About: Enhancement of the vaccinia virus/phage T7 RNA polymerase expression system using encephalomyocarditis virus 5'-untranslated region sequences

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About: Enhancement of the vaccinia virus/phage T7 RNA polymerase expression system using encephalomyocarditis virus 5'-untranslated region sequences Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Enhancement of the vaccinia virus/phage T7 RNA polymerase expression system using encephalomyocarditis virus 5'-untranslated region sequences
Creator	Heijnen, Leo Rijnbrand, Rene Vennema, Harry Spaan, Willy Horzinek, Marian
source	Elsevier; Medline; PMC
abstract	Abstract A recombinant vaccinia virus producing the bacteriophage T7 RNA polymerase was used to express foreign genes in eukaryotic cells. Translation efficiency in this expression system was enhanced significantly by employing the encephalomyocarditis virus (EMCV) 5'-untranslated region (UTR) which confers cap-independent translation by directing internal initiation of translation. The enhancement was accomplished by fusing open reading frames (ORFs) to the N terminus of the EMCV polyprotein coding region, thus utilizing its highly efficient translation initiation site. Expression vectors were constructed to allow cloning in all three reading frames. As reporter genes, we used the lacZ gene and a number of genes encoding coronavirus structural proteins: among others the genes encoding glycoproteins with N-terminal signal sequences. The signal sequences of these glycoproteins are located internally in the primary translation product. We demonstrated that this did not interfere with translocation and glycosylation and yields biologically active proteins. The usefulness of sequences that direct internal initiation was extended by using EMCV UTR s to express two and three ORFs from polycistronic mRNAs.
has issue date	1991-12-15(xsd:dateTime)
bibo:doi	10.1016/0378-1119(91)90435-e
bibo:pmid	1660838
has license	els-covid
sha1sum (hex)	df238f71b181f3818d71c00f1c1754c7551e734c
schema:url	https://doi.org/10.1016/0378-1119%2891%2990435-e
resource representing a document's title	Enhancement of the vaccinia virus/phage T7 RNA polymerase expression system using encephalomyocarditis virus 5'-untranslated region sequences
has PubMed Central identifier	PMC7131192
has PubMed identifier	1660838
schema:publication	Gene
resource representing a document's body	covid:df238f71b181f3818d71c00f1c1754c7551e734c#body_text
is schema:about of	named entity 'SEQUENCES' named entity 'UNTRANSLATED REGION' named entity 'vaccinia virus' named entity 'producing' named entity 'T7 RNA polymerase' named entity 'EXPRESSION SYSTEM' named entity 'ENHANCEMENT' named entity 'INDEPENDENT' named entity 'USING' named entity 'UNTRANSLATED REGION' named entity 'TO EXPRESS' named entity 'RECOMBINANT VACCINIA VIRUS' named entity 'ENCEPHALOMYOCARDITIS VIRUS' named entity 'EXPRESSION SYSTEM' named entity 'BACTERIOPHAGE T7 RNA POLYMERASE' named entity 'CAP' named entity 'EUKARYOTIC CELLS' named entity 'VACCINIA VIRUS' named entity 'TRANSLATION' named entity 'USED' named entity 'ENCEPHALOMYOCARDITIS VIRUS' named entity 'EFFICIENCY' named entity 'ENHANCED' named entity 'T7 RNA POLYMERASE' named entity 'PRODUCING' named entity 'INITIATION' named entity 'INTERNAL' named entity 'EMPLOYING' named entity 'FOREIGN' named entity 'GENES' covid:arg/df238f71b181f3818d71c00f1c1754c7551e734c named entity 'internal' named entity 'recombinant' named entity 'Translation' named entity 'T7 RNA polymerase' named entity '5'-untranslated region' named entity 'UTR' named entity 'vaccinia virus' named entity 'encephalomyocarditis virus' named entity 'T7 RNA polymerase' named entity 'encephalomyocarditis virus' named entity 'vaccinia virus' named entity 'FIPV' named entity 'methionine' named entity '5' end' named entity 'vTNZ' named entity 'FIPV' named entity 'mRNA' named entity 'N-terminal' named entity 'translocation' named entity 'reading frames' named entity 'UTR' named entity 'infection' named entity 'vTNZ' named entity 'start codon' named entity 'coinfection' named entity 'vaccinia virus' named entity 'mRNAs' named entity 'Recombinant' named entity 'Metabolic' named entity 'scintillation counting' named entity 'MCS' named entity 'polyprotein' named entity 'glycosylated' named entity 'RIPA' named entity 'cDNA' named entity 'MCS' named entity 'transmembrane segment' named entity 'gene' named entity 'signal sequences' named entity 'EMCV' named entity 'transfected' named entity 'reporter gene' named entity 'protein' named entity 'picornavirus' named entity 'EMCV' named entity 'recombinants'

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