About: Identification of single-chain antibody fragments specific against SARS-associated coronavirus from phage-displayed antibody library

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About: Identification of single-chain antibody fragments specific against SARS-associated coronavirus from phage-displayed antibody library Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Identification of single-chain antibody fragments specific against SARS-associated coronavirus from phage-displayed antibody library
Creator	Qian, Juan Du, En-Qi Ling, Wei-Fang Liu, Ying-Le Liu, Zheng-Xue Qi, Yi-Peng Yan, Jun-Peng Yi, Guo-Hua
Source	Elsevier; Medline; PMC
abstract	Abstract To develop early diagnostic reagents, effective vaccines, and even drugs against SARS-associated coronavirus (SARS-CoV), the human single fold single-chain antibody fragments, (scFv) libraries I+J (Tomlinson I+J) were used to identify novel scFvs, which can specifically bind to SARS-CoV. Interestingly, two scFvs (B5 and B9) exhibited higher binding specificity to SARS-CoV with the OD450 value 0.608 and 0.545, respectively, and their coding sequences shared the identical sequence composed of VH gene (351bp) and VL gene (327bp), so the two scFvs were uniformly named as SA59B and chosen for further analysis. SA59B scFv was expressed in soluble form in Escherichia coli HB2151 and purified by immobilized metal affinity chromatography. The soluble 30kDa SA59B scFv-antibody was verified in SDS–PAGE and Western-blot. The purified SA59B scFv-antibody was labeled with HRP by the glutaraldehyde method, and the concentration of HRP and SA59B scFv-antibody in the SA59B-HRP solution reached 2.4 and 2.28mg/ml, respectively. Then, the binding ability of SA59B-HRP to SARS-CoV was evaluated by ELISA with S/N of 11.6, indicating higher binding specificity between them. Finally, both the SA59B sequence specificity and its application for diagnosis, prophylaxis or therapy of SARS were discussed.
has issue date	2005-04-08(xsd:dateTime)
bibo:doi	10.1016/j.bbrc.2005.02.003
bibo:pmid	15737606
has license	els-covid
sha1sum (hex)	cf2e57d07cee8edf7204b1e0b7c80818995cbed8
schema:url	https://doi.org/10.1016/j.bbrc.2005.02.003
resource representing a document's title	Identification of single-chain antibody fragments specific against SARS-associated coronavirus from phage-displayed antibody library
has PubMed Central identifier	PMC7092915
has PubMed identifier	15737606
schema:publication	Biochemical and Biophysical Research Communications
resource representing a document's body	covid:cf2e57d07cee8edf7204b1e0b7c80818995cbed8#body_text
is schema:about of	named entity 'develop' named entity 'higher' named entity 'diagnosis' named entity 'indicating' named entity 'SDS-PAGE' named entity 'specificity' named entity 'Identification' named entity 'specific' named entity 'antibody' named entity 'specificity' named entity 'shared' named entity 'kDa' named entity 'gene' named entity 'therapy' named entity 'binding' named entity 'immobilized' named entity 'effective' named entity 'fragments' named entity 'specificity' named entity 'composed' named entity 'HRP' named entity 'single-chain' named entity 'SDS-PAGE' named entity 'SARS' named entity 'SARS-CoV' named entity 'vaccines' named entity 'gene' named entity 'antibody' named entity 'immobilized metal affinity chromatography' named entity 'coding sequences' named entity 'single-chain' named entity 'scFv' named entity 'scFv' named entity 'glutaraldehyde' named entity 'prophylaxis' named entity 'kDa' named entity 'identical' named entity 'sequence' named entity 'gene' named entity 'Western-blot' named entity 'HRP' named entity 'scFvs' named entity 'phage' named entity 'SARS-CoV' named entity 'scFvs' named entity 'S/N' named entity 'scFvs' named entity 'SARS-associated coronavirus' named entity 'antibody' named entity 'SARS-CoV' named entity 'antibody' named entity 'Escherichia coli' named entity '11.6' named entity 'SARS-CoV' named entity 'SARS-associated coronavirus' named entity 'HRP' named entity 'antibody' named entity 'binding' named entity 'libraries' named entity 'reagents' named entity 'HRP' named entity 'scFv' named entity 'purified' named entity 'bind' named entity 'metal' named entity 'glutaraldehyde' named entity 'concentration' named entity 'labeled' named entity 'solution' named entity 'reached' named entity 'expressed' named entity 'evaluated' named entity 'Tomlinson' named entity 'antibody'

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