About: Rescue and evaluation of a recombinant PRRSV expressing porcine Interleukin-4

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About: Rescue and evaluation of a recombinant PRRSV expressing porcine Interleukin-4 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Rescue and evaluation of a recombinant PRRSV expressing porcine Interleukin-4
Creator	Wang, Yan Wang, Gang Cai, Xuehui Zhang, Chong Zhou, En-Min Ding, Peiyang Huang, Baicheng Wang, Chengbao Li, Qiongyi Li, Zhijun Li, Liangliang Syed, Shahid Wang, Xinglong Xue, Biyun
Source	PMC
abstract	BACKGROUND: The current vaccines for porcine reproductive and respiratory syndrome virus (PRRSV) have failed to provide broad protection against infection by various strains of PRRSV. Porcine Interleukin-4 (pIL-4) plays an important role in the regulation of the immune response and has been used previously as an immunological adjuvant. The objective of this study was to construct a recombinant PRRSV expressing pIL-4 and to evaluate the immune response of the recombinant virus in piglets. METHODS: The pIL-4 gene was inserted in the PRRSV (CH-1R strain) infectious clone by overlap PCR. Indirect immunofluorescence assay (IFA) and Western blotting were used to confirm the recombinant virus. The stability of the recombinant virus was assessed by DNA sequencing and IFA after 15 passages in vitro. Recombinant virus was injected into pigs and efficacy of immune protection was evaluated in comparison with the parental virus. RESULTS: The recombinant virus (CH-1R/pIL-4) was successfully rescued and shown to have similar growth kinetics as the parental virus. The recombinant virus was stable for 15 passages in cell culture. Pigs vaccinated with CH-1R/pIL-4 produced a similar humoral response to the response elicited by parental virus, but IL-4 level in the supernatant of PBMCs from pigs vaccinated with CH-1R/pIL-4 was significantly higher than the parent virus at 28 days post-immunization (DPI). Flow cytometric (FCM) analysis showed that the percentage of CD4(+)CD8(+) double positive T (DPT) cells in the CH-1R/pIL-4 vaccinated group was significantly higher than the parental virus at 3 and 7 Days Post-Challenge (DPC), and the IL-4 level in the blood significantly increased at 7 DPC. However, the viral load and histopathology did not show significant difference between the two groups. CONCLUSIONS: A recombinant PRRSV expressing porcine IL-4 was rescued and it remained genetically stable in vitro. The recombinant virus induced higher DPT ratios and IL-4 levels in the blood after HP-PRRSV challenge compared to the parental virus in piglets. However, it did not significantly improve protection efficacy of PRRSV vaccine.
has issue date	2015-11-14(xsd:dateTime)
bibo:doi	10.1186/s12985-015-0380-7
bibo:pmid	26573719
has license	cc-by
sha1sum (hex)	defb2baddf8a312718647fac6a0860606ff8907d
schema:url	https://doi.org/10.1186/s12985-015-0380-7
resource representing a document's title	Rescue and evaluation of a recombinant PRRSV expressing porcine Interleukin-4
has PubMed Central identifier	PMC4647277
has PubMed identifier	26573719
schema:publication	Virol J
resource representing a document's body	covid:defb2baddf8a312718647fac6a0860606ff8907d#body_text
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