About: Resistance to coronavirus infection in amino peptidase N-deficient pigs

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Resistance to coronavirus infection in amino peptidase N-deficient pigs
Creator	Ada, G Alan, Mileham Cino-Ozuna, Ying Fang, Kevin, D Maureen, Sheahan Melissa, S Prather, Randall, S Raymond, R Richard, Hesse Vlad, Petrovan Wells, Whitworth, Kristin
Source	Medline; PMC
abstract	The alphacoronaviruses, transmissible gastroenteritis virus (TGEV) and Porcine epidemic diarrhea virus (PEDV) are sources of high morbidity and mortality in neonatal pigs, a consequence of dehydration caused by the infection and necrosis of enterocytes. The biological relevance of amino peptidase N (ANPEP) as a putative receptor for TGEV and PEDV in pigs was evaluated by using CRISPR/Cas9 to edit exon 2 of ANPEP resulting in a premature stop codon. Knockout pigs possessing the null ANPEP phenotype and age matched wild type pigs were challenged with either PEDV or TGEV. Fecal swabs were collected daily from each animal beginning 1 day prior to challenge with PEDV until the termination of the study. The presence of virus nucleic acid was determined by PCR. ANPEP null pigs did not support infection with TGEV, but retained susceptibility to infection with PEDV. Immunohistochemistry confirmed the presence of PEDV reactivity and absence of TGEV reactivity in the enterocytes lining the ileum in ANPEP null pigs. The different receptor requirements for TGEV and PEDV have important implications in the development of new genetic tools for the control of enteric disease in pigs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11248-018-0100-3) contains supplementary material, which is available to authorized users.
has issue date	2018-10-12(xsd:dateTime)
bibo:doi	10.1007/s11248-018-0100-3
bibo:pmid	30315482
has license	cc-by
sha1sum (hex)	d4766bca29ba8c705eed4b4a721476ccf300fa21
schema:url	https://doi.org/10.1007/s11248-018-0100-3
resource representing a document's title	Resistance to coronavirus infection in amino peptidase N-deficient pigs
has PubMed Central identifier	PMC6353812
has PubMed identifier	30315482
schema:publication	Transgenic Res
resource representing a document's body	covid:d4766bca29ba8c705eed4b4a721476ccf300fa21#body_text
is schema:about of	named entity 'EVALUATED' named entity 'RELEVANCE' named entity 'RESULTING IN' named entity 'LINING' named entity 'PREMATURE STOP CODON' named entity 'HAVE' named entity 'DEHYDRATION' named entity 'COLLECTED' named entity 'CAS9' named entity 'DETERMINED BY' named entity 'REQUIREMENTS' named entity 'TERMINATION' named entity 'PRESENCE OF VIRUS' named entity 'BUT' named entity '1 DAY' named entity 'PCR' named entity 'CONTROL' named entity 'RECEPTOR' named entity 'NULL' named entity 'EDIT' named entity 'ANIMAL' named entity 'CONSEQUENCE OF' named entity 'PIGS' named entity 'TOOLS' named entity 'AMINO' named entity 'infection' named entity 'neonatal' named entity 'caused' named entity 'dehydration' named entity 'infection' covid:arg/d4766bca29ba8c705eed4b4a721476ccf300fa21 named entity 'SUSCEPTIBILITY TO INFECTION' named entity 'NECROSIS' named entity 'ENTEROCYTES' named entity 'WILD TYPE' named entity 'CONFIRMED' named entity 'BIOLOGICAL' named entity 'INFECTION' named entity 'CHALLENGE' named entity 'PEPTIDASE N' named entity 'DISEASE' named entity 'IMMUNOHISTOCHEMISTRY' named entity 'ABSENCE OF' named entity 'AMINO' named entity 'SWABS' named entity 'PRESENCE OF' named entity 'SUPPORT' named entity 'CRISPR' named entity 'DIFFERENT' named entity 'RETAINED' named entity 'PIGS' named entity 'PEPTIDASE N' named entity 'CORONAVIRUS INFECTION' named entity 'DEFICIENT' named entity 'BEGINNING' named entity 'PHENOTYPE' named entity 'NEONATAL' named entity 'EXON' named entity 'EITHER' named entity 'DEVELOPMENT' named entity 'MATCHED' named entity 'GENETIC' named entity 'NEW' named entity 'USING' named entity 'ENTERIC' named entity 'ILEUM' named entity 'TRANSMISSIBLE GASTROENTERITIS VIRUS' named entity 'SOURCES'

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