About: Efficient hepatic delivery and protein expression enabled by optimized mRNA and ionizable lipid nanoparticle

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Efficient hepatic delivery and protein expression enabled by optimized mRNA and ionizable lipid nanoparticle Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Efficient hepatic delivery and protein expression enabled by optimized mRNA and ionizable lipid nanoparticle
Creator	Liang, Zicai Li, Chunhui Wang, Xiaoxia Cao, Huiqing Huang, Yuanyu Liang, Xing-Jie Weng, Yuhua Xiao, Haihua Yang, Tongren Zhang, Mengjie Zhao, Deyao
Source	Elsevier; Medline; PMC
abstract	mRNA is a novel class of therapeutic modality that holds great promise in vaccination, protein replacement therapy, cancer immunotherapy, immune cell engineering etc. However, optimization of mRNA molecules and efficient in vivo delivery are quite important but challenging for its broad application. Here we present an ionizable lipid nanoparticle (iLNP) based on iBL0713 lipid for in vitro and in vivo expression of desired proteins using codon-optimized mRNAs. mRNAs encoding luciferase or erythropoietin (EPO) were prepared by in vitro transcription and formulated with proposed iLNP, to form iLP171/mRNA formulations. It was revealed that both luciferase and EPO proteins were successfully expressed by human hepatocellular carcinoma cells and hepatocytes. The maximum amount of protein expression was found at 6 h post-administration. The expression efficiency of EPO with codon-optimized mRNA was significantly higher than that of unoptimized mRNA. Moreover, no toxicity or immunogenicity was observed for these mRNA formulations. Therefore, our study provides a useful and promising platform for mRNA therapeutic development.
has issue date	2020-07-13(xsd:dateTime)
bibo:doi	10.1016/j.bioactmat.2020.07.003
bibo:pmid	32691013
has license	no-cc
sha1sum (hex)	d3549ea95e4dc1fd6853ae0d2c944953c1599e78
schema:url	https://doi.org/10.1016/j.bioactmat.2020.07.003
resource representing a document's title	Efficient hepatic delivery and protein expression enabled by optimized mRNA and ionizable lipid nanoparticle
has PubMed Central identifier	PMC7355334
has PubMed identifier	32691013
schema:publication	Bioact Mater
resource representing a document's body	covid:d3549ea95e4dc1fd6853ae0d2c944953c1599e78#body_text
is schema:about of	named entity 'therapy' named entity 'luciferase' named entity 'optimization' named entity 'mRNA' named entity 'Lipid' named entity 'great' named entity 'protein' named entity 'ITS' named entity 'THESE' named entity 'PROPOSED' named entity 'EXPRESSION' named entity 'BASED' named entity 'HEPATOCELLULAR CARCINOMA' named entity 'EPO' named entity 'PROTEIN REPLACEMENT THERAPY' named entity 'mRNA' named entity 'erythropoietin' named entity 'prepared' named entity 'mRNA' named entity 'application' named entity 'human' named entity 'encoding' named entity 'EPO' named entity 'promise' named entity 'transcription' named entity 'expression' named entity 'mRNA' named entity 'Therefore' named entity 'protein expression' named entity 'codon' named entity 'EPO' named entity 'mRNA therapeutic' named entity 'mRNA' named entity 'lipid' named entity 'iLNP' named entity 'EPO' named entity 'EPO' named entity 'vaccination' named entity 'ionizable' named entity 'lipid' named entity 'mRNA' named entity 'nanoparticle' named entity 'intravenously injected' named entity 'qRT-PCR' named entity 'sulfonic acid' named entity 'serum' named entity 'mice' named entity 'mice' named entity 'siRNA' named entity 'cLNP' named entity 'mRNA' named entity 'therapeutic protein' named entity 'nucleic acid' named entity 'lung' named entity 'siRNA' named entity 'vaccination' named entity 'liver' named entity 'lipid' named entity 'transfection' named entity 'FACS' named entity 'siRNA' named entity 'BioTek' named entity 'protein expression' named entity 'St Louis' named entity 'mRNA' named entity 'Suzhou' named entity 'mRNA vaccine' named entity 'fluorescence-activated cell sorting' named entity 'fluorescence' named entity 'mpk' named entity 'pKa'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software