About: Enhancement of target-DNA hybridization efficiency by pre-hybridization on sequence-orientated micro-arrayed probes

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About: Enhancement of target-DNA hybridization efficiency by pre-hybridization on sequence-orientated micro-arrayed probes Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Enhancement of target-DNA hybridization efficiency by pre-hybridization on sequence-orientated micro-arrayed probes
Creator	Chen, Chia-Chun Huang, Jie-Len Su, Hung-Ju Wu, Jui-Chuang Yang, Dan-Kai
Source	Elsevier; PMC
abstract	Abstract The enhancement of hybridization efficiency of deoxyribonucleic acid (DNA) targets using oligonucleotide pre-hybridization is studied on two sequence-inversed micro-arrayed probes. The sequences for pre-hybridizing both oligo and target DNA are designed to be fully complementary with their shared DNA probe in a coaxial stacking configuration; i.e. they hybridize immediately alongside each other along the continuous complement probe strand. The pre-hybridizing oligo and target DNA are differentiated by being labeled with two distinct fluorescent dyes, and the cooperative effect on hybridization efficiency is investigated through the comparison of the stacking and individual hybridization configurations based on the detection signals of the labeling dyes. The results show that the pre-hybridization of a DNA oligo enhances the subsequent hybridization efficiency of the target-DNA coupling onto the same probe. The efficiency is enhanced if the hybridization position occurs at a site close to the substrate surface.
has issue date	2008-05-31(xsd:dateTime)
bibo:doi	10.1016/j.jcice.2007.12.012
has license	els-covid
sha1sum (hex)	d5fe64c1a40f8bd7aa905ec2ae30e911eacadb53
schema:url	https://doi.org/10.1016/j.jcice.2007.12.012
resource representing a document's title	Enhancement of target-DNA hybridization efficiency by pre-hybridization on sequence-orientated micro-arrayed probes
has PubMed Central identifier	PMC7148900
schema:publication	Journal of the Chinese Institute of Chemical Engineers
resource representing a document's body	covid:d5fe64c1a40f8bd7aa905ec2ae30e911eacadb53#body_text
is schema:about of	named entity 'SEQUENCE' named entity 'labeled' named entity 'targets' named entity 'configuration' named entity 'DNA HYBRIDIZATION' named entity 'SURFACE' named entity 'POSITION' named entity 'DNA' named entity 'DNA OLIGO' named entity 'COMPLEMENT' named entity 'BASED' named entity 'MICRO-' named entity 'INDIVIDUAL' named entity 'SITE' named entity 'SEQUENCE' named entity 'SUBSEQUENT' named entity 'DISTINCT' named entity 'FLUORESCENT DYES' named entity 'DYES' named entity 'COOPERATIVE' named entity 'LABELING' named entity 'OLIGO' named entity 'ENHANCED' named entity 'TARGETS' named entity 'EFFICIENCY' named entity 'PRE' named entity 'OLIGONUCLEOTIDE' named entity 'CONFIGURATION' named entity 'DNA PROBE' named entity 'FULLY' named entity 'PROBES' named entity 'MICRO-' named entity 'DEOXYRIBONUCLEIC ACID' named entity 'EFFICIENCY' named entity 'ENHANCEMENT' named entity 'TARGET' named entity 'SIGNALS' named entity 'LABELED' named entity 'THEIR' named entity 'PROBE' named entity 'SEQUENCES' named entity 'USING' named entity 'HYBRIDIZATION' named entity 'TARGET' named entity 'SUBSTRATE' named entity 'CONTINUOUS' named entity 'STACKING' named entity 'EFFECT' named entity 'INVESTIGATED' named entity 'COMPARISON' named entity 'BEING' named entity 'IMMEDIATELY' named entity 'COUPLING' named entity 'DETECTION' named entity 'ENHANCEMENT' named entity 'DIFFERENTIATED' named entity 'HYBRIDIZATION' named entity 'CLOSE TO' named entity 'SHARED' named entity 'RESULTS' named entity 'PRE' named entity 'PROBES' named entity 'ORIENTATED' named entity 'STRAND' named entity 'A DNA' covid:arg/d5fe64c1a40f8bd7aa905ec2ae30e911eacadb53 named entity 'enhancement' named entity 'site' named entity 'hybridization' named entity 'fluorescent dyes' named entity 'probes' named entity 'probe' named entity 'studied' named entity 'coaxial stacking' named entity 'individual' named entity 'DNA' named entity 'hybridization' named entity 'hybridization' named entity 'DNA'

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