About: Expression and biochemical characterization of nsP2 cysteine protease of Chikungunya virus

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About: Expression and biochemical characterization of nsP2 cysteine protease of Chikungunya virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Expression and biochemical characterization of nsP2 cysteine protease of Chikungunya virus
Creator	Pastorino, Boris Bessaud, Maël Tolou, Hugues Almeras, Lionel Grandadam, Marc Rolland, Dominique Peyrefitte, Christophe
Source	Elsevier; Medline; PMC
abstract	Abstract Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that causes epidemic fever, rash and polyarthralgia in Africa and Asia. Although it is known since the 1950s, new epidemiological and clinical features reported during the recent outbreak in the Indian Ocean can be regarded as the emergence of a new disease. Numerous severe forms of the infection have been described that put emphasis on the lack of efficient antiviral therapy. Among the virus-encoded enzymes, nsP2 constitutes an attractive target for the development of antiviral drugs. It is a multifunctional protein of approximately 90kDa with a helicase motif in the N-terminal portion of the protein while the papain-like protease activity resides in the C-terminal portion. The nsP2 proteinase is an essential enzyme whose proteolytic activity is critical for virus replication. In this work, a recombinant CHIKV nsP2pro and a C-terminally truncated variant were expressed in Escherichia coli and purified by metal–chelate chromatography. The enzymatic properties of the proteinase were then determined using specific synthetic fluorogenic substrates. This study constitutes the first characterization of a recombinant CHIKV nsP2 cysteine protease, which may be useful for future drug screening.
has issue date	2008-02-29(xsd:dateTime)
bibo:doi	10.1016/j.virusres.2007.09.009
bibo:pmid	17961784
has license	els-covid
sha1sum (hex)	d127e9fa7bcad289c4435d88c3e779ec46dd6de0
schema:url	https://doi.org/10.1016/j.virusres.2007.09.009
resource representing a document's title	Expression and biochemical characterization of nsP2 cysteine protease of Chikungunya virus
has PubMed Central identifier	PMC7114110
has PubMed identifier	17961784
schema:publication	Virus Research
resource representing a document's body	covid:d127e9fa7bcad289c4435d88c3e779ec46dd6de0#body_text
is schema:about of	named entity 'HELICASE' named entity 'EPIDEMIC' named entity 'SEVERE' named entity 'ENZYME' named entity 'RECENT' named entity 'DESCRIBED' named entity 'N-TERMINAL ' named entity 'DISEASE' named entity 'DEVELOPMENT' named entity 'APPROXIMATELY' named entity 'VIRUS REPLICATION' named entity 'EFFICIENT' named entity 'ATTRACTIVE' named entity 'INDIAN OCEAN' named entity 'protease' named entity 'proteolytic' named entity 'CYSTEINE PROTEASE' named entity 'ALPHAVIRUS' named entity 'EXPRESSION' named entity 'MOSQUITO' named entity 'INFECTION' named entity 'IS A' named entity 'C-TERMINAL' named entity 'CAUSES' named entity 'TARGET' named entity 'AFRICA' named entity 'OUTBREAK' named entity 'CHIKUNGUNYA VIRUS' named entity 'NSP2' named entity 'KDA' named entity 'NSP2' named entity 'HAVE' named entity 'EMERGENCE' named entity 'CLINICAL FEATURES' named entity 'PROTEIN ' named entity 'BORNE' named entity 'NEW' named entity 'ASIA' named entity 'MOTIF' named entity 'LACK' named entity 'PROTEOLYTIC ACTIVITY' named entity 'CHIKUNGUNYA VIRUS' named entity 'BIOCHEMICAL CHARACTERIZATION' named entity 'ESSENTIAL' named entity 'ANTIVIRAL THERAPY' named entity 'EPIDEMIOLOGICAL' named entity 'CRITICAL' named entity 'ANTIVIRAL DRUGS' named entity 'FORMS' named entity 'NUMEROUS' named entity 'RASH' named entity 'POLYARTHRALGIA' named entity 'KNOWN' named entity 'ENZYMES' named entity 'VIRUS' named entity 'FEVER' named entity 'PAPAIN' named entity 'LIKE' named entity 'NSP2 PROTEINASE' named entity 'ENCODED' named entity 'TERMINAL PORTION' named entity 'PROTEASE ACTIVITY' named entity 'REPORTED' named entity 'infection' named entity 'development' named entity 'protein' named entity 'activity' named entity 'helicase' named entity 'enzymes' named entity 'antiviral drugs' named entity 'regarded' named entity 'C-terminal' named entity 'polyarthralgia' named entity 'protease' named entity 'nsP2'

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