About: TRIM62 From Chicken as a Negative Regulator of Reticuloendotheliosis Virus Replication

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About: TRIM62 From Chicken as a Negative Regulator of Reticuloendotheliosis Virus Replication Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	TRIM62 From Chicken as a Negative Regulator of Reticuloendotheliosis Virus Replication
Creator	Wang, Guihua Yang, Jie Liu, Baoming Li, Ling Subbiah, Madhuri Bi, Jianmin Zhang, Lingjuan Wiratsudakul, Anuwat Li, L Cheng, Ziqiang Niu, Dongyan
Source	PMC
abstract	Emerging evidence suggests that the tripartite motif containing 62 (TRIM62), a member of the TRIM family, plays an important role in antiviral processes. The objective of the study was to explore the role of TRIM62 in reticuloendotheliosis virus (REV) infection and its potential molecular mechanism. We first demonstrated that the REV infection affected the TRIM62 expression first upregulated and then downregulated in CEF cells. Next, we evaluated the effect of TRIM62 on viral replication. Overexpression of TRIM62 decreased REV replication. On the contrary, silencing of endogenously expressed TRIM62 increased viral replication. Then, to explore the necessity of domains in TRIM62's negative regulation on viral replication, we transfected CEF cells with TRIM62 domain deletion mutants. Deletion domain partially abolished TRIM62's antiviral activity. The effect of SPRY domain deletion was the highest and that of coiled-coil was the lowest. Further, we identified 18 proteins that coimmunoprecipitated and interacted with TRIM62 by immunocoprecipitation and mass spectrometry analysis. Strikingly, among which, both Ras-related protein Rab-5b (RAB5B) and Arp2/3 complex 34-kDa subunit (ARPC2) were involved in actin cytoskeletal pathway. Altogether, these results strongly suggest that chicken TRIM62 provides host defense against viral infection, and all domains are required for its action. RAB5B and ARPC2 may play important roles in its negative regulation processes.
has issue date	2020-04-03(xsd:dateTime)
bibo:doi	10.3389/fvets.2020.00152
has license	cc-by
sha1sum (hex)	d93c41ec19c3c8ee0f70f131ef06fd034a0db9ea
schema:url	https://doi.org/10.3389/fvets.2020.00152
resource representing a document's title	TRIM62 From Chicken as a Negative Regulator of Reticuloendotheliosis Virus Replication
has PubMed Central identifier	PMC7146716
schema:publication	Front Vet Sci
resource representing a document's body	covid:d93c41ec19c3c8ee0f70f131ef06fd034a0db9ea#body_text
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