About: A general strategy to inhibiting viral −1 frameshifting based on upstream attenuation duplex formation

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About: A general strategy to inhibiting viral −1 frameshifting based on upstream attenuation duplex formation Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A general strategy to inhibiting viral −1 frameshifting based on upstream attenuation duplex formation
Creator	Chang, Kung-Yao Cho, Che-Pei Lin, Ya-Hui Hu, Hao-Teng
source	Medline; PMC
abstract	Viral −1 programmed ribosomal frameshifting (PRF) as a potential antiviral target has attracted interest because many human viral pathogens, including human immunodeficiency virus (HIV) and coronaviruses, rely on −1 PRF for optimal propagation. Efficient eukaryotic −1 PRF requires an optimally placed stimulator structure downstream of the frameshifting site and different strategies targeting viral −1 PRF stimulators have been developed. However, accessing particular −1 PRF stimulator information represents a bottle-neck in combating the emerging epidemic viral pathogens such as Middle East respiratory syndrome coronavirus (MERS-CoV). Recently, an RNA hairpin upstream of frameshifting site was shown to act as a cis-element to attenuate −1 PRF with mechanism unknown. Here, we show that an upstream duplex formed in-trans, by annealing an antisense to its complementary mRNA sequence upstream of frameshifting site, can replace an upstream hairpin to attenuate −1 PRF efficiently. This finding indicates that the formation of a proximal upstream duplex is the main determining factor responsible for −1 PRF attenuation and provides mechanistic insight. Additionally, the antisense-mediated upstream duplex approach downregulates −1 PRF stimulated by distinct −1 PRF stimulators, including those of MERS-CoV, suggesting its general application potential as a robust means to evaluating viral −1 PRF inhibition as soon as the sequence information of an emerging human coronavirus is available.
has issue date	2016-01-08(xsd:dateTime)
bibo:doi	10.1093/nar/gkv1307
bibo:pmid	26612863
has license	cc-by
sha1sum (hex)	d4d885e748fc8335d359da08f2ef0370103f98c7
schema:url	https://doi.org/10.1093/nar/gkv1307
resource representing a document's title	A general strategy to inhibiting viral −1 frameshifting based on upstream attenuation duplex formation
has PubMed Central identifier	PMC4705660
has PubMed identifier	26612863
schema:publication	Nucleic Acids Res
resource representing a document's body	covid:d4d885e748fc8335d359da08f2ef0370103f98c7#body_text
is schema:about of	named entity 'ATTENUATION' named entity 'PRF' named entity 'PRF' named entity 'mRNA' named entity 'developed' named entity 'inhibition' named entity 'coronaviruses' named entity 'structure' named entity 'site' named entity 'insight' named entity 'PRF' named entity 'strategy' named entity 'FORMATION' named entity 'CORONAVIRUSES' named entity 'EPIDEMIC' named entity 'MEANS' named entity 'TARGETING' named entity 'STRUCTURE' named entity 'INCLUDING' named entity 'UPSTREAM' named entity 'ATTENUATE' named entity 'PRF' named entity 'GENERAL' named entity 'ACCESSING' named entity 'PROXIMAL' named entity 'PROPAGATION' named entity 'RIBOSOMAL' named entity 'SEQUENCE' named entity 'PLACED' named entity 'DUPLEX' named entity 'ATTENUATION' named entity 'ITS' named entity 'APPLICATION' named entity 'FORMATION' named entity 'PARTICULAR' named entity 'CIS' named entity 'INHIBITION' named entity 'TRANS' named entity 'ACT' named entity 'VIRAL' named entity 'DOWNSTREAM' named entity 'HAVE' named entity 'FINDING' named entity 'VIRAL PATHOGENS' named entity 'EFFICIENT' named entity 'REPLACE' named entity 'HUMAN IMMUNODEFICIENCY VIRUS' named entity 'INFORMATION' named entity 'POTENTIAL' named entity 'EUKARYOTIC' named entity 'STIMULATOR' named entity 'ROBUST' named entity 'UPSTREAM' named entity 'VIRAL' named entity 'DISTINCT' named entity 'PROVIDES' named entity 'ANTIVIRAL' named entity 'FORMED' named entity 'AVAILABLE' named entity 'BASED' named entity 'INHIBITING' named entity 'STRATEGY' named entity 'GENERAL' named entity 'SOON' named entity 'STIMULATED' named entity 'HUMAN CORONAVIRUS' named entity 'MEDIATED' named entity 'HUMAN' named entity 'SITE' named entity 'HERE' named entity 'MRNA' named entity 'MECHANISM' named entity 'FACTOR' named entity 'ANTISENSE' named entity 'UNKNOWN' named entity 'INTEREST' named entity 'MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS' named entity 'ANNEALING'

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