About: Pooling RT-PCR test of SARS-CoV-2 for large cohort of 'healthy' and infection-suspected patients: A prospective and consecutive study on 1,000 individuals

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About: Pooling RT-PCR test of SARS-CoV-2 for large cohort of 'healthy' and infection-suspected patients: A prospective and consecutive study on 1,000 individuals Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Pooling RT-PCR test of SARS-CoV-2 for large cohort of 'healthy' and infection-suspected patients: A prospective and consecutive study on 1,000 individuals
Creator	Omata, Masao Hirotsu, Yosuke Mochizuki, Hitoshi Amemiya, Kenji Hosaka, Kazuhiro Maejima, Makoto Nagakubo, Yuki Shibusawa, Masahiro Sueki, Hitomi Hayakawa, Miyoko
source	MedRxiv
abstract	Background SARS-CoV-2 testing reagents are expected to become in short supply worldwide. However, little is unknown whether the pooling strategy detects SARS-CoV-2 with accuracy. Method To validate the feasibility of pooling samples, serial dilution analysis and spike-in experiment were conducted using synthetic DNA and nucleic acids extracted from SARS-CoV-2 positive and negative patients. Furthermore, we studied a total of 1,000 individuals, who were 667 'healthy' (195 healthcare workers and 472 hospitalized patients with other disorders than COVID-19 infection) individuals and 333 infection-suspected patients with cough and fever, were tested. Results Serial dilution analysis showed the limit of detection of around 10-100 copies according to National Institute of Infectious Diseases, Japan. Spike-in experiment demonstrated RT-qPCR detect positive signal in pooling samples of SARS-CoV-2 negative and positive patient at the 5-, 10-, 20-fold dilution. By screening with pooling strategy by the end of April, 2020, there are 12 COVID-19 patients in 333 infection suspected patients (3.6%) and zero in 667 'healthy'. We obtained these results with total running 538 times (instead of 1,000 times) by pooling strategy. Conclusion Pooling samples is feasible for saving test reagents and detecting SARS-CoV-2 in clinical setting to prevent the spread of the virus and nosocomial transmission.
has issue date	2020-05-06(xsd:dateTime)
bibo:doi	10.1101/2020.05.04.20088146
has license	medrxiv
sha1sum (hex)	dcfeebcf556f78c490de4ec0700eb6c36da796e1
schema:url	https://doi.org/10.1101/2020.05.04.20088146
resource representing a document's title	Pooling RT-PCR test of SARS-CoV-2 for large cohort of 'healthy' and infection-suspected patients: A prospective and consecutive study on 1,000 individuals
resource representing a document's body	covid:dcfeebcf556f78c490de4ec0700eb6c36da796e1#body_text
is schema:about of	named entity 'ACCURACY' named entity 'testing' named entity 'SARS-CoV-2' named entity 'short supply' named entity 'SARS-CoV-2' named entity 'Copan' named entity 'short supply' named entity 'medRxiv' named entity 'May 6' named entity 'Declaration of Helsinki' named entity 'cotton swab' named entity 'infection' named entity 'Murrieta' named entity 'preprint' named entity 'preprint' named entity 'Tokyo' named entity 'Institutional Review Board' named entity 'nucleic acid' named entity 'medRxiv' named entity 'preprint' named entity 'nasopharyngeal swabs' named entity 'nasopharyngeal swabs' named entity 'preprint' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'infection' named entity 'POOLING' named entity 'STRATEGY' covid:arg/dcfeebcf556f78c490de4ec0700eb6c36da796e1 named entity 'TESTING' named entity 'RT-PCR test' named entity 'SARS-COV-2' named entity 'LARGE' named entity 'RT-PCR' named entity 'SARS-COV-2' named entity 'SUSPECTED' named entity 'TEST' named entity 'SARS-CoV-2' named entity 'reagents' named entity 'expected' named entity 'prospective' named entity 'strategy' named entity 'large' named entity 'REAGENTS' named entity 'UNKNOWN' named entity 'LITTLE' named entity 'EXPECTED' named entity 'detects' named entity 'short' named entity 'RT-PCR' named entity 'patients' named entity 'individuals' named entity 'SARS-CoV-2' named entity 'PROSPECTIVE' named entity 'SARS-CoV-2' named entity 'SHORT' named entity 'cohort' named entity 'SUPPLY' named entity 'WORLDWIDE' named entity 'COHORT' named entity 'POOLING' named entity 'STUDY' named entity 'INFECTION' named entity 'CONSECUTIVE' named entity 'PATIENTS' named entity 'INDIVIDUALS'
is wasQuotedFrom of	covid:arg/dcfeebcf556f78c490de4ec0700eb6c36da796e1/0
is part of of	Background SARS-CoV-2 testing reagents are expected to become in short supply worldwide. However, little is unknown whether the pooling strategy detects SARS-CoV-2 with accuracy. Method To validate the feasibility of pooling samples, serial dilution analysis and spike-in experiment were conducted using synthetic DNA and nucleic acids extracted from SARS-CoV-2 positive and negative patients. Furthermore, we studied a total of 1,000 individuals, who were 667 'healthy' (195 healthcare workers and 472 hospitalized patients with other disorders than COVID-19 infection) individuals and 333 infection-suspected patients with cough and fever, were tested. Results Serial dilution analysis showed the limit of detection of around 10-100 copies according to National Institute of Infectious Diseases, Japan. Spike-in experiment demonstrated RT-qPCR detect positive signal in pooling samples of SARS-CoV-2 negative and positive patient at the 5-, 10-, 20-fold dilution. By screening with pooling strategy by the end of April, 2020, there are 12 COVID-19 patients in 333 infection suspected patients (3.6%) and zero in 667 'healthy'. We obtained these results with total running 538 times (instead of 1,000 times) by pooling strategy. Conclusion Pooling samples is feasible for saving test reagents and detecting SARS-CoV-2 in clinical setting to prevent the spread of the virus and nosocomial transmission. covid:dcfeebcf556f78c490de4ec0700eb6c36da796e1#body_text Pooling RT-PCR test of SARS-CoV-2 for large cohort of 'healthy' and infection-suspected patients: A prospective and consecutive study on 1,000 individuals

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