About: Control of the PI3K/Akt pathway by porcine reproductive and respiratory syndrome virus

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About: Control of the PI3K/Akt pathway by porcine reproductive and respiratory syndrome virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Control of the PI3K/Akt pathway by porcine reproductive and respiratory syndrome virus
Creator	Zhu, Liqian Tong, Guangzhi Tong, Wu Yu, Hai Zhou, Yanjun Morrison, Robert Shen, • Zhu, Jianping
topic	covid:cc954a90b7d4b595600da69a1afac9f8f0a7d075#this
Source	Medline; PMC
abstract	Phosphatidylinositol-3-kinase (PI3K)/Akt is an important cellular pathway that has been shown to participate in various replication steps of multiple viruses. In the present study, we compared the phosphorylation status of Akt during infection of MARC-145 cells and porcine alveolar macrophages (PAMs) with highly pathogenic PRRSV (HP-PRRSV) strain HuN4. We observed that biphasic activation of Akt was induced in at both the early stage (5, 15 and 30 min postinfection) and the late stage (12 and 24 h postinfection) of HP-PRRSV infection of MARC-145 cells, while an early-phase activation of Akt was found exclusively in virus-infected PAMs in vitro. Analysis with the PI3K-specific inhibitor LY294002 confirmed that PI3K acted as the upstream activator for the virus-induced activation of Akt. UV-irradiation-inactivated virus still induced the early event in PAMs but not in MARC-145 cells, suggesting that different mechanisms are employed for the early-stage induction of phosphorylated Akt within different cell cultures. We further demonstrated that FoxO1 and Bad, which serve as downstream targets of Akt, were phosphorylated in virus-infected MARC-145 cells. Moreover, the suppression of phosphorylated Akt with LY294002 significantly inhibited the virus-induced cytopathic effect (CPE) on MARC-145 cells, but it had a negligible effect on virus propagation. Collectively, our data provide new evidence of a novel role for the PI3K/Akt pathway in PRRSV infection of MARC-145 cells.
has issue date	2013-02-05(xsd:dateTime)
bibo:doi	10.1007/s00705-013-1620-z
bibo:pmid	23381397
has license	no-cc
sha1sum (hex)	cc954a90b7d4b595600da69a1afac9f8f0a7d075
schema:url	https://doi.org/10.1007/s00705-013-1620-z
resource representing a document's title	Control of the PI3K/Akt pathway by porcine reproductive and respiratory syndrome virus
has PubMed Central identifier	PMC7087347
has PubMed identifier	23381397
schema:publication	Arch Virol
resource representing a document's body	covid:cc954a90b7d4b595600da69a1afac9f8f0a7d075#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/cc954a90b7d4b595600da69a1afac9f8f0a7d075
is schema:about of	named entity 'macrophages' named entity 'Akt' named entity 'porcine' named entity 'data' named entity 'kinase' named entity 'PAMs' named entity 'PATHWAY' named entity 'STAGE' covid:arg/cc954a90b7d4b595600da69a1afac9f8f0a7d075 named entity 'NEW' named entity 'PROPAGATION' named entity 'HIGHLY' named entity 'SUPPRESSION' named entity 'DATA' named entity 'VIRUSES' named entity 'SPECIFIC' named entity 'INFECTION' named entity 'DOWNSTREAM' named entity 'EFFECT' named entity 'STEPS' named entity 'EVENT' named entity 'PATHWAY' named entity 'COMPARED' named entity 'CONTROL' named entity 'PORCINE' named entity 'PHOSPHORYLATION' named entity 'PI3K' named entity 'ACTIVATION' named entity 'PHOSPHATIDYLINOSITOL-3-KINASE' named entity 'OUR' named entity 'STATUS' named entity 'BIPHASIC' named entity 'LY294002' named entity 'BUT' named entity 'VIRUS' named entity 'REPLICATION' named entity 'EVIDENCE OF' named entity 'PI3K' named entity 'PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS' named entity 'EARLY STAGE' named entity 'PRRSV' named entity 'EMPLOYED' named entity '145' named entity '30 MIN' named entity 'AKT' named entity 'PRESENT' named entity 'PROVIDE' named entity 'CELL CULTURES' named entity 'INACTIVATED' named entity 'NOVEL' named entity 'PHOSPHORYLATED' named entity 'IMPORTANT' named entity 'ROLE' named entity 'ANALYSIS' named entity 'CONFIRMED' named entity 'INDUCED' named entity 'INHIBITED' named entity 'OBSERVED' named entity 'CELLULAR' named entity 'LATE STAGE' named entity 'TO PARTICIPATE' named entity 'MULTIPLE' named entity 'ACTIVATOR' named entity 'MECHANISMS' named entity 'TARGETS' named entity 'CELLS' named entity 'STRAIN' named entity 'STUDY' named entity 'CYTOPATHIC EFFECT' named entity 'PATHOGENIC' named entity 'ALVEOLAR MACROPHAGES' named entity 'INHIBITOR'

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