About: Analytical Validation of a COVID-19 qRT-PCR Detection Assay Using a 384-well Format and Three Extraction Methods

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About: Analytical Validation of a COVID-19 qRT-PCR Detection Assay Using a 384-well Format and Three Extraction Methods Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Analytical Validation of a COVID-19 qRT-PCR Detection Assay Using a 384-well Format and Three Extraction Methods
Creator	Beckman, Kenneth Langlois, Ryan Auch, Benjamin Bold, Tyler Daniel, Jerry Fay, Elizabeth Fiege, Jessica Gohl, Daryl Grady, Patrick Johnson, Darrell Karnuth, Kylene Kincaid, Robyn Nelson, Andrew Schomaker, Matthew Yohe, Sophia
Source	BioRxiv
abstract	The COVID-19 global pandemic is an unprecedented health emergency. Insufficient access to testing has hampered effective public health interventions and patient care management in a number of countries. Furthermore, the availability of regulatory-cleared reagents has challenged widespread implementation of testing. We rapidly developed a qRT-PCR SARS-CoV-2 detection assay using a 384-well format and tested its analytic performance across multiple nucleic acid extraction kits. Our data shows robust analytic accuracy on residual clinical biospecimens. Limit of detection sensitivity and specificity was confirmed with currently available commercial reagents. Our methods and results provide valuable information for other high-complexity laboratories seeking to develop effective, local, laboratory-developed procedures with high-throughput capability to detect SARS-CoV-2.
has issue date	2020-04-05(xsd:dateTime)
bibo:doi	10.1101/2020.04.02.022186
has license	biorxiv
sha1sum (hex)	cbd28b1afccac57474af082914f0e676d1b45055
schema:url	https://doi.org/10.1101/2020.04.02.022186
resource representing a document's title	Analytical Validation of a COVID-19 qRT-PCR Detection Assay Using a 384-well Format and Three Extraction Methods
schema:publication	bioRxiv
resource representing a document's body	covid:cbd28b1afccac57474af082914f0e676d1b45055#body_text
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