About: MAP-Kinase Regulated Cytosolic Phospholipase A2 Activity Is Essential for Production of Infectious Hepatitis C Virus Particles

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About: MAP-Kinase Regulated Cytosolic Phospholipase A2 Activity Is Essential for Production of Infectious Hepatitis C Virus Particles Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	MAP-Kinase Regulated Cytosolic Phospholipase A2 Activity Is Essential for Production of Infectious Hepatitis C Virus Particles
Creator	Bartenschlager, Ralf Pietschmann, Thomas Haid, Sibylle Kaderali, Lars Fischl, Wolfgang Bankwitz, Dorothea Frentzen, Anne Gentzsch, Juliane Hueging, Kathrin Menzel, Nicolas
Source	Medline; PMC
abstract	Hepatitis C virus (HCV) has infected around 160 million individuals. Current therapies have limited efficacy and are fraught with side effects. To identify cellular HCV dependency factors, possible therapeutic targets, we manipulated signaling cascades with pathway-specific inhibitors. Using this approach we identified the MAPK/ERK regulated, cytosolic, calcium-dependent, group IVA phospholipase A2 (PLA2G4A) as a novel HCV dependency factor. Inhibition of PLA2G4A activity reduced core protein abundance at lipid droplets, core envelopment and secretion of particles. Moreover, released particles displayed aberrant protein composition and were 100-fold less infectious. Exogenous addition of arachidonic acid, the cleavage product of PLA2G4A-catalyzed lipolysis, but not other related poly-unsaturated fatty acids restored infectivity. Strikingly, production of infectious Dengue virus, a relative of HCV, was also dependent on PLA2G4A. These results highlight previously unrecognized parallels in the assembly pathways of these human pathogens, and define PLA2G4A-dependent lipolysis as crucial prerequisite for production of highly infectious viral progeny.
has issue date	2012-07-26(xsd:dateTime)
bibo:doi	10.1371/journal.ppat.1002829
bibo:pmid	22911431
has license	cc-by
sha1sum (hex)	da3a5e823cc89f0767743267672c3f9f719c9bd1
schema:url	https://doi.org/10.1371/journal.ppat.1002829
resource representing a document's title	MAP-Kinase Regulated Cytosolic Phospholipase A2 Activity Is Essential for Production of Infectious Hepatitis C Virus Particles
has PubMed Central identifier	PMC3406102
has PubMed identifier	22911431
schema:publication	PLoS Pathog
resource representing a document's body	covid:da3a5e823cc89f0767743267672c3f9f719c9bd1#body_text
is schema:about of	named entity 'protein' named entity 'reduced' named entity 'parallels' named entity 'Hepatitis C Virus' named entity 'Regulated' named entity 'HCV' named entity 'HCV' named entity 'progeny' named entity 'droplets' named entity 'MAPK/ERK' named entity 'HCV' named entity 'signaling cascades' named entity 'Exogenous' named entity 'infectivity' named entity 'PLA2G4A' named entity 'HCV' named entity 'therapeutic targets' named entity 'human pathogens' named entity 'Hepatitis C virus' named entity 'lipolysis' named entity 'NDGA' named entity 'HCV' named entity 'DENV' named entity 'serially diluted' named entity 'virus' named entity 'transfected' named entity 'HCV' named entity 'triple-bonded' named entity 'phospholipase' named entity 'Western blot' named entity 'RNA replication' named entity 'cPLA2' named entity 'HCV' named entity 'HCV' named entity 'MAPK/ERK pathway' named entity 'PLA2G4A' named entity 'Glycine' named entity 'PLA2G4A' named entity 'HCV' named entity 'Huh-7' named entity 'Adipose differentiation-related protein' named entity 'PMSF' named entity 'HCV' named entity 'nucleus' named entity 'human genome' named entity 'enveloped viruses' named entity 'NS5A' named entity 'PLA2G4A' named entity 'Statistical significance' named entity 'signaling cascades' named entity 'luciferase' named entity 'protein' named entity 'RNA' named entity 'core protein' named entity 'RNA' named entity 'virus' named entity 'cleavage' named entity 'lipid droplet' named entity 'GFP' named entity 'genotype' named entity 'enzyme' named entity 'U0126' named entity 'transfected' named entity 'ERK' named entity 'metabolism' named entity 'RNA' named entity 'ApoE' named entity 'Cell Signaling' named entity 'Life Technologies' named entity 'polyprotein' named entity 'intracellular' named entity 'Huh-7'

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