About: Isolation and characterization of avian coronavirus from healthy Eclectus parrots (Eclectus roratus) from Indonesia

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About: Isolation and characterization of avian coronavirus from healthy Eclectus parrots (Eclectus roratus) from Indonesia Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Isolation and characterization of avian coronavirus from healthy Eclectus parrots (Eclectus roratus) from Indonesia
Creator	Handharyani, E Poetri, O Setiyono, A Soejoedono, R Suryaman, G
topic	covid:cc03e915afeb7acfe93595b75a38f7b58bf2d752#this
Source	Medline; PMC
abstract	BACKGROUND AND AIM: Avian coronavirus has a wide range of hosts, from chickens and turkeys to wild birds. This virus causes an economically and, possibly, environmentally, important loss in the poultry industry. Therefore, research into the avian coronavirus in various species of birds is required. The Eclectus parrot (Eclectus roratus) is an endemic bird to Indonesia and Northern Australia and often kept as pets. At present, there has been limited information about avian coronavirus infection among birds. This study aimed to determine the presence of and to characterize avian coronavirus isolated from Eclectus parrots in Indonesia. MATERIALS AND METHODS: Cloacal swab samples were taken from 10 healthy Eclectus parrots (E. roratus). Each isolate was propagated into specific pathogen-free embryonated chicken eggs. The presence of avian coronavirus was determined using three sets of primers targeting the 3’ untranslated region (3’-UTR) of avian coronavirus (UTR41+/11−), the N gene of the infectious bronchitis virus (IBVN+/−), and the S1 gene of the IBV (XCE2+/XCE2−). The infectious bronchitis vaccine strain H120 was used as a positive control. Resulting positive bands were sequenced for the S1 gene. RESULTS: None of the isolates was positive for the 3’-UTR, four isolates were positive for the N gene of infectious bronchitis, and two isolates were positive for the S1 gene of the IBV. However, only one isolate (parrot/Indonesia/BX9/16) was sequenced for the partial S1 gene with primers XCE2+/XCE2−. The partial nucleotide sequence of this isolate showed 100% homology with the IBV GI-13 lineage, specifically with a field isolate of the 4/91 variant 1 Israel and the 4/91 vaccine on the hypervariable region 3 site of the S1 gene. CONCLUSION: An IB-like avian coronavirus was isolated from healthy Eclectus parrots. Our results indicate that IBV has a wide range of hosts, which prompt the need to understand the interspecies connection of this virus better.
has issue date	2019-11-19(xsd:dateTime)
bibo:doi	10.14202/vetworld.2019.1797-1805
bibo:pmid	32009759
has license	cc-by
sha1sum (hex)	cc03e915afeb7acfe93595b75a38f7b58bf2d752
schema:url	https://doi.org/10.14202/vetworld.2019.1797-1805
resource representing a document's title	Isolation and characterization of avian coronavirus from healthy Eclectus parrots (Eclectus roratus) from Indonesia
has PubMed Central identifier	PMC6925039
has PubMed identifier	32009759
schema:publication	Vet World
resource representing a document's body	covid:cc03e915afeb7acfe93595b75a38f7b58bf2d752#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/cc03e915afeb7acfe93595b75a38f7b58bf2d752
is schema:about of	named entity 'CAUSES' named entity 'CHICKENS' named entity 'TURKEYS' named entity 'BIRDS' named entity 'AUSTRALIA' named entity 'BIRD' named entity 'ISOLATED' named entity 'PETS' named entity 'VIRUS' named entity 'ENDEMIC' named entity 'ECLECTUS' named entity 'TO CHARACTERIZE' named entity 'ECLECTUS PARROT' named entity 'Eclectus roratus' covid:arg/cc03e915afeb7acfe93595b75a38f7b58bf2d752 named entity 'ECONOMICALLY' named entity 'PARROTS' named entity 'BACKGROUND' named entity 'VARIOUS' named entity 'NORTHERN' named entity 'STUDY' named entity 'CORONAVIRUS INFECTION' named entity 'INDONESIA' named entity 'ECLECTUS' named entity 'AVIAN CORONAVIRUS' named entity 'HEALTHY' named entity 'ISOLATION' named entity 'CHARACTERIZATION' named entity 'DETERMINE' named entity 'SPECIES' named entity 'AIM' named entity 'REQUIRED' named entity 'LIMITED' named entity 'HOSTS' named entity 'WILD' named entity 'AVIAN CORONAVIRUS' named entity 'ABOUT' named entity 'POSSIBLY' named entity 'RANGE' named entity 'INFORMATION' named entity 'PARROTS' named entity 'INDONESIA' named entity 'LOSS' named entity 'POULTRY INDUSTRY' named entity 'WIDE' named entity 'RESEARCH' named entity 'IMPORTANT' named entity 'PRESENT' named entity 'coronavirus' named entity 'avian' named entity 'birds' named entity 'avian' named entity 'Therefore' named entity 'Avian' named entity 'avian' named entity 'study' named entity 'loss' named entity 'Indonesia' named entity 'infection' named entity 'turkeys' named entity 'Eclectus parrot' named entity 'Avian coronavirus' named entity 'Eclectus parrots' named entity 'Indonesia' named entity 'avian coronavirus' named entity 'Eclectus roratus' named entity '3' untranslated region' named entity 'genome' named entity 'virus' named entity 'avian coronavirus' named entity 'nucleotides' named entity 'viruses' named entity 'Eclectus parrots' named entity 'avian coronavirus' named entity 'viruses'

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