About: Development of transgenic mice expressing a coronavirus-specific public CD4 T cell receptor

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of transgenic mice expressing a coronavirus-specific public CD4 T cell receptor
Creator	Zhao, Jincun Perlman, Stanley Zhao, Jingxian Fett, Craig Pewe, Lecia
Source	Elsevier; Medline; PMC
abstract	Mice that are transgenic (Tg) for T cell receptor (TCR) expression are used extensively to analyze longitudinal T cell responses during effector and memory phases of the T cell response. Generation of TCR Tg mice generally requires T cell stimulation and cloning in vitro prior to amplification, processes which introduce biases into selection of the TCR that is ultimately chosen for TCR Tg mouse generation. Here we describe an alternative approach that involves no T cell stimulation or propagation in vitro. We generated mice that were transgenic for a TCR responding to a CD4 T cell epitope (epitope M133) that is immunodominant in mice infected with a neurotropic coronavirus, the JHM strain of mouse hepatitis virus. The CD4 T cell response to epitope M133 is of particular interest because it may be pathogenic, protective or regulatory, depending upon the physiological setting. We applied an iterative process in which we identified a TCR-β chain expressed by all mice that were examined (‘public sequence’). This TCR-β chain was introduced into bone marrow cells with a lentivirus vector, generating TCR-β retrogenic mice. A TCR-α chain that paired with this TCR-β was then identified and used to generate a second set of TCR (α/β) retrogenic mice. After demonstrating that these cells were functional and responded to epitope M133, these TCR chains were used to generate an epitope M133-specific TCR Tg mouse. This method should be generally useful for engineering TCR Tg mice without introduction of bias caused by in vitro manipulation and propagation.
has issue date	2013-10-01(xsd:dateTime)
bibo:doi	10.1016/j.jim.2013.07.011
bibo:pmid	23928495
has license	bronze-oa
sha1sum (hex)	d488d97d9ce77bbe110b5723ec3c83119793a4ab
schema:url	https://doi.org/10.1016/j.jim.2013.07.011
resource representing a document's title	Development of transgenic mice expressing a coronavirus-specific public CD4 T cell receptor
has PubMed Central identifier	PMC3850057
has PubMed identifier	23928495
schema:publication	Journal of Immunological Methods
resource representing a document's body	covid:d488d97d9ce77bbe110b5723ec3c83119793a4ab#body_text
is schema:about of	named entity 'PUBLIC' named entity 'transgenic' named entity 'selection' named entity 'functional' named entity 'effector' named entity 'T cell' named entity 'epitope' named entity 'transgenic mice' named entity 'INTRODUCTION' named entity 'SELECTION' named entity 'NEUROTROPIC' named entity 'DESCRIBE' named entity 'EFFECTOR' named entity 'SET' named entity 'CD4' named entity 'GENERATING' named entity 'STRAIN' named entity 'PAIRED' covid:arg/d488d97d9ce77bbe110b5723ec3c83119793a4ab named entity 'TRANSGENIC MICE' named entity 'AMPLIFICATION' named entity 'BIASES' named entity 'FUNCTIONAL' named entity 'T CELL' named entity 'CHAIN' named entity 'TCR' named entity 'PROPAGATION' named entity 'ENGINEERING' named entity 'THESE' named entity 'EXPRESSED' named entity 'INTEREST' named entity 'BIAS' named entity 'MOUSE' named entity 'PARTICULAR' named entity 'SPECIFIC' named entity 'DEVELOPMENT' named entity 'MICE' named entity 'CLONING' named entity 'EPITOPE' named entity 'ALTERNATIVE' named entity 'PATHOGENIC' named entity 'T CELL RESPONSE' named entity 'PRIOR TO' named entity 'CORONAVIRUS' named entity 'CD4' named entity 'CORONAVIRUS' named entity 'MEMORY' named entity 'USED' named entity 'SECOND' named entity 'T CELL EPITOPE' named entity 'TRANSGENIC' named entity 'LONGITUDINAL' named entity 'SPECIFIC' named entity 'T CELL RECEPTOR' named entity 'LENTIVIRUS VECTOR' named entity 'APPLIED' named entity 'BONE MARROW CELLS' named entity 'PROCESS' named entity 'PROCESSES' named entity 'IN VITRO' named entity 'INTRODUCE' named entity 'IDENTIFIED' named entity 'MANIPULATION' named entity 'RESPONSES' named entity 'PHYSIOLOGICAL' named entity 'STIMULATION' named entity 'CAUSED BY' named entity 'METHOD' named entity 'GENERALLY' named entity 'EXPRESSION' named entity 'PHASES' named entity 'HERE' named entity 'MAY BE' named entity 'RESPONSE TO' named entity 'APPROACH' named entity 'MOUSE HEPATITIS VIRUS' named entity 'INTRODUCED INTO'

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