About: Bat Airway Epithelial Cells: A Novel Tool for the Study of Zoonotic Viruses

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About: Bat Airway Epithelial Cells: A Novel Tool for the Study of Zoonotic Viruses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Bat Airway Epithelial Cells: A Novel Tool for the Study of Zoonotic Viruses
Creator	Drosten, Christian Oppong, Samuel Wollny, Robert Eckerle, Isabella Tschapka, Marco Ehlen, Lukas Kallies, René Cottontail, Veronika Corman, Victor Mü Ller, Marcel
Source	Medline; PMC
abstract	Bats have been increasingly recognized as reservoir of important zoonotic viruses. However, until now many attempts to isolate bat-borne viruses in cell culture have been unsuccessful. Further, experimental studies on reservoir host species have been limited by the difficulty of rearing these species. The epithelium of the respiratory tract plays a central role during airborne transmission, as it is the first tissue encountered by viral particles. Although several cell lines from bats were established recently, no well-characterized, selectively cultured airway epithelial cells were available so far. Here, primary cells and immortalized cell lines from bats of the two important suborders Yangochiroptera and Yinpterochiroptera, Carollia perspicillata (Seba's short-tailed bat) and Eidolon helvum (Straw-colored fruit bat), were successfully cultured under standardized conditions from both fresh and frozen organ specimens by cell outgrowth of organ explants and by the use of serum-free primary cell culture medium. Cells were immortalized to generate permanent cell lines. Cells were characterized for their epithelial properties such as expression of cytokeratin and tight junctions proteins and permissiveness for viral infection with Rift-Valley fever virus and vesicular stomatitis virus Indiana. These cells can serve as suitable models for the study of bat-borne viruses and complement cell culture models for virus infection in human airway epithelial cells.
has issue date	2014-01-13(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0084679
bibo:pmid	24454736
has license	cc-by
sha1sum (hex)	cee3dd6c1927b863e94e9493295d99213401f158
schema:url	https://doi.org/10.1371/journal.pone.0084679
resource representing a document's title	Bat Airway Epithelial Cells: A Novel Tool for the Study of Zoonotic Viruses
has PubMed Central identifier	PMC3890267
has PubMed identifier	24454736
schema:publication	PLoS One
resource representing a document's body	covid:cee3dd6c1927b863e94e9493295d99213401f158#body_text
is schema:about of	named entity 'limited' named entity 'virus' named entity 'viral' named entity 'conditions' named entity 'cultured' named entity 'Seba' named entity 'viruses' named entity 'Epithelial' named entity 'Viruses' named entity 'Novel' named entity 'VIRUSES' named entity 'TOOL' named entity 'TISSUE' named entity 'CELL CULTURE' named entity 'EPITHELIUM' named entity 'RIFT' named entity 'PRIMARY CELLS' named entity 'IMMORTALIZED' named entity 'AVAILABLE' named entity 'ESTABLISHED' named entity 'PERMISSIVENESS' named entity 'CENTRAL' named entity 'HUMAN' named entity 'CYTOKERATIN' named entity 'RESPIRATORY TRACT' named entity 'EXPRESSION' named entity 'PROPERTIES' named entity 'TIGHT JUNCTIONS' named entity 'BATS' named entity 'CHARACTERIZED' named entity 'TAILED' named entity 'AIRWAY' covid:arg/cee3dd6c1927b863e94e9493295d99213401f158 named entity 'FROZEN' named entity 'EPITHELIAL' named entity 'CAROLLIA PERSPICILLATA' named entity 'VIRUS' named entity 'CELLS' named entity 'RESERVOIR' named entity 'GENERATE' named entity 'CULTURE MEDIUM' named entity 'ISOLATE' named entity 'SPECIES' named entity 'STUDY' named entity 'COMPLEMENT' named entity 'RECENTLY' named entity 'HAVE' named entity 'AIRBORNE TRANSMISSION' named entity 'ZOONOTIC' named entity 'NOVEL' named entity 'BORNE' named entity 'THEIR' named entity 'PLAYS' named entity 'DIFFICULTY' named entity 'UNSUCCESSFUL' named entity 'FREE' named entity 'SPECIMENS' named entity 'INDIANA' named entity 'STUDY' named entity 'THESE' named entity 'HOST SPECIES' named entity 'FAR' named entity 'LIMITED BY' named entity 'EIDOLON HELVUM' named entity 'SHORT' named entity 'ZOONOTIC' named entity 'PRIMARY CELL CULTURE' named entity '27S' named entity 'PERMANENT' named entity 'EPITHELIAL CELLS' named entity 'FRUIT' named entity 'VIRAL INFECTION'

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