About: Coronaviridae and SARS-associated Coronavirus Strain HSR1

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About: Coronaviridae and SARS-associated Coronavirus Strain HSR1 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Coronaviridae and SARS-associated Coronavirus Strain HSR1
Creator	Canducci, Filippo Clementi, Massimo Bordignon, Claudio Carletti, Silvia Lazzarin, Adriano Mancini, Nicasio Pinna, Debora Poli, Guido Vicenzi, Elisa
Source	Medline; PMC
abstract	During the recent severe acute respiratory (SARS) outbreak, the etiologic agent was identified as a new coronavirus (CoV). We have isolated a SARS-associated CoV (SARS-CoV) strain by injecting Vero cells with a sputum specimen from an Italian patient affected by a severe pneumonia; the patient traveled from Vietnam to Italy in March 2003. Ultrastructural analysis of infected Vero cells showed the virions within cell vesicles and around the cell membrane. The full-length viral genome sequence was similar to those derived from the Hong-Kong Hotel M isolate. By using both real-time reverse transcription–polymerase chain reaction TaqMan assay and an infectivity plaque assay, we determined that approximately 360 viral genomes were required to generate a PFU. In addition, heparin (100 μg/mL) inhibited infection of Vero cells by 50%. Overall, the molecular and biologic characteristics of the strain HSR1 provide evidence that SARS-CoV forms a fourth genetic coronavirus group with distinct genomic and biologic features.
has issue date	2004-03-01(xsd:dateTime)
bibo:doi	10.3201/eid1003.030683
bibo:pmid	15109406
has license	no-cc
sha1sum (hex)	d8a0a04161c4ee9b42ffddb705b21ab539098447
schema:url	https://doi.org/10.3201/eid1003.030683
resource representing a document's title	Coronaviridae and SARS-associated Coronavirus Strain HSR1
has PubMed Central identifier	PMC3322807
has PubMed identifier	15109406
schema:publication	Emerg Infect Dis
resource representing a document's body	covid:d8a0a04161c4ee9b42ffddb705b21ab539098447#body_text
is schema:about of	named entity 'infection' named entity 'viral genomes' named entity 'LENGTH' named entity 'ITALIAN' named entity 'GENERATE' named entity 'PLAQUE ASSAY' named entity 'ETIOLOGIC AGENT' named entity 'SEVERE PNEUMONIA' named entity 'CELL' named entity 'ASSAY' named entity 'HAVE' named entity 'OUTBREAK' named entity 'HEPARIN' named entity 'GENETIC' named entity 'FORMS' named entity 'ADDITION' named entity 'MOLECULAR' named entity 'INFECTIVITY' named entity 'FOURTH' named entity 'HONG' named entity 'INJECTING' named entity 'CHARACTERISTICS' named entity 'CORONAVIRUS GROUP' named entity 'DERIVED' named entity 'IDENTIFIED' named entity 'MG%' named entity '28100' named entity 'VESICLES' named entity 'SARS-COV' named entity 'GENOMIC' named entity 'SPUTUM SPECIMEN' named entity '50%' named entity 'CELL MEMBRANE' named entity 'FEATURES' named entity 'INFECTED' named entity 'PATIENT AFFECTED BY' named entity 'PFU' named entity 'ITALY' named entity 'CORONAVIRIDAE' named entity 'ASSOCIATED' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'TAQMAN' named entity 'GENOMES' named entity 'DETERMINED' named entity 'PATIENT' named entity 'RECENT' named entity 'VIRIONS' named entity 'DISTINCT' named entity 'ISOLATE' named entity 'VIRAL' named entity 'VERO CELLS' named entity 'VIETNAM' named entity 'HSR1' named entity 'REQUIRED' named entity 'HOTEL' named entity 'GENOME SEQUENCE' named entity 'ISOLATED' named entity 'CORONAVIRUS' named entity 'POLYMERASE CHAIN REACTION' named entity 'APPROXIMATELY' named entity 'INHIBITED' named entity 'NEW' named entity 'EVIDENCE' named entity 'REVERSE TRANSCRIPTION' named entity 'VIRAL GENOME' named entity '360' named entity 'BIOLOGIC' named entity 'OVERALL' named entity 'STRAIN' named entity 'HSR1' named entity 'PROVIDE' named entity 'USING' named entity 'MARCH'

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