About: Rapid Transient Production of a Monoclonal Antibody Neutralizing the Porcine Epidemic Diarrhea Virus (PEDV) in Nicotiana benthamiana and Lactuca sativa

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About: Rapid Transient Production of a Monoclonal Antibody Neutralizing the Porcine Epidemic Diarrhea Virus (PEDV) in Nicotiana benthamiana and Lactuca sativa Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Rapid Transient Production of a Monoclonal Antibody Neutralizing the Porcine Epidemic Diarrhea Virus (PEDV) in Nicotiana benthamiana and Lactuca sativa
Source	Medline; PMC
abstract	Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea, vomiting, dehydration, weight loss, and high mortality rate in neonatal piglets. Porcine epidemic diarrhea (PED) has been reported in Europe, America, and Asia including Thailand. The disease causes substantial losses to the swine industry in many countries. Presently, there is no effective PEDV vaccine available. In this study, we developed a plant-produced monoclonal antibody (mAb) 2C10 as a prophylactic candidate to prevent the PEDV infection. Recently, plant expression systems have gained interest as an alternative for the production of antibodies because of many advantages, such as low production cost, lack of human and animal pathogen, large scalability, etc. The 2C10 mAb was transiently expressed in Nicotiana benthamiana and lettuce using geminiviral vector. After purification by protein A affinity chromatography, the antibody was tested for the binding and neutralizing activity against PEDV. Our result showed that the plant produced 2C10 mAb can bind to the virus and also inhibit PEDV infection in vitro . These results show excellent potential for a plant-expressed 2C10 as a PEDV prophylaxis and a diagnostic for PEDV infection.
has issue date	2017-06-02(xsd:dateTime)
bibo:doi	10.1055/s-0043-112344
bibo:pmid	28575911
has license	no-cc
sha1sum (hex)	cf44177a07bf13f4ee0ffd4a19d99fee471fe91b
schema:url	https://doi.org/10.1055/s-0043-112344
resource representing a document's title	Rapid Transient Production of a Monoclonal Antibody Neutralizing the Porcine Epidemic Diarrhea Virus (PEDV) in Nicotiana benthamiana and Lactuca sativa
has PubMed Central identifier	PMC7117083
has PubMed identifier	28575911
schema:publication	Planta Med
resource representing a document's body	covid:cf44177a07bf13f4ee0ffd4a19d99fee471fe91b#body_text
is schema:about of	named entity 'alkaloids' named entity 'TCID50' named entity 'GenBank' named entity 'infection' named entity 'antibody' named entity 'diarrhea' named entity 'IgG' named entity 'protease' named entity 'Vero cell' named entity 'antibodies' named entity 'virus' named entity 'cytopathic effect' named entity 'oral vaccine' named entity 'pathogen' named entity 'IgG' named entity 'mAb' named entity 'viral vectors' named entity 'heavy chain' named entity 'western blot' named entity 'antibodies' named entity 'mAb' named entity 'antiserum' named entity 'lettuce' named entity 'pathogen' named entity 'antibody' named entity 'Escherichia coli' named entity 'peptides' named entity 'nucleotide sequences' named entity 'milk' named entity 'protein' named entity 'grocery store' named entity 'antibodies' named entity 'Lactuca sativa' named entity 'PEDV' named entity 'N. benthamiana' named entity 'passive immunity' named entity 'dehydration' named entity 'gene silencing' named entity 'vaccine' named entity 'mAb' named entity 'Chulalongkorn University' named entity 'vaccine' named entity 'light chains' named entity 'PEDV' named entity 'SDS-PAGE' named entity 'phenolics' named entity 'lettuce' named entity 'w/v' named entity 'Vero cells' named entity 'recombinant proteins' named entity 'N. benthamiana' named entity 'standard deviation' named entity 'negative control' named entity 'IgG' named entity 'lettuce' named entity 'primers' named entity 'viral vectors' named entity 'protein' named entity 'kDa' named entity 'heavy chain' named entity 'watery diarrhea' named entity 'N. benthamiana' named entity 'agroinfiltration' named entity 'PCR' named entity 'skim milk' named entity 'virus' named entity 'negative control' named entity 'neonatal' named entity 'protective antibodies' named entity 'Agrobacterium' named entity 'virus' named entity '37°C' named entity 'vaccination' named entity 'infection' named entity 'piglet' named entity 'lettuce' named entity 'N-terminus' named entity 'higher concentration' named entity 'Porcine' named entity 'Vero cells' named entity 'replicon' named entity 'antibodies'

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