About: Identification of residues on human receptor DPP4 critical for MERS-CoV binding and entry

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About: Identification of residues on human receptor DPP4 critical for MERS-CoV binding and entry Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Identification of residues on human receptor DPP4 critical for MERS-CoV binding and entry
Creator	Shi, Xuanling Wang, Dongli Wang, Ying Wang, Nianshuang Song, Wenfei Fu, Lili Guo, Jianying
Source	Elsevier; Medline; PMC
abstract	Abstract Middle East respiratory syndrome coronavirus (MERS-CoV) infects host cells through binding the receptor binding domain (RBD) on its spike glycoprotein to human receptor dipeptidyl peptidase 4 (hDPP4). Here, we report identification of critical residues on hDPP4 for RBD binding and virus entry through analysis of a panel of hDPP4 mutants. Based on the RBD–hDPP4 crystal structure we reported, the mutated residues were located at the interface between RBD and hDPP4, which potentially changed the polarity, hydrophobic or hydrophilic properties of hDPP4, thereby interfering or disrupting their interaction with RBD. Using surface plasmon resonance (SPR) binding analysis and pseudovirus infection assay, we showed that several residues in hDPP4–RBD binding interface were important on hDPP4–RBD binding and viral entry. These results provide atomic insights into the features of interactions between hDPP4 and MERS-CoV RBD, and also provide potential explanation for cellular and species tropism of MERS-CoV infection.
has issue date	2014-12-31(xsd:dateTime)
bibo:doi	10.1016/j.virol.2014.10.006
bibo:pmid	25461530
has license	els-covid
sha1sum (hex)	cf2b21513ebe7ddeb39e48f09f3f3e6bc47b117e
schema:url	https://doi.org/10.1016/j.virol.2014.10.006
resource representing a document's title	Identification of residues on human receptor DPP4 critical for MERS-CoV binding and entry
has PubMed Central identifier	PMC7112127
has PubMed identifier	25461530
schema:publication	Virology
resource representing a document's body	covid:cf2b21513ebe7ddeb39e48f09f3f3e6bc47b117e#body_text
is schema:about of	named entity 'CRITICAL' named entity 'pseudovirus' named entity 'RBD' named entity 'interface' named entity 'RBD' named entity 'SPIKE GLYCOPROTEIN' named entity 'MERS-COV' named entity 'POTENTIAL' named entity 'CELLULAR' named entity 'RECEPTOR BINDING' named entity 'MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS' named entity 'THESE' named entity 'IDENTIFICATION' named entity 'THEIR' named entity 'ATOMIC' named entity 'BASED' named entity 'RECEPTOR' named entity 'CRITICAL' named entity 'DOMAIN' named entity 'IMPORTANT' named entity 'PANEL' named entity 'VIRUS ENTRY' named entity 'HUMAN' named entity 'IDENTIFICATION' named entity 'DPP4' named entity 'BINDING' named entity 'ENTRY' named entity 'ASSAY' named entity 'EXPLANATION' named entity 'INFECTS' named entity 'HERE' named entity 'HYDROPHILIC' named entity 'RBD' named entity 'PROPERTIES' named entity 'SPECIES' named entity 'BINDING ANALYSIS' named entity 'ANALYSIS' named entity 'REPORT' named entity 'REPORTED' named entity 'INTERFACE' named entity 'FEATURES' named entity 'HDPP4' named entity 'CHANGED' named entity 'INFECTION' named entity 'HOST CELLS' named entity 'BINDING' named entity 'INTERFERING' named entity 'HUMAN' named entity 'INTERACTION' named entity 'PROVIDE' named entity 'DIPEPTIDYL PEPTIDASE 4' named entity 'DISRUPTING' named entity 'INTERACTIONS' named entity 'TROPISM' named entity 'VIRAL ENTRY' named entity 'RESULTS' named entity 'RECEPTOR' named entity 'CRYSTAL STRUCTURE' named entity 'HYDROPHOBIC' named entity 'INSIGHTS' named entity 'PSEUDOVIRUS' named entity 'MUTATED' named entity 'MUTANTS' named entity 'ITS' named entity 'USING' named entity 'SURFACE PLASMON RESONANCE' named entity 'POLARITY' named entity 'human' named entity 'surface plasmon' named entity 'interaction' named entity 'changed' named entity 'cellular' named entity 'spike' named entity 'receptor' named entity 'virus entry'

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