About: The guanine nucleotide exchange factor GBF1 participates in rotavirus replication

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The guanine nucleotide exchange factor GBF1 participates in rotavirus replication
Creator	Lee, Eunjoo Sztul, Elizabeth López, Susana Silva-Ayala, Daniela Arnoldi, Francesca Eichwald, Catherine Arias, Carlos Schraner, Elisabeth Burrone, Óscar Martínez, José
Source	BioRxiv; MedRxiv
abstract	Cellular and viral factors participate in the replication cycle of rotavirus. We report that the guanine nucleotide exchange factor GBF1, which activates the small GTPase Arf1 to induce COPI transport processes, is required for rotavirus replication since knocking down GBF1 expression by RNA interference, or inhibiting its activity by treatment with Brefeldin A (BFA) or Golgicide A (GCA) significantly reduce the yield of infectious viral progeny. This reduction in virus yield was related to a block in virus assembly since in the presence of either BFA or GCA the assembly of infectious mature triple-layered virions was significantly prevented and only doubled layered-particles were detected. We report that the catalytic activity of GBF1, but not the activation of Arf1, is essential for the assembly of the outer capsid of rotavirus. We show that both BFA and GCA, as well as interfering with the synthesis of GBF1, alter the electrophoretic mobility of glycoproteins VP7 and NSP4 and block the trimerization of the virus surface VP7, a step required for its incorporation into virus particles. Although a post-translational modification of VP7 (other than glycosylation) could be related to the lack of trimerization, we found that NSP4 might also be involved in this process, since knocking-down its expression reduces VP7 trimerizarion. In support, recombinant VP7 protein overexpressed in transfected cells formed trimers only when co-transfected with NSP4. Rotavirus, a member of the family Reoviridae, is the major cause of severe diarrhea in children and young animals worldwide. Despite the significant advances in the characterization of the biology of this virus, the mechanisms involved in morphogenesis of the virus particle are still poorly understood. In this work, we show that the guanine nucleotide exchange factor GBF1, relevant for the COPI/Arf1-mediated cellular vesicular transport, participates in the replication cycle of the virus, influencing the correct processing of viral glycoproteins VP7 and NSP4, and the assembly of the virus surface proteins VP7 and VP4.
has issue date	2019-04-29(xsd:dateTime)
bibo:doi	10.1101/619924
has license	medrxiv
sha1sum (hex)	d10525ee12ac8eb0941e0b21d04b3552baaeb79d
schema:url	https://doi.org/10.1101/619924
resource representing a document's title	The guanine nucleotide exchange factor GBF1 participates in rotavirus replication
schema:publication	bioRxiv
resource representing a document's body	covid:d10525ee12ac8eb0941e0b21d04b3552baaeb79d#body_text
is schema:about of	named entity 'polymerase' named entity 'replication' named entity 'layer' named entity 'genome' named entity 'diarrhea' named entity 'replication' named entity 'segments' named entity 'VP1' named entity 'rotavirus' named entity 'VP4' named entity 'virus' named entity 'Cellular' named entity 'virus' named entity 'cellular' named entity 'virus' named entity 'small GTPase' named entity 'composed' named entity 'transferase' named entity 'host' named entity 'genome' named entity 'TLPs' named entity 'virus particle' named entity 'VP6' named entity 'COPI' named entity 'glycoproteins' named entity 'RNA dependent RNA polymerase' named entity 'VP7' named entity 'rotavirus' named entity 'VP1' named entity 'guanine nucleotide exchange factor' named entity 'guanylyl' named entity 'Reoviridae' named entity 'vesicular' named entity 'cytoplasmic' named entity 'VP7' named entity 'rotavirus' named entity 'VP7' named entity 'severe diarrhea' named entity 'NSP5' named entity 'nucleotide exchange factor' named entity 'glycoprotein' named entity 'rotavirus' named entity 'GBF1' named entity 'GBF1' named entity 'double-stranded' named entity 'GBF1' named entity '1, 2' named entity 'spike protein' named entity 'NSP4' named entity 'RNA' named entity 'nucleotide exchange factor' named entity 'dsRNA' named entity 'replication' named entity 'VP7' named entity 'spike' named entity 'viroplasms' named entity 'eleven' named entity 'Rotavirus' named entity 'replication cycle' named entity 'dsRNA' named entity 'VP6' named entity 'rotavirus' named entity 'guanine nucleotide exchange factor' named entity 'replication cycle' named entity 'GBF1' named entity 'VP7' named entity 'COPI' named entity 'Reoviridae' named entity 'non-enveloped' named entity 'virus' named entity 'NSP2' named entity 'viral genome' named entity 'genome' named entity 'methyl transferase' named entity 'Arf1'

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