About: A direct isothermal amplification system adapted for rapid SNP genotyping of multifarious sample types

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About: A direct isothermal amplification system adapted for rapid SNP genotyping of multifarious sample types Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A direct isothermal amplification system adapted for rapid SNP genotyping of multifarious sample types
Creator	Cui, Yali Gao, Linlin Hua, Kai Hui, Wenli Li, Hang Li, Ningning Liu, Kewu Liu, Xiaonan Ma, Ting Yang, Xuemin Zhang, Chao Zhao, Mengye Zhu, Juanli
Source	Elsevier; Medline; PMC
abstract	Abstract Genotyping of single nucleotide polymorphisms (SNPs) in point-of-care (POC) settings could be further improved through simplifying the treatment of samples. In this study, we devised an accurate, rapid and easy-to-use SNP detection system based on direct loop-mediated isothermal amplification (LAMP) without DNA extraction, known as Direct-LAMP. Samples from various sources (including whole blood, dried blood spot, buccal swab and saliva), treated with NaOH, can be used directly in amplification. The turnaround time was about 30 min from sample collection to provision of results. The accuracy was evaluated by assessing the polymorphisms of methylenetetrahydrofolate reductase (MTHFR) C677T and aldehyde dehydrogenase-2 (ALDH2) Glu504Lys, which are better known for their critical role in folate and ethanol metabolism, respectively. Completely consistent genotyping results reveal that Direct-LAMP is generally concordant with sequencing. This system can serve as a very promising platform in the fields of disease predisposition, drug metabolism and personalized medicine.
has issue date	2018-09-15(xsd:dateTime)
bibo:doi	10.1016/j.bios.2018.05.021
bibo:pmid	29803102
has license	els-covid
sha1sum (hex)	ccd9d892241a45bffb05312a124883339a8377fe
schema:url	https://doi.org/10.1016/j.bios.2018.05.021
resource representing a document's title	A direct isothermal amplification system adapted for rapid SNP genotyping of multifarious sample types
has PubMed Central identifier	PMC7126597
has PubMed identifier	29803102
schema:publication	Biosensors and Bioelectronics
resource representing a document's body	covid:ccd9d892241a45bffb05312a124883339a8377fe#body_text
is schema:about of	named entity 'blood' named entity 'ADAPTED' named entity 'BASED' named entity 'ETHANOL METABOLISM' named entity 'METABOLISM ' named entity 'USED' named entity 'VARIOUS' named entity 'BUCCAL SWAB' named entity 'SERVE' named entity 'GENOTYPING' named entity 'TREATMENT' named entity 'SOURCES' named entity 'DNA EXTRACTION' named entity 'COMPLETELY' named entity 'STUDY' named entity 'SAMPLE COLLECTION' named entity 'COULD BE' named entity 'NAOH' named entity 'DRIED BLOOD SPOT' named entity 'DISEASE' named entity 'EVALUATED' named entity 'DIRECT' named entity 'ABOUT' named entity 'THEIR' named entity 'CONSISTENT' named entity 'ROLE' named entity 'TO PROVISION' named entity 'SNP DETECTION' named entity 'ACCURATE' named entity 'CRITICAL' named entity 'AMPLIFICATION' named entity 'ACCURACY' named entity 'PERSONALIZED MEDICINE' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'RAPID' named entity 'CONCORDANT' covid:arg/ccd9d892241a45bffb05312a124883339a8377fe named entity 'SNP GENOTYPING' named entity 'SYSTEM' named entity 'RESULTS' named entity 'BETTER' named entity 'SAMPLES' named entity 'REVEAL' named entity 'METHYLENETETRAHYDROFOLATE REDUCTASE' named entity '30 MIN' named entity 'SALIVA' named entity 'DRUG METABOLISM' named entity 'SYSTEM' named entity 'DIRECT' named entity 'RAPID' named entity 'KNOWN' named entity 'POINT-OF-CARE' named entity 'POLYMORPHISMS' named entity 'TIME' named entity 'SINGLE NUCLEOTIDE POLYMORPHISMS' named entity 'PLATFORM' named entity 'WHOLE BLOOD' named entity 'PREDISPOSITION' named entity 'ALDEHYDE DEHYDROGENASE' named entity 'FOLATE' named entity 'EASY' named entity 'SETTINGS' named entity 'AMPLIFICATION' named entity 'LAMP' named entity 'IMPROVED' named entity 'USE' named entity 'ASSESSING' named entity 'TYPES' named entity 'SAMPLE'

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