About: Assessment of an Antibody-in-Lymphocyte Supernatant Assay for the Etiological Diagnosis of Pneumococcal Pneumonia in Children

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About: Assessment of an Antibody-in-Lymphocyte Supernatant Assay for the Etiological Diagnosis of Pneumococcal Pneumonia in Children Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Assessment of an Antibody-in-Lymphocyte Supernatant Assay for the Etiological Diagnosis of Pneumococcal Pneumonia in Children
Creator	Murdoch, David Carter, Michael Jones, Claire O'brien, Katherine Pollard, Andrew Russell, Clark Ong, David Kelly, Sarah Knight, Julian Voysey, Merryn Wahl, Brian Ansari, Imran Darton, Thomas Ekström, Nina Gautam, Madhav Gurung, Meeru Gurung, Pallavi Kandasamy, Rama Kelly, Dominic Khadka, Bibek Maharjan, Anju Prajapati, Krishna Rajkarnikar, Shristy Shah, Ganesh Shrestha, Shrijana Thorson, Stephen Dr, Murdoch Kg, Prajapati Kl, Brien Knight, Maharjan Maurin, Max Mc, Gautam Mj, Carter Tc, Darton Wahl B, O', Voysey
Source	Medline; PMC
abstract	New diagnostic tests for the etiology of childhood pneumonia are needed. We evaluated the antibody-in-lymphocyte supernatant (ALS) assay to detect immunoglobulin (Ig) G secretion from ex vivo peripheral blood mononuclear cell (PBMC) culture, as a potential diagnostic test for pneumococcal pneumonia. We enrolled 348 children with pneumonia admitted to Patan Hospital, Kathmandu, Nepal between December 2015 and September 2016. PBMCs sampled from participants were incubated for 48 h before harvesting of cell culture supernatant (ALS). We used a fluorescence-based multiplexed immunoassay to measure the concentration of IgG in ALS against five conserved pneumococcal protein antigens. Of children with pneumonia, 68 had a confirmed etiological diagnosis: 12 children had pneumococcal pneumonia (defined as blood or pleural fluid culture-confirmed; or plasma CRP concentration ≥60 mg/l and nasopharyngeal carriage of serotype 1 pneumococci), and 56 children had non-pneumococcal pneumonia. Children with non-pneumococcal pneumonia had either a bacterial pathogen isolated from blood (six children); or C-reactive protein <60 mg/l, absence of radiographic consolidation and detection of a pathogenic virus by multiplex PCR (respiratory syncytial virus, influenza viruses, or parainfluenza viruses; 23 children). Concentrations of ALS IgG to all five pneumococcal proteins were significantly higher in children with pneumococcal pneumonia than in children with non-pneumococcal pneumonia. The concentration of IgG in ALS to the best-performing antigen discriminated between children with pneumococcal and non-pneumococcal pneumonia with a sensitivity of 1.0 (95% CI 0.73–1.0), specificity of 0.66 (95% CI 0.52–0.78) and area under the receiver-operating characteristic curve (AUROCC) 0.85 (95% CI 0.75–0.94). Children with pneumococcal pneumonia were older than children with non-pneumococcal pneumonia (median 5.6 and 2.0 years, respectively, p < 0.001). When the analysis was limited to children ≥2 years of age, assay of IgG ALS to pneumococcal proteins was unable to discriminate between children with pneumococcal pneumonia and non-pneumococcal pneumonia (AUROCC 0.67, 95% CI 0.47–0.88). This method detected spontaneous secretion of IgG to pneumococcal protein antigens from cultured PBMCs. However, when stratified by age group, assay of IgG in ALS to pneumococcal proteins showed limited utility as a test to discriminate between pneumococcal and non-pneumococcal pneumonia in children.
has issue date	2020-01-17(xsd:dateTime)
bibo:doi	10.3389/fcimb.2019.00459
bibo:pmid	32039044
has license	cc-by
sha1sum (hex)	ddb8d76c619e669ff79a26da31a2cd99700df433
schema:url	https://doi.org/10.3389/fcimb.2019.00459
resource representing a document's title	Assessment of an Antibody-in-Lymphocyte Supernatant Assay for the Etiological Diagnosis of Pneumococcal Pneumonia in Children
has PubMed Central identifier	PMC6988833
has PubMed identifier	32039044
schema:publication	Front Cell Infect Microbiol
resource representing a document's body	covid:ddb8d76c619e669ff79a26da31a2cd99700df433#body_text
is schema:about of	named entity 'harvesting' named entity 'supernatant' named entity 'pneumonia' named entity 'pneumonia' named entity '2016' named entity 'peripheral blood mononuclear cell' named entity 'protein' named entity 'Nepal' named entity 'virus' named entity 'pneumococci' named entity 'pneumococcal pneumonia' named entity 'FLUID CULTURE' named entity 'PNEUMONIA' named entity 'nasopharyngeal' named entity 'pneumonia' named entity 'pneumococcal pneumonia' named entity 'defined' named entity 'IgG' named entity 'conserved' named entity 'sensitivity' named entity 'September' named entity 'assay' named entity 'pneumococcal pneumonia' named entity 'influenza' named entity 'respiratory syncytial virus' named entity 'concentration' named entity 'Children' named entity 'cell culture' named entity 'concentration' named entity 'blood' named entity 'isolated' named entity 'plasma' named entity 'influenza viruses' named entity 'PBMC' named entity 'multiplexed' named entity 'immunoassay' named entity 'antibody' named entity 'pathogenic virus' named entity 'peripheral blood mononuclear cell' named entity 'cell culture' named entity 'pneumococcal pneumonia' named entity 'C-reactive protein' named entity 'pneumonia' named entity 'supernatant' named entity 'etiology' named entity 'serotype' named entity 'Antibody'

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