About: Single-cell longitudinal analysis of SARS-CoV-2 infection in human bronchial epithelial cells

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About: Single-cell longitudinal analysis of SARS-CoV-2 infection in human bronchial epithelial cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Single-cell longitudinal analysis of SARS-CoV-2 infection in human bronchial epithelial cells
Creator	Iwasaki, Akiko Alfajaro, Mia Montgomery, Ruth Pyle, Anna Foxman, Ellen Wang, Bao Wilen, Craig Eisenbarth, Stephanie Filler, Renata Gasque, Victor Horvath, Tamas Huston, Nicholas Ravindra, Neal Szigeti-Buck, Klara Van Dijk, David Wan, Han Wei, Jin Williams, Adam
source	BioRxiv; Medline; PMC
abstract	SARS-CoV-2, the causative agent of COVID-19, has resulted in more than 3,000,000 infections and 200,000 deaths. There are currently no approved drugs or vaccines for the treatment or prevention of COVID-19. Enhanced understanding of SARS-CoV-2 infection and pathogenesis is critical for the development of therapeutics. To reveal insight into viral replication, cell tropism, and host-viral interactions of SARS-CoV-2 we performed single-cell RNA sequencing of experimentally infected human bronchial epithelial cells (HBECs) in air-liquid interface cultures over a time-course. This revealed novel polyadenylated viral transcripts and highlighted ciliated cells as the major target of infection, which we confirmed by electron microscopy. Over the course of infection, cell tropism of SARS-CoV-2 expands to other epithelial cell types including basal and club cells. Infection induces cell intrinsic expression of type I and type III IFNs and IL6 but not IL1. This results in expression of interferon stimulated genes in both infected and bystander cells. Here, we have conducted an in-depth analysis of SARS-CoV-2 infection in HBECs and provide a detailed characterization of genes, cell types, and cell state changes associated with the infection.
has issue date	2020-05-07(xsd:dateTime)
bibo:doi	10.1101/2020.05.06.081695
bibo:pmid	32511382
has license	cc-by-nc
sha1sum (hex)	dd1dfdb0819b8dd54d05b9b4ff1fe8c895d1f11c
schema:url	https://doi.org/10.1101/2020.05.06.081695
resource representing a document's title	Single-cell longitudinal analysis of SARS-CoV-2 infection in human bronchial epithelial cells
has PubMed Central identifier	PMC7263511
has PubMed identifier	32511382
schema:publication	bioRxiv
resource representing a document's body	covid:dd1dfdb0819b8dd54d05b9b4ff1fe8c895d1f11c#body_text
is schema:about of	named entity 'IL1' named entity 'reveal' named entity 'single-cell' named entity 'detailed' named entity 'human' named entity 'SARS-CoV-2' named entity 'VACCINES' named entity 'BRONCHIAL' named entity 'BUT' named entity 'THERAPEUTICS' named entity 'POLYADENYLATED' named entity 'CAUSATIVE AGENT' named entity 'RESULTS' named entity 'INFECTION' named entity 'RNA SEQUENCING' named entity 'CELL' named entity 'HAVE' named entity 'interactions' named entity 'causative' named entity 'electron microscopy' named entity 'RNA' named entity 'infection' named entity 'infected' named entity 'COVID-19' named entity 'pathogenesis' named entity 'prevention' named entity 'SARS-CoV-2' named entity 'infection' named entity 'SARS-CoV-2' named entity 'infection' named entity 'infection' named entity 'COVID-19' named entity 'COVID' named entity 'causative agent' named entity 'approved drugs' named entity 'infection' named entity 'viral replication' named entity 'SARS-CoV-2' named entity 'bronchial epithelial cells' named entity 'SARS-CoV-2' named entity 'Life Technologies' named entity 'Single-cell transcriptomics' named entity 'ACE2' named entity 'RNA' named entity 'preprint' named entity 'infection' named entity 'viruses' named entity 'infection' named entity 'RNAs' named entity 'morbidity' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'cell tropism' named entity 'COVID-19' named entity 'viral entry' named entity 'non-structural protein' named entity 'epithelium' named entity 'viral genome' named entity 'qPCR' named entity 'PBS' named entity 'poly-A tails' named entity 'coronavirus' named entity 'cell types' named entity '10x Genomics'

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