About: Comparison of the anti-allergic activity of Syk inhibitors with optimized Syk siRNAs in FcεRI-activated RBL-2H3 basophilic cells

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About: Comparison of the anti-allergic activity of Syk inhibitors with optimized Syk siRNAs in FcεRI-activated RBL-2H3 basophilic cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Comparison of the anti-allergic activity of Syk inhibitors with optimized Syk siRNAs in FcεRI-activated RBL-2H3 basophilic cells
Creator	Schnapp, Andreas Sanderson, Michael Gelling, Stephen Rippmann, Jörg
Source	Elsevier; Medline; PMC
abstract	Abstract Spleen tyrosine kinase (Syk) binds ITAM-bearing receptors in a wide variety of cell types. One such example is the activation of mast cells, basophils and eosinophils via the stimulation of the FcεRI receptor by IgE/allergen complexes. The possible role of Syk in inflammatory signaling cascades has led to the development of pharmacological agents designed to block the Syk catalytic domain as potential novel therapeutics. Whilst the enzymatic activity of Syk lends towards the design of small-molecule inhibitors, other attention has focused on the possibility of targeting Syk expression using anti-sense oligonucleotides as an alternate means of anti-inflammatory therapy. In this study, we compared the ability of multiple optimized Syk siRNA sequences and small-molecule Syk inhibitors to block FcεRI-mediated signal transduction, degranulation and TNFα secretion in the basophilic cell line RBL-2H3. We also characterized the specificity of each siRNA sequence with regards to off-target induction of the interferon-inducible gene IFIT1. We identified a single siRNA sequence, which displayed a favorable profile of efficient Syk knockdown, blockage of FcεRI-mediated signal transduction, degranulation and TNFα secretion and a lack of IFIT1 induction. The effect of this siRNA was comparable to that of the Syk kinase domain inhibitors BAY61-3606 and R406. The identification of an active and specific Syk siRNA could be a basis for the development of therapeutic Syk siRNAs against inflammatory diseases.
has issue date	2010-12-31(xsd:dateTime)
bibo:doi	10.1016/j.cellimm.2009.12.004
bibo:pmid	20053395
has license	els-covid
sha1sum (hex)	e0a778fcfc70c148e8e0a19a174d06b2a7f25958
schema:url	https://doi.org/10.1016/j.cellimm.2009.12.004
resource representing a document's title	Comparison of the anti-allergic activity of Syk inhibitors with optimized Syk siRNAs in FcεRI-activated RBL-2H3 basophilic cells
has PubMed Central identifier	PMC7124319
has PubMed identifier	20053395
schema:publication	Cellular Immunology
resource representing a document's body	covid:e0a778fcfc70c148e8e0a19a174d06b2a7f25958#body_text
is schema:about of	named entity 'BASOPHILIC' named entity 'ANTI-ALLERGIC ACTIVITY' named entity 'Syk' named entity 'COMPARISON' named entity 'SYK INHIBITORS' named entity 'IGE' named entity 'RBL-2H3' named entity 'SPLEEN TYROSINE KINASE' named entity 'PICEATANNOL' named entity 'FCERI' named entity 'RBL-2H3' named entity 'SIRNAS' named entity 'SYK' named entity 'CELLS' named entity 'ACTIVATED' named entity 'SIRNA' named entity 'FCERI' covid:arg/e0a778fcfc70c148e8e0a19a174d06b2a7f25958 named entity 'tyrosine kinase' named entity 'Syk' named entity 'siRNA' named entity 'Piceatannol' named entity 'siRNA' named entity 'Syk' named entity 'Syk' named entity 'siRNAs' named entity 'basophilic' named entity 'Syk' named entity 'anti-inflammatory' named entity 'TaqMan' named entity 'protein' named entity 'allergic' named entity 'inducible gene' named entity 'LAT' named entity 'siRNA' named entity 'BC4' named entity 'IgE' named entity 'enzyme immunoassay' named entity 'siRNAs' named entity 'siRNAs' named entity 'Billerica' named entity 'Syk' named entity 'primers' named entity 'siRNA' named entity 'Darmstadt' named entity 'Syk' named entity 'Syk' named entity 'Erk' named entity 'RNA Polymerase II' named entity 'mRNA' named entity 'Western blotting' named entity 'kinase inhibitors' named entity 'interferon' named entity 'Syk' named entity 'primer' named entity 'Syk' named entity 'SYK' named entity 'siRNAs' named entity 'double stranded RNA' named entity 'Syk' named entity 'signal transduction' named entity 'siRNAs' named entity 'PCR amplification' named entity 'lupus' named entity 'Syk' named entity 'receptor' named entity 'toll-like receptor 3' named entity 'Syk' named entity 'siRNA' named entity 'Syk' named entity 'R406' named entity 'lysed' named entity 'protein' named entity 'siRNAs' named entity 'inhalation' named entity 'protein' named entity 'TNFa' named entity 'siRNAs'

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