About: A small stem-loop structure of the Ebola virus trailer is essential for replication and interacts with heat-shock protein A8

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: A small stem-loop structure of the Ebola virus trailer is essential for replication and interacts with heat-shock protein A8 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A small stem-loop structure of the Ebola virus trailer is essential for replication and interacts with heat-shock protein A8
Creator	Johnson, Reed Palacios, Gustavo Wiley, Michael Sztuba-Solinska, Joanna Kumar, Mia Diaz, Larissa Jozwick, Lucas Kolb, Gaëlle Kuhn, Jens Radoshitzky, Sheli Le Grice, Stuart
source	Medline; PMC
abstract	Ebola virus (EBOV) is a single-stranded negative-sense RNA virus belonging to the Filoviridae family. The leader and trailer non-coding regions of the EBOV genome likely regulate its transcription, replication, and progeny genome packaging. We investigated the cis-acting RNA signals involved in RNA–RNA and RNA–protein interactions that regulate replication of eGFP-encoding EBOV minigenomic RNA and identified heat shock cognate protein family A (HSC70) member 8 (HSPA8) as an EBOV trailer-interacting host protein. Mutational analysis of the trailer HSPA8 binding motif revealed that this interaction is essential for EBOV minigenome replication. Selective 2′-hydroxyl acylation analyzed by primer extension analysis of the secondary structure of the EBOV minigenomic RNA indicates formation of a small stem-loop composed of the HSPA8 motif, a 3′ stem-loop (nucleotides 1868–1890) that is similar to a previously identified structure in the replicative intermediate (RI) RNA and a panhandle domain involving a trailer-to-leader interaction. Results of minigenome assays and an EBOV reverse genetic system rescue support a role for both the panhandle domain and HSPA8 motif 1 in virus replication.
has issue date	2016-11-16(xsd:dateTime)
bibo:doi	10.1093/nar/gkw825
bibo:pmid	27651462
has license	no-cc
sha1sum (hex)	d07a12c15f26f94f6b1d0fc1ab61730140e8c7d2
schema:url	https://doi.org/10.1093/nar/gkw825
resource representing a document's title	A small stem-loop structure of the Ebola virus trailer is essential for replication and interacts with heat-shock protein A8
has PubMed Central identifier	PMC5175359
has PubMed identifier	27651462
schema:publication	Nucleic Acids Res
resource representing a document's body	covid:d07a12c15f26f94f6b1d0fc1ab61730140e8c7d2#body_text
is schema:about of	named entity 'TRAILER' named entity 'TRAILER' named entity 'REVERSE' named entity 'RESULTS' named entity 'SMALL' named entity 'INVOLVING' named entity 'ANALYZED' named entity 'COMPOSED OF' named entity 'REPLICATION' named entity 'CIS' named entity 'HYDROXYL ' named entity 'SIMILAR' named entity 'EGFP' named entity 'heat shock' named entity 'replication' named entity 'composed' named entity 'panhandle' named entity 'primer extension' named entity 'cognate' named entity 'intermediate' named entity 'acylation' named entity 'heat-shock protein' named entity 'Ebola virus' named entity 'structure' named entity 'ACTING' named entity 'REPLICATION' named entity 'EBOLA VIRUS' named entity 'PROTEIN ' named entity 'SELECTIVE' named entity 'FORMATION' named entity 'CODING' named entity 'REGULATE' covid:arg/d07a12c15f26f94f6b1d0fc1ab61730140e8c7d2 named entity 'HEAT-SHOCK PROTEIN' named entity 'ENCODING' named entity 'ESSENTIAL' named entity 'SIGNALS' named entity 'PRIMER EXTENSION ANALYSIS' named entity 'VIRUS REPLICATION' named entity 'SECONDARY STRUCTURE' named entity 'IDENTIFIED' named entity 'STEM-LOOP' named entity 'HOST PROTEIN' named entity 'PACKAGING' named entity 'PREVIOUSLY' named entity 'PANHANDLE' named entity 'PROGENY' named entity 'GENOME' named entity 'INTERACTING' named entity 'INVOLVED' named entity 'STRUCTURE' named entity 'MEMBER' named entity 'HEAT SHOCK' named entity 'REGIONS' named entity 'EBOV' named entity 'INTERMEDIATE' named entity 'HSPA8' named entity '1868' named entity 'ITS' named entity 'REVEALED' named entity 'INTERACTION' named entity 'SUPPORT' named entity 'PROTEIN INTERACTIONS' named entity 'RESCUE' named entity 'DOMAIN' named entity 'ESSENTIAL' named entity 'SMALL' named entity 'STEM-LOOP STRUCTURE' named entity 'MUTATIONAL ANALYSIS' named entity 'RNA' named entity 'TRANSCRIPTION'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software