About: T cell-mediated hepatitis in mice infected with lymphocytic choriomeningitis virus. Liver cell destruction by H-2 class I- restricted virus-specific cytotoxic T cells as a physiological correlate of the 51Cr-release assay?

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About: T cell-mediated hepatitis in mice infected with lymphocytic choriomeningitis virus. Liver cell destruction by H-2 class I- restricted virus-specific cytotoxic T cells as a physiological correlate of the 51Cr-release assay? Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	T cell-mediated hepatitis in mice infected with lymphocytic choriomeningitis virus. Liver cell destruction by H-2 class I- restricted virus-specific cytotoxic T cells as a physiological correlate of the 51Cr-release assay?
Creator	Hengartner, Hans Zinkernagel, Rolf Cerny, Andreas Althage, Alana Haenseler, Edi Leist, Thomas
Source	PMC
abstract	A model for immunologically T cell-mediated hepatitis was established in mice infected with lymphocytic choriomeningitis virus (LCMV). The severity of hepatitis was monitored histologically and by determination of changes in serum levels of the enzymes alanine aminotransferase (ALT), aspartate aminotransferase (AST), glutamate dehydrogenase (GLDH), and alkaline phosphatase (AP). Kinetics of histological disease manifestations, increases of liver enzyme levels in the serum, and cytotoxic T cell activities in livers and spleens all correlated and were dependent upon several parameters: LCMV-isolate; LCMV-WE caused extensive hepatitis, LCMV-Armstrong virtually none. Virus dose. Route of infection; i.v. or i.p. infection caused hepatitis, whereas infection into the footpad did not. The general genetic background of the murine host; of the strains tested, Swiss mice and A-strain mice were more susceptible than C57BL or CBA mice; BALB/c and DBA/2 mice were least susceptible. The degree of immunocompetence of the murine host; T cell deficient nu/nu mice never developed hepatitis, whereas nu/+ or +/+ mice always did. B cell-depleted anti-IgM-treated mice developed immune-mediated hepatitis comparably or even more extensively than control mice. Local cytotoxic T cell activity; mononuclear cells isolated from livers during the period of overt hepatitis were two to five times more active than equal numbers of spleen cells. Adoptive transfer of nylon wool-nonadherent anti-Thy-1.2 and anti-Lyt-2 plus C- sensitive, anti-L3T4 plus C-resistant lymphocytes into irradiated mice preinfected with LCMV-WE caused a rapid time- and dose-dependent linear increase of serum enzyme levels. This increase was caused by adoptive transfer of lymphocytes if immune cell donors and recipient mice shared class I, but not when they shared class II histocompatibility antigens. The donor cell dose-dependent increase of these enzymes was first measurable 6-18 h after transfer with 2 X 10(8) cells or 3 X 10(6) cells, respectively. The time-dependent increase caused by the adoptive transfer of 1-2 X 10(8) cells was strictly linear during a period of up to 25-40 h. These results indicate single-hit kinetics of liver cell death and suggest that effector T cells destroy infected liver cells via direct contact rather than via soluble toxic mediators. The results may represent the best in vivo correlate of the in vitro 51Cr-release assay that has been analyzed so far, and strongly support the view that antiviral cytotoxic T cells are directly cytolytic in vivo.(ABSTRACT TRUNCATED AT 400 WORDS)
has issue date	1986-10-01(xsd:dateTime)
bibo:pmid	3489805
has license	cc-by-nc-sa
sha1sum (hex)	d0cd535962322457ab99af525bb76af8f5cbe3e1
resource representing a document's title	T cell-mediated hepatitis in mice infected with lymphocytic choriomeningitis virus. Liver cell destruction by H-2 class I- restricted virus-specific cytotoxic T cells as a physiological correlate of the 51Cr-release assay?
has PubMed Central identifier	PMC2188412
has PubMed identifier	3489805
schema:publication	J Exp Med
resource representing a document's body	covid:d0cd535962322457ab99af525bb76af8f5cbe3e1#body_text
is schema:about of	named entity 'Correlate' named entity 'LYMPHOCYTIC CHORIOMENINGITIS VIRUS' named entity 'Cytotoxic' named entity 'LCMV' named entity 'aspartate aminotransferase' named entity 'Hornby, Ontario' named entity 'irradiation' named entity 'enzyme' named entity 'enzyme levels' named entity 'spleen' named entity 'serum' named entity 'titer' named entity 'Canada' named entity 'histological' named entity 'LCMV' named entity 'carrier status' named entity 'genetic background' named entity 'linear time' named entity 'infection' named entity 'C57BL/6' named entity '1, 2' named entity 'LCMV' named entity 'infection' named entity 'effector T cells' named entity 'tible' named entity 'immunosuppressed' named entity 'serum' named entity 'hepatitis' named entity 'cultured cells' named entity 'clinical symptoms' named entity 'LCMV' named entity 'Liver Disease' named entity 'cytotoxic T cell' named entity 'liver cell' named entity 'virus' named entity 'cytotoxic T cells' named entity 'antibodies' named entity 'dose dependent' named entity 'hepatitis' named entity 'cellular functions' named entity 'IgM' named entity 'liver cells' named entity 'target cells' named entity 'Berlin' named entity 'hepatitis' named entity 'AST' named entity 'Mononuclear cells' named entity 'effector T cells' named entity 'peripheral blood' named entity 'Vaccinia virus' named entity 'pathology' named entity 'lysis' named entity 'Lymphocytes' named entity 'Virus' named entity 'LCMV' named entity 'hepatitis' named entity 'LCMV' named entity 'murine' named entity 'eclipse phase' named entity 'murine tumor' named entity 'assay' named entity 'enzyme' named entity 'LCMV' named entity 'major histocompatibility' named entity 'tumor cells' named entity 'Cytotoxic activity' named entity 'hepatitis' named entity 'serum' named entity 'hepatitis' named entity 'serum' named entity 'Infection' named entity 'enzyme' named entity 'serum' named entity 'lymphocytes' named entity 'surface antigens' named entity 'lymphocytes' named entity 'LCMV' named entity 'C57BL/6'

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