About: Genotyping of 30 kinds of cutaneous human papillomaviruses by a multiplex microfluidic loop-mediated isothermal amplification and visual detection method

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About: Genotyping of 30 kinds of cutaneous human papillomaviruses by a multiplex microfluidic loop-mediated isothermal amplification and visual detection method Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Genotyping of 30 kinds of cutaneous human papillomaviruses by a multiplex microfluidic loop-mediated isothermal amplification and visual detection method
Creator	Wang, Zhuo Gao, Xing-Hua Mao, Rui Wang, Yining Chen, Hong-Duo Du, Yu-Guang Qi, Rui-Qun Sun, Yu-Zhe
Source	Medline; PMC
abstract	BACKGROUND: Human papillomaviruses (HPVs), a group of non-enveloped small viruses with double-stranded circular DNA which lead to multiple skin diseases such as benign warts, are commonly seen in clinics. The current HPV detection systems aim mainly at mucosal HPVs, however, an efficient clinical approach for cutaneous HPVs detection is lacking. OBJECTIVES: To establish a rapid detection system for cutaneous HPVs using a colorimetric loop-mediated isothermal amplification (LAMP) with hydroxynaphthol blue (HNB) dye in combination with microfluidic technology. METHODS: L1 DNA sequences of the 30 cutaneous HPVs were chemically synthesized, and LAMP primers against L1 DNA were designed with use of an online LAMP designing tool. Isothermal amplification was performed with use of a water bath and the amplification results were inspected with the naked eye. Using PCR sequencing as a control method, the specificity and sensitivity of the new detection system were obtained by detecting clinical samples. RESULTS: The lower detection limit of the LAMP assay was 10(7) viral DNA copies/μl when tested on synthesized L1 DNA sequences, which was better than the conventional PCR. Compared to PCR sequencing, the sensitivity of HPV27, HPV2, HPV1, HPV57, HPV3, HPV4, HPV7 and HPV75 genotypes detections were 100%, whereas the specificity was 34.55, 45.12, 95.83, 98.59 and 97.62% respectively, when tested on clinical samples. CONCLUSIONS: The new cutaneous type HPV detection system is characterized by both a good sensitivity and specificity compared to conventional methods.
has issue date	2020-07-09(xsd:dateTime)
bibo:doi	10.1186/s12985-020-01373-3
bibo:pmid	32646520
has license	cc-by
sha1sum (hex)	d9864bf295387868e20be121ab711fcc58c95579
schema:url	https://doi.org/10.1186/s12985-020-01373-3
resource representing a document's title	Genotyping of 30 kinds of cutaneous human papillomaviruses by a multiplex microfluidic loop-mediated isothermal amplification and visual detection method
has PubMed Central identifier	PMC7345449
has PubMed identifier	32646520
schema:publication	Virol J
resource representing a document's body	covid:d9864bf295387868e20be121ab711fcc58c95579#body_text
is schema:about of	named entity 'warts' named entity 'microfluidic' named entity 'SYSTEMS' named entity 'CURRENT' named entity 'CLINICS' named entity 'DETECTION' named entity 'DNA' named entity 'cutaneous' named entity 'Genotyping' named entity 'HPV' named entity 'skin diseases' named entity 'detection systems' named entity 'HPVs' named entity 'double-stranded' named entity 'non-enveloped' named entity 'Human papillomaviruses' named entity 'cutaneous' named entity 'human papillomaviruses' named entity 'Genotyping' named entity 'HPV' named entity 'Eluent' named entity 'HPVs' named entity 'PCR reaction' named entity 'UV-Vis' named entity 'DNA sample' named entity 'primers' named entity 'cross-reaction' named entity 'plasmid' named entity 'cutaneous' named entity 'HPV' named entity 'HPV' named entity 'primers' named entity 'PCR' named entity 'primers' named entity 'cross-reactions' named entity 'cutaneous' named entity 'HPV' named entity 'plasmid' named entity 'plantar' named entity 'gene sequences' named entity 'HPV' named entity 'microliters' named entity 'primers' named entity 'cross-reactions' named entity 'HPV' named entity 'HPV' named entity 'HPVs' named entity 'Scurf' named entity 'HPVs' named entity 'HPV' named entity 'cutaneous' named entity 'HPV' named entity 'primers' named entity 'cross reactivity' named entity 'plasmid' named entity 'cervical cancer' named entity 'HPV' named entity 'flat warts' named entity 'universal primers' named entity 'HPV' named entity 'infection rate' named entity 'cutaneous' named entity 'risk group' named entity 'PCR' named entity 'HPVs' named entity 'primers' named entity 'HPV infections' named entity 'HPV' named entity 'HPV' named entity 'genus' named entity 'gene' named entity 'plasmid' named entity 'PCR' named entity 'RNase'

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