About: A novel quantitative PCR mediated by high-fidelity DNA polymerase

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About: A novel quantitative PCR mediated by high-fidelity DNA polymerase Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A novel quantitative PCR mediated by high-fidelity DNA polymerase
Creator	Zhang, Chiyu Jin, Xia Li, Yang Zhu, Xiaoli Lin, Yi Hu, Yihong Liu, Kyle Zhang, Mengling Zhong, Ping
Source	PMC
abstract	The biggest challenge for accurate diagnosis of viral infectious disease is the high genetic variability of involved viruses, which affects amplification efficiency and results in low sensitivity and narrow spectrum. Here, we developed a new simple qPCR mediated by high-fidelity (HF) DNA polymerase. The new method utilizes an HFman probe and one primer. Fluorescent signal was generated from the 3′–5′ hydrolysis of HFman probe by HF DNA polymerase before elongation initiation. Mismatches between probe/primer and template have less influence on the amplification efficiency of the new method. The new qPCR exhibited higher sensitivity and better adaptability to sequence variable templates than the conventional TaqMan probe based-qPCR in quantification of HIV-1 viral load. Further comparison with COBAS TaqMan HIV-1 Test (v2.0) showed a good correlation coefficient (R(2) = 0.79) between both methods in quantification of HIV-1 viral load among 21 clinical samples. The characteristics of tolerance to variable templates and one probe-one primer system imply that the probe/primer design for the new method will be easier and more flexible than the conventional method for highly heterogeneous viruses. Therefore, the HF DNA polymerase-mediated qPCR method is a simple, sensitive and promising approach for the development of diagnostics for viral infectious diseases.
has issue date	2017-09-04(xsd:dateTime)
bibo:doi	10.1038/s41598-017-10782-4
bibo:pmid	28871131
has license	cc-by
sha1sum (hex)	d55ee40e7898b693872356e1661f658732984bae
schema:url	https://doi.org/10.1038/s41598-017-10782-4
resource representing a document's title	A novel quantitative PCR mediated by high-fidelity DNA polymerase
has PubMed Central identifier	PMC5583327
has PubMed identifier	28871131
schema:publication	Sci Rep
resource representing a document's body	covid:d55ee40e7898b693872356e1661f658732984bae#body_text
is schema:about of	named entity 'Therefore' named entity 'qPCR' named entity 'primer' named entity 'The' named entity 'developed' named entity 'signal' named entity 'mediated' named entity 'NOVEL' named entity 'HAVE' named entity 'TAQMAN' named entity 'SENSITIVE' named entity 'DIAGNOSTICS' named entity 'HIV-1' named entity 'GENERATED' named entity 'VIRAL LOAD' named entity 'NEW' named entity 'SEQUENCE' named entity 'GOOD' named entity 'DIAGNOSIS' named entity 'SYSTEM' named entity 'DNA POLYMERASE' named entity 'CLINICAL' named entity 'CHARACTERISTICS' named entity 'COMPARISON' named entity 'HIGH' named entity 'BASED' named entity 'PROBE' named entity 'QPCR' named entity 'MEDIATED' named entity 'NARROW SPECTRUM' named entity 'AMPLIFICATION' named entity 'HERE' named entity 'VIRAL INFECTIOUS DISEASES' named entity 'SAMPLES' named entity 'ACCURATE' named entity 'METHOD' named entity 'PRIMER' named entity 'FIDELITY' named entity 'FLUORESCENT' named entity 'QUANTIFICATION' named entity 'SIMPLE' named entity 'HIGH' named entity 'INFLUENCE' named entity 'FIDELITY' named entity 'MEDIATED' named entity 'DNA POLYMERASE' named entity 'APPROACH' named entity 'VIRUSES' named entity 'TEST' named entity 'CORRELATION COEFFICIENT' named entity 'ELONGATION' named entity 'ADAPTABILITY' named entity 'VIRAL INFECTIOUS DISEASE' named entity 'VARIABLE' named entity 'RESULTS' named entity 'LOW' named entity 'TOLERANCE' named entity 'DEVELOPMENT' named entity 'OPEN' named entity 'QUANTITATIVE PCR' named entity 'METHODS' named entity 'CONVENTIONAL' named entity 'TEMPLATE' named entity 'EFFICIENCY' named entity 'IS A' named entity 'HETEROGENEOUS' named entity 'HYDROLYSIS' named entity 'BETTER' named entity 'INVOLVED' named entity 'GENETIC VARIABILITY' named entity 'CHALLENGE' named entity 'FLEXIBLE' named entity 'SENSITIVITY'

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