About: Partial deletion in the spike endodomain of mouse hepatitis virus decreases the cytopathic effect but maintains foreign protein expression in infected cells

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About: Partial deletion in the spike endodomain of mouse hepatitis virus decreases the cytopathic effect but maintains foreign protein expression in infected cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Partial deletion in the spike endodomain of mouse hepatitis virus decreases the cytopathic effect but maintains foreign protein expression in infected cells
Creator	Wang, Yuyan Qu, Di Ye, Rong Lv, Jun Sun, Zhiping Yang, Jinhua
source	Elsevier; Medline; PMC
abstract	Mouse hepatitis virus (MHV) produces a series of subgenomic RNAs for viral protein expression. As a prototype coronavirus, MHV has been explored extensively and is often used to express foreign proteins. Previously, a 13-residue deletion in the MHV spike (S) protein endodomain was found to reduce syncytium formation dramatically while inhibiting virus replication slightly. In this study, the effects of the S mutation on MHV infectivity and foreign protein expression were further examined in rat or mouse L2, NIH/3T3 and Neuro-2a cells. The replacement of the MHV 2a/haemagglutinin-esterase gene with a membrane-anchored protein hook (HK) and replacement of gene 4 with EGFP did not change the adaptability and cytopathology of recombinant viruses in these cells. However, the cytopathic effect of the recombinants with the partial S deletion was reduced significantly in these cells. The replication and foreign protein expression of the S-mutated recombinants were found to be more efficient in L2 cells than in Neuro-2a and NIH/3T3 cells. Meanwhile, the distribution patterns of HK and EGFP expressed by the recombinant viruses were similar to those in cells transfected with a eukaryotic expression vector. These results suggest that the partial deletion in the S endodomain may increase the usefulness of MHV as a viral vector by attenuation and maintaining foreign protein expression.
has issue date	2010-12-28(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2010.12.018
bibo:pmid	21192978
has license	no-cc
sha1sum (hex)	de0ccd3f0c46405ba5d43bc0d565ee838e778d5a
schema:url	https://doi.org/10.1016/j.jviromet.2010.12.018
resource representing a document's title	Partial deletion in the spike endodomain of mouse hepatitis virus decreases the cytopathic effect but maintains foreign protein expression in infected cells
has PubMed Central identifier	PMC7112841
has PubMed identifier	21192978
schema:publication	J Virol Methods
resource representing a document's body	covid:de0ccd3f0c46405ba5d43bc0d565ee838e778d5a#body_text
is schema:about of	covid:arg/de0ccd3f0c46405ba5d43bc0d565ee838e778d5a named entity '3T3' named entity 'MHV' named entity 'coronavirus' named entity 'Mouse hepatitis virus' named entity 'protein expression' named entity 'virus replication' named entity 'recombinant' named entity 'viruses' named entity 'protein expression' named entity 'recombinants' named entity 'esterase' named entity 'protein expression' named entity 'protein' named entity 'C-terminal' named entity 'Neuro-2a' named entity 'MHV' named entity 'coronaviruses' named entity '3T3' named entity 'EGFP' named entity 'protein' named entity 'MHV' named entity 'expression vector' named entity 'hpi' named entity 'EGFP' named entity 'NIH' named entity 'infection' named entity 'immunofluorescence' named entity 'EGFP' named entity 'cloned' named entity 'MHV' named entity 'signal peptide' named entity 'viral replication' named entity 'proteins expressed' named entity 'Triton X-100' named entity 'Roche' named entity 'MHV' named entity 'MHV' named entity 'MHV' named entity 'transfected' named entity 'EGFP' named entity 'BSA' named entity 'RNA transcription' named entity 'coronaviruses' named entity 'cell lines' named entity 'MHV' named entity 'sodium bicarbonate' named entity 'poly A tail' named entity 'monoclonal antibody' named entity '3T3' named entity 'motif' named entity 'centrifugation' named entity 'virus' named entity 'Felis catus' named entity 'staining' named entity 'Neuro-2A' named entity 'Neuro-2a' named entity 'MHV' named entity 'MHV' named entity 'palmitoylation' named entity 'MOI' named entity 'viral replication' named entity '3T3' named entity 'haemagglutinin' named entity 'transfected' named entity 'secondary antibody' named entity 'EGFP' named entity 'EGFP' named entity 'plasmid' named entity 'confocal microscopy' named entity 'cell lines' named entity 'NaCl' named entity 'fusion peptide' named entity 'MHV' named entity 'MHV' named entity 'transfection'

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