About: Identification of Appropriate Reference Genes for qRT-PCR Analysis of Heat-Stressed Mammary Epithelial Cells in Riverine Buffaloes (Bubalus bubalis)

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About: Identification of Appropriate Reference Genes for qRT-PCR Analysis of Heat-Stressed Mammary Epithelial Cells in Riverine Buffaloes (Bubalus bubalis) Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Identification of Appropriate Reference Genes for qRT-PCR Analysis of Heat-Stressed Mammary Epithelial Cells in Riverine Buffaloes (Bubalus bubalis)
Creator	Jerath, Tanushri Kapila, Neha Kishore, Amit Kumar, Pawan Sharma, Ankita Sodhi, Monika Mohanty, A Mukesh, M
Source	PMC
abstract	Gene expression studies require appropriate normalization methods for proper evaluation of reference genes. To date, not many studies have been reported on the identification of suitable reference genes in buffaloes. The present study was undertaken to determine the panel of suitable reference genes in heat-stressed buffalo mammary epithelial cells (MECs). Briefly, MEC culture from buffalo mammary gland was exposed to 42 °C for one hour and subsequently allowed to recover at 37 °C for different time intervals (from 30 m to 48 h). Three different algorithms, geNorm, NormFinder, and BestKeeper softwares, were used to evaluate the stability of 16 potential reference genes from different functional classes. Our data identified RPL4, EEF1A1, and RPS23 genes to be the most appropriate reference genes that could be utilized for normalization of qPCR data in heat-stressed buffalo MECs.
has issue date	2013-01-28(xsd:dateTime)
bibo:doi	10.5402/2013/735053
bibo:pmid	25937980
has license	cc-by
sha1sum (hex)	dd40ad3eec9f455f6ab725f007eb32e852b3439c
schema:url	https://doi.org/10.5402/2013/735053
resource representing a document's title	Identification of Appropriate Reference Genes for qRT-PCR Analysis of Heat-Stressed Mammary Epithelial Cells in Riverine Buffaloes (Bubalus bubalis)
has PubMed Central identifier	PMC4393032
has PubMed identifier	25937980
schema:publication	ISRN Biotechnol
resource representing a document's body	covid:dd40ad3eec9f455f6ab725f007eb32e852b3439c#body_text
is schema:about of	named entity 'EPITHELIAL CELLS' named entity 'METHODS' named entity 'EVALUATE' named entity 'ONE HOUR' named entity 'APPROPRIATE' named entity 'PRESENT' named entity 'OUR' named entity 'IDENTIFIED' named entity 'ALLOWED' named entity 'STUDY' named entity 'DATE' named entity 'GENE EXPRESSION' named entity 'DATA' named entity 'MAMMARY' named entity 'SUITABLE' named entity 'RECOVER' named entity 'REFERENCE' named entity 'PCR ANALYSIS' named entity 'buffaloes' named entity 'reference' named entity 'qPCR' named entity 'QRT-PCR' named entity 'EPITHELIAL CELLS' named entity 'BUFFALOES' named entity 'NORMALIZATION' named entity 'DETERMINE' named entity 'BUFFALOES' named entity 'HEAT' named entity 'STRESSED' named entity 'STUDIES' named entity 'MAMMARY GLAND' named entity 'EEF1A1' named entity 'PROPER' named entity 'INTERVALS' named entity 'REFERENCE' named entity 'MAMMARY' named entity 'HEAT' named entity 'IDENTIFICATION' named entity 'GENES' named entity 'POTENTIAL' named entity 'REPORTED' named entity 'GENES' named entity 'HAVE' named entity 'EVALUATION' named entity 'RPS23' named entity 'PANEL' named entity 'STRESSED' named entity 'APPROPRIATE' named entity 'BUFFALO' named entity 'IDENTIFICATION' named entity 'EXPOSED TO' named entity 'ALGORITHMS' named entity 'STABILITY' named entity 'SOFTWARES' named entity 'COULD BE' named entity 'CLASSES' named entity 'FUNCTIONAL' named entity 'TIME' named entity 'MEC' named entity 'DIFFERENT' named entity 'CULTURE' named entity 'USED' named entity 'RPL4' named entity 'QPCR DATA' named entity 'MECS' covid:arg/dd40ad3eec9f455f6ab725f007eb32e852b3439c named entity 'panel' named entity 'buffalo' named entity 'reference' named entity 'proper' named entity 'Epithelial' named entity 'mammary epithelial cells' named entity 'mammary gland' named entity 'reference genes'

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