About: Efficient Replication of the Novel Human Betacoronavirus EMC on Primary Human Epithelium Highlights Its Zoonotic Potential

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About: Efficient Replication of the Novel Human Betacoronavirus EMC on Primary Human Epithelium Highlights Its Zoonotic Potential Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Efficient Replication of the Novel Human Betacoronavirus EMC on Primary Human Epithelium Highlights Its Zoonotic Potential
Creator	Fouchier, Ron Thiel, Volker Dijkman, Ronald Drosten, Christian Kindler, Eveline Muth, Doreen Hartmann, Rune Geffers, Robert Rodriguez, Regulo Müller, Marcel Buchmeier, Michael Hamming, Ole Jónsdóttir, Hulda
source	Medline; PMC
abstract	The recent emergence of a novel human coronavirus (HCoV-EMC) in the Middle East raised considerable concerns, as it is associated with severe acute pneumonia, renal failure, and fatal outcome and thus resembles the clinical presentation of severe acute respiratory syndrome (SARS) observed in 2002 and 2003. Like SARS-CoV, HCoV-EMC is of zoonotic origin and closely related to bat coronaviruses. The human airway epithelium (HAE) represents the entry point and primary target tissue for respiratory viruses and is highly relevant for assessing the zoonotic potential of emerging respiratory viruses, such as HCoV-EMC. Here, we show that pseudostratified HAE cultures derived from different donors are highly permissive to HCoV-EMC infection, and by using reverse transcription (RT)-PCR and RNAseq data, we experimentally determined the identity of seven HCoV-EMC subgenomic mRNAs. Although the HAE cells were readily responsive to type I and type III interferon (IFN), we observed neither a pronounced inflammatory cytokine nor any detectable IFN responses following HCoV-EMC, SARS-CoV, or HCoV-229E infection, suggesting that innate immune evasion mechanisms and putative IFN antagonists of HCoV-EMC are operational in the new host. Importantly, however, we demonstrate that both type I and type III IFN can efficiently reduce HCoV-EMC replication in HAE cultures, providing a possible treatment option in cases of suspected HCoV-EMC infection.
has issue date	2013-02-19(xsd:dateTime)
bibo:doi	10.1128/mbio.00611-12
bibo:pmid	23422412
has license	cc-by-nc-sa
sha1sum (hex)	da700649fef6382fec2ae238b91ffdbbc0e2c301
schema:url	https://doi.org/10.1128/mbio.00611-12
resource representing a document's title	Efficient Replication of the Novel Human Betacoronavirus EMC on Primary Human Epithelium Highlights Its Zoonotic Potential
has PubMed Central identifier	PMC3573664
has PubMed identifier	23422412
schema:publication	mBio
resource representing a document's body	covid:da700649fef6382fec2ae238b91ffdbbc0e2c301#body_text
is schema:about of	named entity 'EFFICIENT' named entity 'HUMAN CORONAVIRUS' named entity 'USING' named entity 'CULTURES' named entity 'RESPIRATORY VIRUSES' named entity 'EXPERIMENTALLY DETERMINED' named entity 'BAT' named entity 'CORONAVIRUSES' named entity 'PRIMARY' named entity 'CLINICAL PRESENTATION' named entity 'DERIVED' named entity 'TISSUE' named entity 'SEVEN' named entity 'SARS-COV' named entity 'epithelium' named entity 'reverse transcription' named entity 'EMC' named entity 'Efficient' named entity 'POTENTIAL' covid:arg/da700649fef6382fec2ae238b91ffdbbc0e2c301 named entity 'ZOONOTIC' named entity 'RENAL FAILURE' named entity 'DIFFERENT' named entity 'ENTRY' named entity 'HERE' named entity 'RAISED' named entity 'ZOONOTIC' named entity 'REPLICATION' named entity 'SEVERE' named entity 'HIGHLY' named entity 'DONORS' named entity 'NOVEL' named entity 'BETACORONAVIRUS' named entity 'EPITHELIUM' named entity 'HUMAN' named entity 'ITS' named entity 'POTENTIAL' named entity 'EMERGENCE' named entity 'OBSERVED' named entity 'HAE' named entity 'RNASEQ' named entity 'ACUTE PNEUMONIA' named entity 'RELATED' named entity 'MIDDLE EAST' named entity 'INFECTION' named entity 'NOVEL' named entity 'AIRWAY EPITHELIUM' named entity 'PRIMARY' named entity 'PSEUDOSTRATIFIED' named entity 'HUMAN' named entity 'DATA' named entity 'ASSESSING' named entity 'PCR' named entity 'RELEVANT' named entity 'FATAL OUTCOME' named entity 'EMC' named entity 'ASSOCIATED WITH' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'TARGET' named entity 'CONSIDERABLE' named entity 'REVERSE TRANSCRIPTION' named entity 'IDENTITY' named entity 'RECENT' named entity 'ORIGIN' named entity 'LIKE' named entity 'POINT' named entity 'CONCERNS' named entity 'EMC' named entity 'infection'

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