, with a detection limit of 10(1) DNA copies and a good reproducibility. Quantitation of the viral DNA loads was accurate, as demonstrated by comparing the CPV DNA titres to those calculated by means of the TaqMan assay recognising all CPV types. This assay will ensure resolution of most diagnostic problems in dogs showing CPV disease shortly after CPV vaccination, although it does not discriminate between field strains and type 2b-based vaccines, recently licensed to market in some countries.
has issue date	2006-05-06(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2006.03.030
bibo:pmid	16682086
has license	no-cc
sha1sum (hex)	e0ef85a120c99787e1ec818a0d0251410d3d04c0
schema:url	https://doi.org/10.1016/j.jviromet.2006.03.030
resource representing a document's title	A minor groove binder probe real-time PCR assay for discrimination between type 2-based vaccines and field strains of canine parvovirus
has PubMed Central identifier	PMC7119799
has PubMed identifier	16682086
schema:publication	J Virol Methods
resource representing a document's body	covid:e0ef85a120c99787e1ec818a0d0251410d3d04c0#body_text
is schema:about of	named entity 'strains' named entity 'CPV' named entity 'CPV' named entity 'A minor' named entity 'real-time PCR' named entity 'LOADS' named entity 'STRAINS' named entity 'DIAGNOSTIC' named entity 'MINOR GROOVE' named entity 'MEANS' named entity 'CONTAIN' named entity 'MINOR GROOVE' named entity 'SPECIFIC' named entity 'DOGS' named entity 'ASSAY' named entity 'QUANTITATION' named entity 'BINDER' named entity 'DISCRIMINATION' named entity 'BINDER' named entity 'CANINE PARVOVIRUS' named entity 'REAL-TIME PCR ' named entity 'STRAINS' named entity 'PROBLEMS' named entity 'A MINOR' named entity 'DOES NOT' named entity 'TYPE 2' named entity 'FIELD' named entity 'PROBE' named entity 'VACCINES' named entity 'POPULATION' named entity 'GOOD' named entity 'FIELD' named entity 'REPRODUCIBILITY' named entity 'COUNTRIES' named entity 'MGB' named entity 'CONSIDERING' named entity 'LICENSED' named entity 'PROBE' named entity 'OLD' named entity 'VARIANTS' named entity 'TAQMAN' named entity 'TYPE' named entity 'DISCRIMINATE' named entity 'COPIES' named entity 'DIFFERENT' named entity 'VIRAL DNA' named entity 'LABELED' named entity 'DETECTION LIMIT' named entity 'PCR ASSAY' named entity 'CANINE PARVOVIRUS' named entity 'COMPARING' named entity 'BASED' named entity 'MARKET' named entity 'PROBES' named entity 'RECENTLY' named entity 'MOST OF' named entity 'CALCULATED' named entity 'CIRCULATING' named entity 'DEMONSTRATED' named entity 'DISEASE' named entity 'TYPE 2' named entity 'VACCINES' named entity 'VACCINATION' named entity 'A MINOR' named entity 'CANINE' named entity 'CPV' named entity 'BASED' named entity 'ENSURE' named entity 'DNA' named entity 'TYPES' named entity 'RESOLUTION' named entity 'ACCURATE' covid:arg/e0ef85a120c99787e1ec818a0d0251410d3d04c0

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