About: Improved detection of human influenza A and B viruses in respiratory tract specimens by hemi-nested PCR

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About: Improved detection of human influenza A and B viruses in respiratory tract specimens by hemi-nested PCR Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Improved detection of human influenza A and B viruses in respiratory tract specimens by hemi-nested PCR
Creator	Puro, Vincenzo Carletti, Fabrizio Selleri, Marina Bevilacqua, Nazario Cappiello, Giuseppina Gualano, Gina Minosse, Claudia Capobianchi, Maria Lauria, Francesco Zaniratti, Maria
Source	Elsevier; Medline; PMC
abstract	RT-PCR is the most sensitive assay for diagnosis of influenza, due to enhanced rapidity and sensitivity as compared to classical methods. Hemi-nested RT-PCR was developed, targeting NP gene for influenza A and NS gene for influenza B, based on a previous single round RT-PCR method. The new method was compared with the previous technique for analytical sensitivity and specificity, and was applied to clinical samples from the lower and upper respiratory tract. The analytical sensitivity of hemi-nested RT-PCR was 10 (influenza A) and 4 times (influenza B) higher than the previous method. A high specificity of the new hemi-nested RT-PCR assay was observed by using whole respiratory viruses. When applied to lower respiratory tract specimens, the new method showed an increased rate of positivity as compared to the previous technique (9.3% versus 0.7% for influenza A, and 0.9% versus 0.2% for influenza B). Screening of upper respiratory tract samples collected during the seasonal 2005–2006 outbreak indicated 26.4% and 5.8% positivity for influenza A and B, respectively. The results were confirmed by sequence analysis: apart from influenza B, both influenza A subtypes H3N2 and H1N1, associated with the seasonal outbreak, were detected.
has issue date	2007-01-25(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2006.12.010
bibo:pmid	17257688
has license	no-cc
sha1sum (hex)	e090b81b322d7a6b08f1e1ea723c60940e23b65b
schema:url	https://doi.org/10.1016/j.jviromet.2006.12.010
resource representing a document's title	Improved detection of human influenza A and B viruses in respiratory tract specimens by hemi-nested PCR
has PubMed Central identifier	PMC7119638
has PubMed identifier	17257688
schema:publication	J Virol Methods
resource representing a document's body	covid:e090b81b322d7a6b08f1e1ea723c60940e23b65b#body_text
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