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About:
Optimization of Codon Usage of Poxvirus Genes allows for Improved Transient Expression in Mammalian Cells
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schema:ScholarlyArticle
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covidontheweb.inria.fr
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document(s)
Type:
Academic Article
research paper
schema:ScholarlyArticle
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type
Academic Article
research paper
schema:ScholarlyArticle
isDefinedBy
Covid-on-the-Web dataset
has title
Optimization of Codon Usage of Poxvirus Genes allows for Improved Transient Expression in Mammalian Cells
Creator
Barrett, John
Mcfadden, Grant
Ae, Craig
Ae, Steven
Ae, Sun
Ae, Tara
Barrett, J
Belsito, Ae
Brunetti, C
Mcfadden, Ae
Nazarian, Ae
Sun, Ae
Yunming, A
Source
Medline; PMC
abstract
Transient expression of viral genes from certain poxviruses in uninfected mammalian cells can sometimes be unexpectedly inefficient. The reasons for poor expression levels can be due to a number of features of the gene cassette, such as cryptic splice sites, polymerase II termination sequences or motifs that lead to mRNA instability. Here we suggest that in some cases the problem of low protein expression in transfected mammalian cells may be due to inefficient codon usage. We have observed that for many poxvirus genes from the yatapoxvirus genus this deficiency can be overcome by synthesis of the gene with codon sequences optimized for expression in primate cells. This led us to examine colon usage across 2-dozen sequenced members of the Poxviridae. We conclude that codon usage is surprisingly divergent across the different Poxviridae genera but is much more conserved within a single genus. Thus, Poxviridae genera can be divided into distinct groups based on their observed codon bias. When viewed in this context, successful transient expression of transfected poxvirus genes in uninfected mammalian cells can be more accurately predicted based on codon bias. As a corollary, for specific poxvirus genes with less favorable codon usage, codon optimization can result in profoundly increased transient expression levels following transfection of uninfected mammalian cell lines.
has issue date
2006-01-01
(
xsd:dateTime
)
bibo:doi
10.1007/s11262-005-0035-7
bibo:pmid
16791414
has license
no-cc
sha1sum (hex)
d09ecfd5cef1bcb5dff8519888d9064a85dc71e3
schema:url
https://doi.org/10.1007/s11262-005-0035-7
resource representing a document's title
Optimization of Codon Usage of Poxvirus Genes allows for Improved Transient Expression in Mammalian Cells
has PubMed Central identifier
PMC7089053
has PubMed identifier
16791414
schema:publication
Virus Genes
resource representing a document's body
covid:d09ecfd5cef1bcb5dff8519888d9064a85dc71e3#body_text
is
schema:about
of
named entity 'problem'
named entity 'genes'
named entity 'expression'
named entity 'observed'
named entity 'lead'
named entity 'optimized'
named entity 'groups'
named entity 'CODON USAGE'
named entity 'POXVIRUS'
named entity 'TO EXAMINE'
named entity 'POXVIRUSES'
named entity 'LOW PROTEIN'
named entity 'CONSERVED'
named entity 'DIFFERENT'
named entity 'MRNA INSTABILITY'
named entity 'HAVE'
named entity 'GENES'
named entity 'DUE TO'
named entity 'CELLS'
named entity 'SPECIFIC'
named entity 'EXPRESSION'
named entity 'CODON BIAS'
named entity 'MEMBERS'
named entity 'PRIMATE'
named entity 'COLON'
named entity 'TRANSFECTION'
named entity 'VIRAL GENES'
named entity 'MAMMALIAN CELL'
named entity 'MAY BE'
named entity 'GENE CASSETTE'
named entity 'PROBLEM'
named entity 'UNINFECTED'
named entity 'TRANSIENT EXPRESSION'
named entity 'YATAPOXVIRUS'
named entity 'FOLLOWING'
named entity 'GENES'
named entity 'PROTEIN EXPRESSION'
named entity 'GENE'
named entity 'IMPROVED'
named entity 'TRANSIENT EXPRESSION'
named entity 'MAMMALIAN'
named entity 'ALLOWS'
named entity 'OPTIMIZATION'
named entity 'DEFICIENCY'
named entity 'POXVIRUS'
named entity 'CERTAIN'
named entity 'MUCH'
named entity 'BUT'
named entity 'USAGE'
named entity 'NUMBER OF'
named entity 'GROUPS'
named entity 'TERMINATION'
named entity 'LED'
named entity 'CONTEXT'
named entity 'HERE'
named entity 'MAMMALIAN'
named entity 'POLYMERASE'
named entity 'RESULT'
named entity 'CODON USAGE'
named entity 'DISTINCT'
named entity 'SYNTHESIS'
named entity 'FEATURES'
named entity 'CODON'
named entity 'POXVIRIDAE'
named entity 'THEIR'
named entity 'LEVELS'
named entity 'ACROSS'
named entity 'INCREASED'
named entity 'CELLS'
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