About: Further Evidence for Bats as the Evolutionary Source of Middle East Respiratory Syndrome Coronavirus

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About: Further Evidence for Bats as the Evolutionary Source of Middle East Respiratory Syndrome Coronavirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Further Evidence for Bats as the Evolutionary Source of Middle East Respiratory Syndrome Coronavirus
Creator	Schultz-Cherry, Stacey Anthony, S Baric, R Bl, Yount Byarugaba, D Ck, Johnson Cranfield, M Debbink, K Dinnon, K Dk, Byarugaba Gilardi, K Goldstein, T Hicks, A Johnson, C Jude, St Kh, Dinnon Liang, E Liang, Navarrete-Macias I Lipkin, W Mazet, J Mbabazi, R Menachery, V Navarrete-Macias, I Petrosov, A Randell, S Scobey, T Sh, Randell Sj, Anthony Ssebide, B Vd, Menachery Wells, H Yount, B
Source	Medline; PMC
abstract	The evolutionary origins of Middle East respiratory syndrome (MERS) coronavirus (MERS-CoV) are unknown. Current evidence suggests that insectivorous bats are likely to be the original source, as several 2c CoVs have been described from various species in the family Vespertilionidae. Here, we describe a MERS-like CoV identified from a Pipistrellus cf. hesperidus bat sampled in Uganda (strain PREDICT/PDF-2180), further supporting the hypothesis that bats are the evolutionary source of MERS-CoV. Phylogenetic analysis showed that PREDICT/PDF-2180 is closely related to MERS-CoV across much of its genome, consistent with a common ancestry; however, the spike protein was highly divergent (46% amino acid identity), suggesting that the two viruses may have different receptor binding properties. Indeed, several amino acid substitutions were identified in key binding residues that were predicted to block PREDICT/PDF-2180 from attaching to the MERS-CoV DPP4 receptor. To experimentally test this hypothesis, an infectious MERS-CoV clone expressing the PREDICT/PDF-2180 spike protein was generated. Recombinant viruses derived from the clone were replication competent but unable to spread and establish new infections in Vero cells or primary human airway epithelial cells. Our findings suggest that PREDICT/PDF-2180 is unlikely to pose a zoonotic threat. Recombination in the S1 subunit of the spike gene was identified as the primary mechanism driving variation in the spike phenotype and was likely one of the critical steps in the evolution and emergence of MERS-CoV in humans.
has issue date	2017-04-04(xsd:dateTime)
bibo:doi	10.1128/mbio.00373-17
bibo:pmid	28377531
has license	cc-by
sha1sum (hex)	d90b2c4decfc17d7349325f437a9ab6082742c24
schema:url	https://doi.org/10.1128/mbio.00373-17
resource representing a document's title	Further Evidence for Bats as the Evolutionary Source of Middle East Respiratory Syndrome Coronavirus
has PubMed Central identifier	PMC5380844
has PubMed identifier	28377531
schema:publication	mBio
resource representing a document's body	covid:d90b2c4decfc17d7349325f437a9ab6082742c24#body_text
is schema:about of	named entity 'MERS-CoV' named entity 'strain' named entity 'receptor' named entity 'residues' named entity 'PDF' named entity 'Current' named entity 'Middle East respiratory syndrome' named entity 'driving' covid:arg/d90b2c4decfc17d7349325f437a9ab6082742c24 named entity 'VIRAL' named entity 'HUMANS' named entity 'PHENOTYPE' named entity 'CLONE' named entity 'JAK' named entity 'FAMILY VESPERTILIONIDAE' named entity 'RISK OF' named entity 'HTTPS' named entity 'PANDEMIC' named entity 'SPREAD' named entity 'UGANDA' named entity 'BEGINNING' named entity 'THESE' named entity 'ONE OF' named entity 'INVESTIGATED' named entity 'MAY HAVE' named entity 'SUGGEST' named entity 'PIPISTRELLUS' named entity 'SOURCE' named entity 'SUPPORTING' named entity 'RECOMBINATION' named entity 'RELATED' named entity 'PREVENTION' named entity 'EVIDENCE FOR' named entity 'DOES NOT' named entity 'RECEPTOR' named entity 'MERS-COV' named entity 'EVIDENCE' named entity 'PRIMARY' named entity 'HIGHLY' named entity 'LIKE' named entity 'STRATEGY' named entity 'VARIATION' named entity 'BINDING' named entity 'PREDICT' named entity 'IDENTIFIED' named entity 'SUBUNIT' named entity 'RECOMBINANT' named entity 'INSIGHTS' named entity 'REPLICATION' named entity 'VARIOUS'

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