About: The impact of viral transport media on PCR assay results for the detection of nucleic acid from SARS-CoV-2 and other viruses

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About: The impact of viral transport media on PCR assay results for the detection of nucleic acid from SARS-CoV-2 and other viruses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The impact of viral transport media on PCR assay results for the detection of nucleic acid from SARS-CoV-2 and other viruses
Creator	Kirkland, P Frost, M
Source	BioRxiv
abstract	During the 2020 SARS-Cov-2 pandemic, there has been an acute shortage of viral transport medium. Many different products have been used to meet the demands of large-scale diagnostic and surveillance testing. The stability of SARS-Cov-2 RNA was assessed in several commercially produced transport media and an in-house solution. Coronavirus RNA was rapidly destroyed in the commercial transport media though the deleterious effects on intact virus were limited. Similar results were obtained for a Type A influenza virus. There was reduced detection of both virus and nucleic acid when a herpesvirus sample and purified DNA were tested. Collectively these data showed that the commercial viral transport media contained nucleases or similar substances and may seriously compromise diagnostic and epidemiological investigations. Recommendations to include foetal bovine serum as a source of protein to enhance the stabilising properties of viral transport media are contraindicated. Almost all commercial batches of foetal bovine serum contain pestiviruses and at times other bovine viruses. In addition to the potential for there to be nucleases in the transport medium, the presence of these viruses and other extraneous nucleic acid in samples may compromise the interpretation of sequence data. The inclusion of foetal bovine serum presents a biosecurity risk for the movement of animal pathogens and renders these transport media unsuitable for animal disease diagnostic applications. While these transport media may be suitable for virus culture purposes, there could be misleading results if used for nucleic acid-based tests. Therefore, these products should be evaluated to ensure fitness for purpose.
has issue date	2020-06-10(xsd:dateTime)
bibo:doi	10.1101/2020.06.09.142323
has license	biorxiv
sha1sum (hex)	d900742851d847ecb31ac2db4d659d0071e8ba39
schema:url	https://doi.org/10.1101/2020.06.09.142323
resource representing a document's title	The impact of viral transport media on PCR assay results for the detection of nucleic acid from SARS-CoV-2 and other viruses
schema:publication	bioRxiv
resource representing a document's body	covid:d900742851d847ecb31ac2db4d659d0071e8ba39#body_text
is schema:about of	named entity 'SARS-Cov-2' named entity 'rapidly' named entity 'intact' named entity 'impact' named entity 'PANDEMIC' named entity 'OBTAINED' named entity 'VIRAL TRANSPORT' named entity 'SUBSTANCES' named entity 'EFFECTS' named entity 'TESTING' named entity 'TRANSPORT' named entity 'REDUCED' named entity 'SARS-COV-2 RNA' named entity 'DETECTION' named entity 'DIAGNOSTIC' named entity 'SOLUTION' named entity 'ACUTE' named entity 'RESULTS' covid:arg/d900742851d847ecb31ac2db4d659d0071e8ba39 named entity 'pandemic' named entity 'DNA' named entity 'commercial' named entity 'purified' named entity 'virus' named entity 'Type A' named entity 'commercially produced' named entity 'herpesvirus' named entity 'nucleic acid' named entity 'RNA' named entity 'serum' named entity 'VTM' named entity 'qPCR' named entity 'PBS' named entity 'virus' named entity 'Thermofisher Scientific' named entity 'viruses' named entity 'SARS-Cov-2' named entity 'viruses' named entity 'RNA' named entity 'nucleic acids' named entity 'homologous' named entity 'SARS-CoV-2' named entity 'nucleic acid' named entity 'SARS-CoV-2' named entity 'negative control' named entity 'VTM' named entity 'asymptomatic' named entity 'virus' named entity 'northern hemisphere' named entity 'experimental design' named entity 'VTM' named entity 'VTM' named entity 'P66' named entity 'viral RNA' named entity 'virus' named entity 'protein' named entity 'nucleic acid' named entity 'herpesvirus' named entity 'endogenous' named entity 'World Health Organisation' named entity 'Elizabeth Macarthur' named entity 'lysis buffer' named entity 'SARS-CoV-2' named entity 'RNA' named entity 'genome' named entity 'viruses' named entity 'VTM' named entity 'viruses' named entity 'VTM' named entity 'qRT-PCR' named entity 'VTM' named entity 'sequence data' named entity 'phosphate buffered saline' named entity 'SARS-CoV-2' named entity 'VTM' named entity 'RNA' named entity 'gelatin' named entity 'diluent' named entity 'BVDV' named entity 'Influenza A virus' named entity 'VTM' named entity 'nuclease' named entity 'phosphate buffered saline'

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