About: PCR assay for the detection and the identification of atypical canine coronavirus in dogs

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About: PCR assay for the detection and the identification of atypical canine coronavirus in dogs Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	PCR assay for the detection and the identification of atypical canine coronavirus in dogs
Creator	Buonavoglia, Canio Decaro, Nicola Elia, Gabriella Tinelli, Antonella Pratelli, Annamaria Camero, Michele Gentile, Arturo
Source	Elsevier; Medline; PMC
abstract	Comparative sequence analysis of the PCR products of the M gene and fragments of the pol1a and pol1b genes of canine coronavirus (CCoV) have demonstrated that two separate clusters of CCoV are present in dogs. This note describes a PCR assay to identify atypical CCoV strains with nucleotide substitutions in the M gene. A total of 177 faecal samples from dogs CCoV positive previously with the PCR assay were analysed. Sixty-two of the 177 samples were amplified with the PCR described in the present study and were thus considered atypical CCoVs. The specificity of the PCR typing assay was confirmed by sequence analysis of the PCR products.
has issue date	2002-10-01(xsd:dateTime)
bibo:doi	10.1016/s0166-0934(02)00165-9
bibo:pmid	12393151
has license	no-cc
sha1sum (hex)	d48670c17f5f686dcff628d703cb0b7c13e22aac
schema:url	https://doi.org/10.1016/s0166-0934%2802%2900165-9
resource representing a document's title	PCR assay for the detection and the identification of atypical canine coronavirus in dogs
has PubMed Central identifier	PMC7119860
has PubMed identifier	12393151
schema:publication	J Virol Methods
resource representing a document's body	covid:d48670c17f5f686dcff628d703cb0b7c13e22aac#body_text
is schema:about of	named entity 'CANINE CORONAVIRUS' named entity 'PCR' named entity 'assay' named entity 'PCR' named entity 'gene' named entity 'samples' named entity 'assay' named entity 'canine coronavirus' named entity 'DETECTION' named entity 'PCR' named entity 'ATYPICAL' named entity 'TYPING' named entity 'PRESENT' named entity 'CANINE CORONAVIRUS' named entity 'PREVIOUSLY' named entity 'ASSAY' named entity 'SAMPLES' named entity 'STRAINS' named entity 'SEPARATE' named entity 'DOGS' named entity 'PCR ASSAY' named entity 'ATYPICAL' named entity 'SPECIFICITY' named entity 'GENES' named entity 'SIXTY' named entity 'POSITIVE' named entity 'PRODUCTS' named entity 'STUDY' named entity 'SEQUENCE ANALYSIS' named entity 'NOTE' named entity 'IDENTIFICATION' named entity 'FRAGMENTS' named entity 'PCR ASSAY' named entity 'TO IDENTIFY' named entity 'NUCLEOTIDE' named entity 'CONSIDERED' named entity 'M GENE' named entity 'COMPARATIVE SEQUENCE ANALYSIS' named entity 'TOTAL' named entity 'FAECAL' named entity 'DESCRIBED' named entity 'DEMONSTRATED' named entity 'AMPLIFIED' named entity 'CLUSTERS' named entity 'HAVE' named entity 'CONFIRMED BY' named entity 'DOGS' named entity 'Comparative sequence analysis' named entity 'faecal samples' named entity 'assay' named entity 'FIPV' named entity 'gene' named entity 'FCoV' named entity 'PCR' named entity 'variable region' named entity 'PCR' named entity 'protein' named entity 'FCoV' named entity 'c-DNA' named entity 'feline infectious peritonitis virus' named entity 'FCoV' named entity 'electron' named entity 'denaturing' named entity 'FCoV' named entity 'sequence analysis' named entity 'reverse transcription' named entity 'epizootic' named entity 'faeces' named entity 'virus' named entity 'vomiting' named entity 'nucleotide' named entity 'swine' named entity 'faecal samples' named entity 'PCR' named entity 'FIPV'

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