About: Development and Validation of a S1 Protein-Based ELISA for the Specific Detection of Antibodies against Equine Coronavirus

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Development and Validation of a S1 Protein-Based ELISA for the Specific Detection of Antibodies against Equine Coronavirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development and Validation of a S1 Protein-Based ELISA for the Specific Detection of Antibodies against Equine Coronavirus
Creator	Pusterla, Nicola Barnum, Samantha Bosch, Berend-Jan Egberink, Herman Schuurman, Nancy Smits, Constance Zhao, Shan Van Kuppeveld, Frank Van Maanen, Kees
Source	Medline; PMC
abstract	Equine coronavirus (ECoV) is considered to be involved in enteric diseases in foals. Recently, several outbreaks of ECoV infection have also been reported in adult horses from the USA, France and Japan. Epidemiological studies of ECoV infection are still limited, and the seroprevalence of ECoV infection in Europe is unknown. In this study, an indirect enzyme-linked immunosorbent assay (ELISA) method utilizing ECoV spike S1 protein was developed in two formats, and further validated by analyzing 27 paired serum samples (acute and convalescent sera) from horses involved in an ECoV outbreak and 1084 sera of horses with unknown ECoV exposure. Both formats showed high diagnostic accuracy compared to virus neutralization (VN) assay. Receiver-operating characteristic (ROC) analyses were performed to determine the best cut-off values for both ELISA formats, assuming a test specificity of 99%. Employing the developed ELISA method, we detected seroconversion in 70.4% of horses from an ECoV outbreak. Among the 1084 horse sera, seropositivity varied from 25.9% (young horses) to 82.8% (adult horses) in Dutch horse populations. Further, sera of Icelandic horses were included in this study and a significant number of sera (62%) were found to be positive. Overall, the results demonstrated that the ECoV S1-based ELISA has reliable diagnostic performance compared to the VN assay and is a useful assay to support seroconversion in horses involved with ECoV outbreaks and to estimate ECoV seroprevalence in populations of horses.
has issue date	2019-11-30(xsd:dateTime)
bibo:doi	10.3390/v11121109
bibo:pmid	31801275
has license	cc-by
sha1sum (hex)	dafc5a833630d362e8ac97ce094a669204ef62e5
schema:url	https://doi.org/10.3390/v11121109
resource representing a document's title	Development and Validation of a S1 Protein-Based ELISA for the Specific Detection of Antibodies against Equine Coronavirus
has PubMed Central identifier	PMC6950238
has PubMed identifier	31801275
schema:publication	Viruses
resource representing a document's body	covid:dafc5a833630d362e8ac97ce094a669204ef62e5#body_text
is schema:about of	named entity 'DETECTION' named entity 'limited' named entity 'ROC' named entity 'horses' named entity 'populations' named entity 'specificity' named entity 'infection' named entity 'acute' named entity 'FORMATS' named entity 'TEST' named entity 'EQUINE CORONAVIRUS' named entity 'DETERMINE' named entity 'CONVALESCENT' named entity 'INFECTION' named entity 'UNKNOWN' named entity 'PROTEIN' named entity 'SPECIFIC' named entity 'DEVELOPMENT' named entity 'NEUTRALIZATION ' named entity 'SPECIFICITY' named entity 'BASED' named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAY' named entity 'ASSAY' named entity 'SERUM' named entity 'VALUES' named entity 'JAPAN' named entity 'POSITIVE' named entity 'BEST' named entity 'METHOD' named entity 'ADULT' named entity 'RECEIVER' named entity 'DIAGNOSTIC ACCURACY' named entity 'OVERALL' named entity 'LIMITED' named entity 'PERFORMANCE' named entity 'DETECTED' named entity 'ESTIMATE' named entity 'ELISA' named entity 'ANTIBODIES' named entity 'SEROPREVALENCE' named entity 'PERFORMED' named entity 'EXPOSURE' named entity 'EPIDEMIOLOGICAL STUDIES' named entity 'HAVE' named entity 'USEFUL' named entity 'SEROPOSITIVITY' named entity 'DEMONSTRATED' named entity 'REPORTED' named entity 'ICELANDIC' named entity 'RELIABLE' named entity 'HORSES' named entity 'USA' named entity 'OUTBREAK' named entity 'STUDY' named entity 'EMPLOYING' named entity 'BASED' named entity 'SPIKE' named entity 'DUTCH' named entity 'VALIDATED' named entity 'INDIRECT' named entity 'HORSE' named entity 'ELISA' named entity 'SIGNIFICANT' named entity 'CUT' named entity 'DIAGNOSTIC' named entity 'ANALYZING' named entity 'RECENTLY' named entity 'FRANCE' named entity 'SERA' named entity 'SEROCONVERSION' named entity 'VIRUS NEUTRALIZATION' named entity 'EUROPE' named entity 'PAIRED'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software