About: Genetic variations on 31 and 450 residues of influenza A nucleoprotein affect viral replication and translation

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About: Genetic variations on 31 and 450 residues of influenza A nucleoprotein affect viral replication and translation Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Genetic variations on 31 and 450 residues of influenza A nucleoprotein affect viral replication and translation
Creator	Barr, Ian Hurt, Aeron Wang, Jen-Ren Huang, Sheng-Wen Shih, Shin-Ru Tsai, Huey-Pin Lee, Yang Hsu, Yin-Mei Hung, Su-Jhen Yi, Leo
Source	PMC
abstract	BACKGROUND: Influenza A viruses cause epidemics/severe pandemics that pose a great global health threat. Among eight viral RNA segments, the multiple functions of nucleoprotein (NP) play important roles in viral replication and transcription. METHODS: To understand how NP contributes to the virus evolution, we analyzed the NP gene of H3N2 viruses in Taiwan and 14,220 NP sequences collected from Influenza Research Database. The identified genetic variations were further analyzed by mini-genome assay, virus growth assay, viral RNA and protein expression as well as ferret model to analyze their impacts on viral replication properties. RESULTS: The NP genetic analysis by Taiwan and global sequences showed similar evolution pattern that the NP backbones changed through time accompanied with specific residue substitutions from 1999 to 2018. Other than the conserved residues, fifteen sporadic substitutions were observed in which the 31R, 377G and 450S showed higher frequency. We found 31R and 450S decreased polymerase activity while the dominant residues (31 K and 450G) had higher activity. The 31 K and 450G showed better viral translation and replication in vitro and in vivo. CONCLUSIONS: These findings indicated variations identified in evolution have roles in modulating viral replication in vitro and in vivo. This study demonstrates that the interaction between variations of NP during virus evolution deserves future attention.
has issue date	2020-01-06(xsd:dateTime)
bibo:doi	10.1186/s12929-019-0612-z
bibo:pmid	31906961
has license	cc-by
sha1sum (hex)	db11d170784c1b66f0b27221156019953597e56a
schema:url	https://doi.org/10.1186/s12929-019-0612-z
resource representing a document's title	Genetic variations on 31 and 450 residues of influenza A nucleoprotein affect viral replication and translation
has PubMed Central identifier	PMC6943894
has PubMed identifier	31906961
schema:publication	J Biomed Sci
resource representing a document's body	covid:db11d170784c1b66f0b27221156019953597e56a#body_text
is schema:about of	named entity 'segments' named entity 'translation' named entity 'residues' named entity 'variations' named entity 'VIRAL RNA' named entity 'EPIDEMICS' named entity 'PROPERTIES' named entity 'NUCLEOPROTEIN' named entity 'IMPACTS' named entity 'collected' named entity 'transcription' named entity 'protein' named entity 'model' named entity 'Methods' named entity 'pose' named entity 'viral replication' named entity 'pandemics' named entity 'Influenza A' named entity 'viral RNA' named entity 'Taiwan' named entity 'RNP' named entity 'amino acid sequences' named entity 'MDCK' named entity 'nucleotide sequences' named entity 'amino acid sequence' named entity 'virus replication' named entity 'culture medium' named entity 'gene' named entity 'Influenza' named entity 'PCR' named entity 'crystal violet' named entity 'ferret' named entity 'RSS' named entity 'reverse genetics' named entity 'epidemiology' named entity 'NCKUH' named entity '450S' named entity 'intranasally' named entity 'ribonucleoprotein' named entity 'virus evolution' named entity 'β-actin' named entity 'plasmids' named entity 'RNAs' named entity 'genome' named entity 'luciferase' named entity 'seasonal influenza' named entity 'tissue culture' named entity 'influenza viruses' named entity 'H1N1' named entity 'Plaque assay' named entity 'DMEM' named entity 'significant difference' named entity 'polymerase' named entity 'gene' named entity 'serially diluted' named entity 'Taiwan' named entity 'viral replication' named entity 'genetic variations' named entity 'transfected' named entity 'viruses' named entity 'Taiwan' named entity 'evolutionary mechanisms' named entity 'electronic identification' named entity 'amino acid sequence' named entity 'gene' named entity 'amino acid sequences' named entity 'Epistasis' named entity 'primers' named entity 'primer' named entity 'viruses' named entity 'quantitative PCR' named entity 'Centers for Disease Control of Taiwan'

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