About: A Murine and a Porcine Coronavirus Are Released from Opposite Surfaces of the Same Epithelial Cells

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: A Murine and a Porcine Coronavirus Are Released from Opposite Surfaces of the Same Epithelial Cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A Murine and a Porcine Coronavirus Are Released from Opposite Surfaces of the Same Epithelial Cells
Creator	Rottier, P Dveksler, G Horzinek, M Rossen, J Strous, G Bekker, C Holmes, K
Source	Elsevier; Medline; PMC
abstract	Abstract Epithelial cells are important target cells for coronavirus infection. Earlier we have shown that transmissible gastroenteritis coronavirus (TGEV) and mouse hepatitis coronavirus (MHV) are released from different sides of porcine and murine epithelial cells, respectively. To study the release of these viruses from the same cells, we constructed a porcine LLC-PK1 cell line stably expressing the recombinant MHV receptor cDNA (LMR cells). The MHV and TGEV receptor glycoproteins were shown by immunofluorescence to appear at the surface of the cells and to be functional so that the cells were susceptible to both MHV and TGEV infection. Both coronaviruses entered polarized LMR cells only through the apical surface. Remarkably, while the cells remained susceptible to TGEV for long periods, infectability by MHV decreased with time after plating of the cells onto filters. This was not due to a lack of expression of the MHV receptor, since this glycoprotein was still abundant on the apical surface of these cells. TGEV and MHV appeared to exit LMR cells from opposite sides. Whereas TGEV was released preferentially at the apical membrane, MHV was released preferentially at the basolateral surface. These results show that vesicles containing the two coronaviruses are targeted differently in LMR cells. We propose that the viruses are sorted at the Golgi complex into different transport vesicles that carry information directing them to one of the two surface domains. The apical release of TGEV and the basolateral release of MHV might be factors contributing to the difference in virus spread found between TGEV and MHV in their respective natural hosts, the former causing mainly a localized enteric infection, the latter spreading through the body to other organs.
has issue date	1996-10-01(xsd:dateTime)
bibo:doi	10.1006/viro.1996.0540
bibo:pmid	8862433
has license	els-covid
sha1sum (hex)	d5c88aca906ccfc64bc6f7515299185a65d44ab6
schema:url	https://doi.org/10.1006/viro.1996.0540
resource representing a document's title	A Murine and a Porcine Coronavirus Are Released from Opposite Surfaces of the Same Epithelial Cells
has PubMed Central identifier	PMC7130667
has PubMed identifier	8862433
schema:publication	Virology
resource representing a document's body	covid:d5c88aca906ccfc64bc6f7515299185a65d44ab6#body_text
is schema:about of	named entity 'CORONAVIRUS' named entity 'Opposite' named entity 'SURFACES' named entity 'EPITHELIAL CELLS' named entity 'COMMUNICATION' named entity 'RELEASED' named entity 'PORCINE' named entity 'MURINE' named entity 'OPPOSITE' named entity 'SHORT' named entity 'Epithelial Cells' named entity 'Porcine' named entity 'plasma membrane' named entity 'glycoprotein' named entity 'receptor' named entity 'inoculation' named entity 'protein synthesis' named entity 'glycoproteins' named entity 'MHV' named entity 'apical membrane' named entity 'MHV' named entity 'glycoprotein' named entity 'EGTA' named entity 'Sindbis' named entity 'monoclonal antibody' named entity 'apical membrane' named entity 'spike proteins' named entity 'infection' named entity 'MHV' named entity 'immunofluorescence' named entity 'coronavirus' named entity 'cDNA' named entity 'infectivity' named entity 'epithelial cells' named entity 'MHV' named entity 'epithelial cells' named entity 'viruses' named entity 'viruses' named entity 'MHV' named entity 'metabolically active' named entity 'coronaviruses' named entity 'MHV' named entity 'immunoisolation' named entity 'Geneticin' named entity 'serum' named entity 'virus' named entity 'receptor protein' named entity 'measles virus' named entity 'vesicles' named entity 'viruses' named entity 'viral particles' named entity 'virus' named entity 'infection' named entity 'coronavirus' named entity 'viruses' named entity 'glycoprotein' named entity 'Golgi complex' named entity 'infection' named entity 'viruses' named entity 'virions' named entity 'spike protein' named entity 'immunofluorescence' named entity 'virus' named entity 'porcine' named entity 'subclinical' named entity 'transmissible gastroenteritis coronavirus' named entity 'membrane proteins' named entity 'exocytosis' named entity 'viral particles' named entity 'plasma membrane' named entity 'N-glycosylated' named entity 'plasmid' named entity 'glycoprotein' named entity 'murine' named entity 'immunofluorescence'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software