About: Initial events in bovine coronavirus infection: analysis through immunogold probes and lysosomotropic inhibitors

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About: Initial events in bovine coronavirus infection: analysis through immunogold probes and lysosomotropic inhibitors Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Initial events in bovine coronavirus infection: analysis through immunogold probes and lysosomotropic inhibitors
Creator	Storz, J Payne, H Henk, W
Source	Medline; PMC
abstract	The early events in the infection of human rectal tumor cells by bovine coronavirus were investigated by colloidal gold-mediated immunoelectron microscopy and by analysis of the effect of lysosomotropic weak bases on virus yield. Electron microscopic studies revealed sites of fusion between the virus envelope and the plasmalemma but fusion events along intracellular membranes were not observed despite extensive searches. Virion-antibody-colloidal gold complexes were, in fact, endocytosed by synchronously infected cells. These complexes were apparently non-infectious, and they accumulated in vacuoles that resembled secondary lysosomes. Exposure of cells to ammonium chloride or to methylamine during the first hour of infection had little inhibitory effect on the production of infectious virus. Chloroquine treatments were inhibitory but this effect depended on relatively late events in the infectious process. The chloroquine inhibitory step blocked infection of virus adsorbed to cells that were exposed to buffers in the pH range of 4.4 to 8.4. These findings indicate that BCV penetrates its host cell by direct fusion with the plasmalemma and does not require an acidic intracellular compartment for infectious entry.
has issue date	1990-01-01(xsd:dateTime)
bibo:doi	10.1007/bf01310747
bibo:pmid	2173524
has license	no-cc
sha1sum (hex)	cd692adc9452c7d43de9717ef83ddbde74dffb76
schema:url	https://doi.org/10.1007/bf01310747
resource representing a document's title	Initial events in bovine coronavirus infection: analysis through immunogold probes and lysosomotropic inhibitors
has PubMed Central identifier	PMC7086846
has PubMed identifier	2173524
schema:publication	Arch Virol
resource representing a document's body	covid:cd692adc9452c7d43de9717ef83ddbde74dffb76#body_text
is schema:about of	named entity 'virus' named entity 'process' named entity 'lysosomes' named entity 'accumulated' named entity 'These' named entity 'ammonium chloride' named entity 'chloroquine' named entity 'blocked' named entity 'BOVINE CORONAVIRUS' named entity 'CORONAVIRUS INFECTION' named entity 'TREATMENTS' named entity 'SITES' named entity 'INFECTION' named entity 'EXTENSIVE' named entity 'CELLS' named entity 'RECTAL TUMOR' named entity 'PLASMALEMMA' named entity 'INTRACELLULAR MEMBRANES' named entity 'REVEALED' named entity 'VACUOLES' named entity 'IMMUNOELECTRON MICROSCOPY' named entity 'APPARENTLY' named entity 'MICROSCOPIC' covid:arg/cd692adc9452c7d43de9717ef83ddbde74dffb76 named entity 'INHIBITORY EFFECT' named entity 'CHLOROQUINE' named entity 'COMPLEXES' named entity 'SECONDARY' named entity 'PRODUCTION' named entity 'DOES NOT' named entity 'ACCUMULATED' named entity 'PENETRATES' named entity 'COMPARTMENT' named entity 'EXPOSED TO' named entity 'FINDINGS' named entity 'BASES' named entity 'EXPOSURE' named entity 'INFECTED' named entity '4.4' named entity 'INVESTIGATED' named entity 'ELECTRON' named entity 'INFECTIOUS' named entity 'DIRECT' named entity 'BCV' named entity 'INHIBITORY' named entity 'ACIDIC' named entity 'NOT OBSERVED' named entity 'TUMOR CELLS' named entity 'FUSION' named entity 'EARLY' named entity 'INTRACELLULAR' named entity 'EVENTS' named entity 'IMMUNOGOLD' named entity 'ANALYSIS' named entity '8.4' named entity 'BUFFERS' named entity 'ENTRY' named entity 'INFECTIOUS PROCESS' named entity 'ANALYSIS' named entity 'INITIAL' named entity 'PROBES' named entity 'ITS' named entity 'PH RANGE' named entity 'AMMONIUM CHLORIDE' named entity 'THESE' named entity 'WEAK' named entity 'HOST CELL' named entity 'STEP' named entity 'BOVINE CORONAVIRUS' named entity 'LATE' named entity 'YIELD' named entity 'VIRUS' named entity 'COLLOIDAL GOLD' named entity 'STUDIES' named entity 'METHYLAMINE' named entity 'FACT' named entity 'HUMAN' named entity 'NON-' named entity 'BUT'

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